Development and validation of a rapid lateral flow E1/E2-antigen test and ELISA in patients infected with emerging Asian strain of Chikungunya virus in the Americas
Since its 2013 emergence in the Americas, Chikungunya virus (CHIKV) has posed a serious threat to public health. Early and accurate diagnosis of the disease, though currently lacking in clinics, is integral to enable timely care and epidemiological response. We developed a dual detection system: a C...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | https://hdl.handle.net/1721.1/127759 |
| _version_ | 1826213087456264192 |
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| author | Reddy, Ankita Bosch, Irene Salcedo, Nol Herrera, Bobby Brooke de Puig Guixe, Helena Narváez, Carlos F. Caicedo-Borrero, Diana María Lorenzana, Ivette Parham, Leda García, Kimberly Mercado, Marcela Turca, Angélica María Rico Villar-Centeno, Luis A. Gélvez-Ramírez, Margarita Ríos, Natalia Andrea Gómez Hiley, Megan Jeanne Garcia Blanco, Dawlyn J Diamond, Michael S. Gehrke, Lee |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Reddy, Ankita Bosch, Irene Salcedo, Nol Herrera, Bobby Brooke de Puig Guixe, Helena Narváez, Carlos F. Caicedo-Borrero, Diana María Lorenzana, Ivette Parham, Leda García, Kimberly Mercado, Marcela Turca, Angélica María Rico Villar-Centeno, Luis A. Gélvez-Ramírez, Margarita Ríos, Natalia Andrea Gómez Hiley, Megan Jeanne Garcia Blanco, Dawlyn J Diamond, Michael S. Gehrke, Lee |
| author_sort | Reddy, Ankita |
| collection | MIT |
| description | Since its 2013 emergence in the Americas, Chikungunya virus (CHIKV) has posed a serious threat to public health. Early and accurate diagnosis of the disease, though currently lacking in clinics, is integral to enable timely care and epidemiological response. We developed a dual detection system: a CHIKV antigen E1/E2-based enzyme-linked immunosorbent assay (ELISA) and a lateral flow test using high-affinity anti-CHIKV antibodies. The ELISA was validated with 100 PCR-tested acute Chikungunya fever samples from Honduras. The assay had an overall sensitivity and specificity of 51% and 96.67%, respectively, with accuracy reaching 95.45% sensitivity and 92.03% specificity at a cycle threshold (Ct) cutoff of 22. As the Ct value decreased from 35 to 22, the ELISA sensitivity increased. We then developed and validated two lateral flow tests using independent antibody pairs. The sensitivity and specificity reached 100% for both lateral flow tests using 39 samples from Colombia and Honduras at Ct cutoffs of 20 and 27, respectively. For both lateral flow tests, sensitivity decreased as the Ct increased after 27. Because CHIKV E1/E2 are exposed in the virion surfaces in serum during the acute infection phase, these sensitive and specific assays demonstrate opportunities for early detection of this emerging human pathogen. |
| first_indexed | 2024-09-23T15:43:28Z |
| format | Article |
| id | mit-1721.1/127759 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:43:28Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | mit-1721.1/1277592022-10-02T03:38:42Z Development and validation of a rapid lateral flow E1/E2-antigen test and ELISA in patients infected with emerging Asian strain of Chikungunya virus in the Americas Reddy, Ankita Bosch, Irene Salcedo, Nol Herrera, Bobby Brooke de Puig Guixe, Helena Narváez, Carlos F. Caicedo-Borrero, Diana María Lorenzana, Ivette Parham, Leda García, Kimberly Mercado, Marcela Turca, Angélica María Rico Villar-Centeno, Luis A. Gélvez-Ramírez, Margarita Ríos, Natalia Andrea Gómez Hiley, Megan Jeanne Garcia Blanco, Dawlyn J Diamond, Michael S. Gehrke, Lee Massachusetts Institute of Technology. Institute for Medical Engineering & Science Since its 2013 emergence in the Americas, Chikungunya virus (CHIKV) has posed a serious threat to public health. Early and accurate diagnosis of the disease, though currently lacking in clinics, is integral to enable timely care and epidemiological response. We developed a dual detection system: a CHIKV antigen E1/E2-based enzyme-linked immunosorbent assay (ELISA) and a lateral flow test using high-affinity anti-CHIKV antibodies. The ELISA was validated with 100 PCR-tested acute Chikungunya fever samples from Honduras. The assay had an overall sensitivity and specificity of 51% and 96.67%, respectively, with accuracy reaching 95.45% sensitivity and 92.03% specificity at a cycle threshold (Ct) cutoff of 22. As the Ct value decreased from 35 to 22, the ELISA sensitivity increased. We then developed and validated two lateral flow tests using independent antibody pairs. The sensitivity and specificity reached 100% for both lateral flow tests using 39 samples from Colombia and Honduras at Ct cutoffs of 20 and 27, respectively. For both lateral flow tests, sensitivity decreased as the Ct increased after 27. Because CHIKV E1/E2 are exposed in the virion surfaces in serum during the acute infection phase, these sensitive and specific assays demonstrate opportunities for early detection of this emerging human pathogen. U.S. Public Health Service (award AI100190) 2020-09-28T14:38:13Z 2020-09-28T14:38:13Z 2020-09 2020-07 2020-09-07T22:00:26Z Article http://purl.org/eprint/type/JournalArticle 1999-4915 https://hdl.handle.net/1721.1/127759 Reddy, Ankita et al. "Development and validation of a rapid lateral flow E1/E2-antigen test and ELISA in patients infected with emerging Asian strain of Chikungunya virus in the Americas." Viruses 12, 9 (September 2020): 971 ©2020 Author(s) 10.3390/v12090971 Viruses Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Multidisciplinary Digital Publishing Institute Multidisciplinary Digital Publishing Institute |
| spellingShingle | Reddy, Ankita Bosch, Irene Salcedo, Nol Herrera, Bobby Brooke de Puig Guixe, Helena Narváez, Carlos F. Caicedo-Borrero, Diana María Lorenzana, Ivette Parham, Leda García, Kimberly Mercado, Marcela Turca, Angélica María Rico Villar-Centeno, Luis A. Gélvez-Ramírez, Margarita Ríos, Natalia Andrea Gómez Hiley, Megan Jeanne Garcia Blanco, Dawlyn J Diamond, Michael S. Gehrke, Lee Development and validation of a rapid lateral flow E1/E2-antigen test and ELISA in patients infected with emerging Asian strain of Chikungunya virus in the Americas |
| title | Development and validation of a rapid lateral flow E1/E2-antigen test and ELISA in patients infected with emerging Asian strain of Chikungunya virus in the Americas |
| title_full | Development and validation of a rapid lateral flow E1/E2-antigen test and ELISA in patients infected with emerging Asian strain of Chikungunya virus in the Americas |
| title_fullStr | Development and validation of a rapid lateral flow E1/E2-antigen test and ELISA in patients infected with emerging Asian strain of Chikungunya virus in the Americas |
| title_full_unstemmed | Development and validation of a rapid lateral flow E1/E2-antigen test and ELISA in patients infected with emerging Asian strain of Chikungunya virus in the Americas |
| title_short | Development and validation of a rapid lateral flow E1/E2-antigen test and ELISA in patients infected with emerging Asian strain of Chikungunya virus in the Americas |
| title_sort | development and validation of a rapid lateral flow e1 e2 antigen test and elisa in patients infected with emerging asian strain of chikungunya virus in the americas |
| url | https://hdl.handle.net/1721.1/127759 |
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