Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays
Immobilizing microscale objects (e.g., cells, spheroids, and microparticles) in arrays for direct observation and analysis is a critical step of many biological and chemical assays; however, existing techniques are often limited in their ability to precisely capture, arrange, isolate, and recollect...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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AIP Publishing
2020
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| Online Access: | https://hdl.handle.net/1721.1/127672 |
| _version_ | 1826189551312306176 |
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| author | Chen, Lynna Kim, Jae Jung Doyle, Patrick S |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Chen, Lynna Kim, Jae Jung Doyle, Patrick S |
| author_sort | Chen, Lynna |
| collection | MIT |
| description | Immobilizing microscale objects (e.g., cells, spheroids, and microparticles) in arrays for direct observation and analysis is a critical step of many biological and chemical assays; however, existing techniques are often limited in their ability to precisely capture, arrange, isolate, and recollect objects of interest. In this work, we present a microfluidic platform that selectively parks microparticles in hydrodynamic traps based on particle physical characteristics (size, stiffness, and internal structure). We present an accompanying scaling analysis for the particle parking process to enable rational design of microfluidic traps and selection of operating conditions for successful parking of desired particles with specific size and elastic modulus. Our platform also enables parking of encoded particle pairs in defined spatial arrangements and subsequent isolation of these pairs in aqueous droplets, creating distinct microenvironments with no cross-contamination. In addition, we demonstrate the ability to recollect objects of interest (i.e., one particle from each pair) after observation within the channel. This integrated device is ideal for multiplexed assays or microenvironment fabrication for controlled biological studies. ©2018 Author(s). |
| first_indexed | 2024-09-23T08:17:37Z |
| format | Article |
| id | mit-1721.1/127672 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:17:37Z |
| publishDate | 2020 |
| publisher | AIP Publishing |
| record_format | dspace |
| spelling | mit-1721.1/1276722022-09-23T12:06:26Z Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays Chen, Lynna Kim, Jae Jung Doyle, Patrick S Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Immobilizing microscale objects (e.g., cells, spheroids, and microparticles) in arrays for direct observation and analysis is a critical step of many biological and chemical assays; however, existing techniques are often limited in their ability to precisely capture, arrange, isolate, and recollect objects of interest. In this work, we present a microfluidic platform that selectively parks microparticles in hydrodynamic traps based on particle physical characteristics (size, stiffness, and internal structure). We present an accompanying scaling analysis for the particle parking process to enable rational design of microfluidic traps and selection of operating conditions for successful parking of desired particles with specific size and elastic modulus. Our platform also enables parking of encoded particle pairs in defined spatial arrangements and subsequent isolation of these pairs in aqueous droplets, creating distinct microenvironments with no cross-contamination. In addition, we demonstrate the ability to recollect objects of interest (i.e., one particle from each pair) after observation within the channel. This integrated device is ideal for multiplexed assays or microenvironment fabrication for controlled biological studies. ©2018 Author(s). NIH Grant (1R21EB024101-01) 2020-09-21T21:30:06Z 2020-09-21T21:30:06Z 2018-03 2017-10 2019-08-16T17:59:33Z Article http://purl.org/eprint/type/JournalArticle 1932-1058 https://hdl.handle.net/1721.1/127672 Chen, Lynna et al., "Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays." Biomicrofluidics 12, 2 (March 2018): 024102 doi. 10.1063/1.5011342 ©2018 Author(s) en https://dx.doi.org/10.1063/1.5011342 Biomicrofluidics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf AIP Publishing PMC |
| spellingShingle | Chen, Lynna Kim, Jae Jung Doyle, Patrick S Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays |
| title | Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays |
| title_full | Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays |
| title_fullStr | Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays |
| title_full_unstemmed | Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays |
| title_short | Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays |
| title_sort | microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays |
| url | https://hdl.handle.net/1721.1/127672 |
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