Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters
Coronavirus disease 2019 (COVID-19) in humans is often a clinically mild illness, but some individuals develop severe pneumonia, respiratory failure and death. Studies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in hamsters and nonhuman primates have generally reported...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
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2020
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| Online Access: | https://hdl.handle.net/1721.1/127202 |
| _version_ | 1826212130882322432 |
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| author | Tostanoski, Lisa H. Wegmann, Frank Martinot, Amanda J. Loos, Carolin McMahan, Katherine Mercado, Noe B. Yu, Jingyou Chan, Chi N. Bondoc, Stephen Starke, Carly E. Nekorchuk, Michael Busman-Sahay, Kathleen Piedra-Mora, Cesar Wrijil, Linda M. Ducat, Sarah Custers, Jerome Atyeo, Caroline Fischinger, Stephanie Burke, John S. Feldman, Jared Hauser, Blake M. Caradonna, Timothy M. Bondzie, Esther A. Dagotto, Gabriel Gebre, Makda S. Jacob-Dolan, Catherine Lin, Zijin Mahrokhian, Shant H. Nampanya, Felix Nityanandam, Ramya Pessaint, Laurent Porto, Maciel Ali, Vaneesha Benetiene, Dalia Tevi, Komlan Andersen, Hanne Lewis, Mark G. Schmidt, Aaron G. Lauffenburger, Douglas A Alter, Galit Estes, Jacob D. Schuitemaker, Hanneke Zahn, Roland Barouch, Dan H. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Tostanoski, Lisa H. Wegmann, Frank Martinot, Amanda J. Loos, Carolin McMahan, Katherine Mercado, Noe B. Yu, Jingyou Chan, Chi N. Bondoc, Stephen Starke, Carly E. Nekorchuk, Michael Busman-Sahay, Kathleen Piedra-Mora, Cesar Wrijil, Linda M. Ducat, Sarah Custers, Jerome Atyeo, Caroline Fischinger, Stephanie Burke, John S. Feldman, Jared Hauser, Blake M. Caradonna, Timothy M. Bondzie, Esther A. Dagotto, Gabriel Gebre, Makda S. Jacob-Dolan, Catherine Lin, Zijin Mahrokhian, Shant H. Nampanya, Felix Nityanandam, Ramya Pessaint, Laurent Porto, Maciel Ali, Vaneesha Benetiene, Dalia Tevi, Komlan Andersen, Hanne Lewis, Mark G. Schmidt, Aaron G. Lauffenburger, Douglas A Alter, Galit Estes, Jacob D. Schuitemaker, Hanneke Zahn, Roland Barouch, Dan H. |
| author_sort | Tostanoski, Lisa H. |
| collection | MIT |
| description | Coronavirus disease 2019 (COVID-19) in humans is often a clinically mild illness, but some individuals develop severe pneumonia, respiratory failure and death. Studies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in hamsters and nonhuman primates have generally reported mild clinical disease, and preclinical SARS-CoV-2 vaccine studies have demonstrated reduction of viral replication in the upper and lower respiratory tracts in nonhuman primates. Here we show that high-dose intranasal SARS-CoV-2 infection in hamsters results in severe clinical disease, including high levels of virus replication in tissues, extensive pneumonia, weight loss and mortality in a subset of animals. A single immunization with an adenovirus serotype 26 vector-based vaccine expressing a stabilized SARS-CoV-2 spike protein elicited binding and neutralizing antibody responses and protected against SARS-CoV-2-induced weight loss, pneumonia and mortality. These data demonstrate vaccine protection against SARS-CoV-2 clinical disease. This model should prove useful for preclinical studies of SARS-CoV-2 vaccines, therapeutics and pathogenesis. |
| first_indexed | 2024-09-23T15:16:33Z |
| format | Article |
| id | mit-1721.1/127202 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:16:33Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | mit-1721.1/1272022022-10-02T01:53:33Z Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters Tostanoski, Lisa H. Wegmann, Frank Martinot, Amanda J. Loos, Carolin McMahan, Katherine Mercado, Noe B. Yu, Jingyou Chan, Chi N. Bondoc, Stephen Starke, Carly E. Nekorchuk, Michael Busman-Sahay, Kathleen Piedra-Mora, Cesar Wrijil, Linda M. Ducat, Sarah Custers, Jerome Atyeo, Caroline Fischinger, Stephanie Burke, John S. Feldman, Jared Hauser, Blake M. Caradonna, Timothy M. Bondzie, Esther A. Dagotto, Gabriel Gebre, Makda S. Jacob-Dolan, Catherine Lin, Zijin Mahrokhian, Shant H. Nampanya, Felix Nityanandam, Ramya Pessaint, Laurent Porto, Maciel Ali, Vaneesha Benetiene, Dalia Tevi, Komlan Andersen, Hanne Lewis, Mark G. Schmidt, Aaron G. Lauffenburger, Douglas A Alter, Galit Estes, Jacob D. Schuitemaker, Hanneke Zahn, Roland Barouch, Dan H. Massachusetts Institute of Technology. Department of Biological Engineering Coronavirus disease 2019 (COVID-19) in humans is often a clinically mild illness, but some individuals develop severe pneumonia, respiratory failure and death. Studies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in hamsters and nonhuman primates have generally reported mild clinical disease, and preclinical SARS-CoV-2 vaccine studies have demonstrated reduction of viral replication in the upper and lower respiratory tracts in nonhuman primates. Here we show that high-dose intranasal SARS-CoV-2 infection in hamsters results in severe clinical disease, including high levels of virus replication in tissues, extensive pneumonia, weight loss and mortality in a subset of animals. A single immunization with an adenovirus serotype 26 vector-based vaccine expressing a stabilized SARS-CoV-2 spike protein elicited binding and neutralizing antibody responses and protected against SARS-CoV-2-induced weight loss, pneumonia and mortality. These data demonstrate vaccine protection against SARS-CoV-2 clinical disease. This model should prove useful for preclinical studies of SARS-CoV-2 vaccines, therapeutics and pathogenesis. 2020-09-08T21:07:15Z 2020-09-08T21:07:15Z 2020-09 2020-08 Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/127202 Tostanoski, Lisa H. et al. "Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters." Nature Medicine (September 2020): doi.org/10.1038/s41591-020-1070-6 © 2020 Springer Nature https://doi.org/10.1038/s41591-020-1070-6 Nature Medicine Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Nature |
| spellingShingle | Tostanoski, Lisa H. Wegmann, Frank Martinot, Amanda J. Loos, Carolin McMahan, Katherine Mercado, Noe B. Yu, Jingyou Chan, Chi N. Bondoc, Stephen Starke, Carly E. Nekorchuk, Michael Busman-Sahay, Kathleen Piedra-Mora, Cesar Wrijil, Linda M. Ducat, Sarah Custers, Jerome Atyeo, Caroline Fischinger, Stephanie Burke, John S. Feldman, Jared Hauser, Blake M. Caradonna, Timothy M. Bondzie, Esther A. Dagotto, Gabriel Gebre, Makda S. Jacob-Dolan, Catherine Lin, Zijin Mahrokhian, Shant H. Nampanya, Felix Nityanandam, Ramya Pessaint, Laurent Porto, Maciel Ali, Vaneesha Benetiene, Dalia Tevi, Komlan Andersen, Hanne Lewis, Mark G. Schmidt, Aaron G. Lauffenburger, Douglas A Alter, Galit Estes, Jacob D. Schuitemaker, Hanneke Zahn, Roland Barouch, Dan H. Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters |
| title | Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters |
| title_full | Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters |
| title_fullStr | Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters |
| title_full_unstemmed | Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters |
| title_short | Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters |
| title_sort | ad26 vaccine protects against sars cov 2 severe clinical disease in hamsters |
| url | https://hdl.handle.net/1721.1/127202 |
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