A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth
To quantify dynamic protein synthesis rates, we developed MITNCAT, a method combining multiplexed isobaric mass tagging with pulsed SILAC (pSILAC)and bio-orthogonal non-canonical amino acid tagging (BONCAT)to label newly synthesized proteins with azidohomoalanine (Aha), thus enabling high temporal r...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2020
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| Online Access: | https://hdl.handle.net/1721.1/126460 |