Cell swelling, softening and invasion in a three-dimensional breast cancer model
Control of the structure and function of three-dimensional multicellular tissues depends critically on the spatial and temporal coordination of cellular physical properties, yet the organizational principles that govern these events and their disruption in disease remain poorly understood. Using a m...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer
2020
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| Online Access: | https://hdl.handle.net/1721.1/127818 |
| _version_ | 1811069376507412480 |
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| author | Han, Yulong Pegoraro, Adrian F. Li, Hui Li, Kaifu Yuan, Yuan Xu, Guoqiang Gu, Zichen Sun, Jiawei Hao, Yukun Gupta, Satish Kumar Li, Yiwei Tang, Wenhui Kang, Hua Teng, Lianghong Fredberg, Jeffrey J. Guo, Ming |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Han, Yulong Pegoraro, Adrian F. Li, Hui Li, Kaifu Yuan, Yuan Xu, Guoqiang Gu, Zichen Sun, Jiawei Hao, Yukun Gupta, Satish Kumar Li, Yiwei Tang, Wenhui Kang, Hua Teng, Lianghong Fredberg, Jeffrey J. Guo, Ming |
| author_sort | Han, Yulong |
| collection | MIT |
| description | Control of the structure and function of three-dimensional multicellular tissues depends critically on the spatial and temporal coordination of cellular physical properties, yet the organizational principles that govern these events and their disruption in disease remain poorly understood. Using a multicellular mammary cancer organoid model, we map here the spatial and temporal evolution of positions, motions and physical characteristics of individual cells in three dimensions. Compared with cells in the organoid core, cells at the organoid periphery and the invasive front are found to be systematically softer, larger and more dynamic. These mechanical changes are shown to arise from supracellular fluid flow through gap junctions, the suppression of which delays the transition to an invasive phenotype. These findings highlight the role of spatiotemporal coordination of cellular physical properties in tissue organization and disease progression. |
| first_indexed | 2024-09-23T08:10:02Z |
| format | Article |
| id | mit-1721.1/127818 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:10:02Z |
| publishDate | 2020 |
| publisher | Springer |
| record_format | dspace |
| spelling | mit-1721.1/1278182022-09-23T11:22:36Z Cell swelling, softening and invasion in a three-dimensional breast cancer model Han, Yulong Pegoraro, Adrian F. Li, Hui Li, Kaifu Yuan, Yuan Xu, Guoqiang Gu, Zichen Sun, Jiawei Hao, Yukun Gupta, Satish Kumar Li, Yiwei Tang, Wenhui Kang, Hua Teng, Lianghong Fredberg, Jeffrey J. Guo, Ming Massachusetts Institute of Technology. Department of Mechanical Engineering Control of the structure and function of three-dimensional multicellular tissues depends critically on the spatial and temporal coordination of cellular physical properties, yet the organizational principles that govern these events and their disruption in disease remain poorly understood. Using a multicellular mammary cancer organoid model, we map here the spatial and temporal evolution of positions, motions and physical characteristics of individual cells in three dimensions. Compared with cells in the organoid core, cells at the organoid periphery and the invasive front are found to be systematically softer, larger and more dynamic. These mechanical changes are shown to arise from supracellular fluid flow through gap junctions, the suppression of which delays the transition to an invasive phenotype. These findings highlight the role of spatiotemporal coordination of cellular physical properties in tissue organization and disease progression. National Cancer Institute (Grant 1U01CA202123) 2020-10-06T21:28:54Z 2020-10-06T21:28:54Z 2019-10 2018-09 2020-09-21T15:07:24Z Article http://purl.org/eprint/type/JournalArticle 1745-2473 1745-2481 https://hdl.handle.net/1721.1/127818 Han, Yu Long et al. "Cell swelling, softening and invasion in a three-dimensional breast cancer model." Nature Physics 16, 1 (October 2019): 101–108 © 2019 The Author(s) en http://dx.doi.org/10.1038/s41567-019-0680-8 Nature Physics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Springer Prof. Guo via Elizabeth Soergel |
| spellingShingle | Han, Yulong Pegoraro, Adrian F. Li, Hui Li, Kaifu Yuan, Yuan Xu, Guoqiang Gu, Zichen Sun, Jiawei Hao, Yukun Gupta, Satish Kumar Li, Yiwei Tang, Wenhui Kang, Hua Teng, Lianghong Fredberg, Jeffrey J. Guo, Ming Cell swelling, softening and invasion in a three-dimensional breast cancer model |
| title | Cell swelling, softening and invasion in a three-dimensional breast cancer model |
| title_full | Cell swelling, softening and invasion in a three-dimensional breast cancer model |
| title_fullStr | Cell swelling, softening and invasion in a three-dimensional breast cancer model |
| title_full_unstemmed | Cell swelling, softening and invasion in a three-dimensional breast cancer model |
| title_short | Cell swelling, softening and invasion in a three-dimensional breast cancer model |
| title_sort | cell swelling softening and invasion in a three dimensional breast cancer model |
| url | https://hdl.handle.net/1721.1/127818 |
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