Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging
A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and absorption prohibit non-invasive multi-photon approaches for deep (...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
The Optical Society
2020
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| Online Access: | https://hdl.handle.net/1721.1/126696 |
| _version_ | 1826190299755446272 |
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| author | Ohayon, Shay DiCarlo, James |
| author2 | McGovern Institute for Brain Research at MIT |
| author_facet | McGovern Institute for Brain Research at MIT Ohayon, Shay DiCarlo, James |
| author_sort | Ohayon, Shay |
| collection | MIT |
| description | A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and absorption prohibit non-invasive multi-photon approaches for deep (>2mm) structures, while gradient refractive index (GRIN) endoscopes are relatively thick and can cause significant damage upon insertion. Here, we present a novel micro-endoscope design to image neural activity at arbitrary depths via an ultra-thin multi-mode optical fiber (MMF) probe that has 5-10X thinner diameter than commercially available microendoscopes. We demonstrate micron-scale resolution, multi-spectral and volumetric imaging. In contrast to previous approaches, we show that this method has an improved acquisition speed that is sufficient to capture rapid neuronal dynamics in-vivo in rodents expressing a genetically encoded calcium indicator (GCaMP). Our results emphasize the potential of this technology in neuroscience applications and open up possibilities for cellular resolution imaging in previously unreachable brain regions. |
| first_indexed | 2024-09-23T08:38:09Z |
| format | Article |
| id | mit-1721.1/126696 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:38:09Z |
| publishDate | 2020 |
| publisher | The Optical Society |
| record_format | dspace |
| spelling | mit-1721.1/1266962022-09-30T10:07:39Z Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging Ohayon, Shay DiCarlo, James McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and absorption prohibit non-invasive multi-photon approaches for deep (>2mm) structures, while gradient refractive index (GRIN) endoscopes are relatively thick and can cause significant damage upon insertion. Here, we present a novel micro-endoscope design to image neural activity at arbitrary depths via an ultra-thin multi-mode optical fiber (MMF) probe that has 5-10X thinner diameter than commercially available microendoscopes. We demonstrate micron-scale resolution, multi-spectral and volumetric imaging. In contrast to previous approaches, we show that this method has an improved acquisition speed that is sufficient to capture rapid neuronal dynamics in-vivo in rodents expressing a genetically encoded calcium indicator (GCaMP). Our results emphasize the potential of this technology in neuroscience applications and open up possibilities for cellular resolution imaging in previously unreachable brain regions. National Institutes of Health (U.S.) (Grant (REY026436A) 2020-08-20T11:16:55Z 2020-08-20T11:16:55Z 2018-04 2018-02 2019-09-30T17:15:23Z Article http://purl.org/eprint/type/JournalArticle 2156-7085 https://hdl.handle.net/1721.1/126696 Ohayon, Shay et al. “Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging.” Biomedical optics express, vol. 9, no. 4, 2018, article 315080 © 2018 The Author(s) en 10.1364/BOE.9.001492 Biomedical optics express Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf The Optical Society OSA Publishing |
| spellingShingle | Ohayon, Shay DiCarlo, James Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging |
| title | Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging |
| title_full | Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging |
| title_fullStr | Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging |
| title_full_unstemmed | Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging |
| title_short | Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging |
| title_sort | minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging |
| url | https://hdl.handle.net/1721.1/126696 |
| work_keys_str_mv | AT ohayonshay minimallyinvasivemultimodeopticalfibermicroendoscopefordeepbrainfluorescenceimaging AT dicarlojames minimallyinvasivemultimodeopticalfibermicroendoscopefordeepbrainfluorescenceimaging |