Cellular expression through morphogen delivery by light activated magnetic microrobots
Microrobots have many potential uses in microbiology since they can be remotely actuated and precisely manipulated in biochemical fluids. Cellular function and response depends on biochemicals. Therefore, various delivery methods have been developed for delivering biologically relevant cargo using m...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Springer Science and Business Media
2020
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| Online Access: | https://hdl.handle.net/1721.1/128381 |
| _version_ | 1826198361366069248 |
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| author | Das, Sambeeta Hunter, Elizabeth E. DeLateur, Nicholas Andrew Steager, Edward B. Weiss, Ron Kumar, Vijay |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Das, Sambeeta Hunter, Elizabeth E. DeLateur, Nicholas Andrew Steager, Edward B. Weiss, Ron Kumar, Vijay |
| author_sort | Das, Sambeeta |
| collection | MIT |
| description | Microrobots have many potential uses in microbiology since they can be remotely actuated and precisely manipulated in biochemical fluids. Cellular function and response depends on biochemicals. Therefore, various delivery methods have been developed for delivering biologically relevant cargo using microrobots. However, localized targeting without payload leakage during transport is challenging. Here, we design a microrobotic platform capable of on-demand delivery of signaling molecules in biological systems. The on-demand delivery method is based on a light-responsive photolabile linker which releases a cell-to-cell signaling molecule when exposed to light, integrated on the surface of microrobots. Successful delivery of the signaling molecules and subsequent gene regulation is also demonstrated. This proposed method can be used for multiple applications, especially in biology, engineering, and medicine where on-demand delivery of chemical cargo at targeted locations is important. |
| first_indexed | 2024-09-23T11:03:39Z |
| format | Article |
| id | mit-1721.1/128381 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:03:39Z |
| publishDate | 2020 |
| publisher | Springer Science and Business Media |
| record_format | dspace |
| spelling | mit-1721.1/1283812022-10-01T00:52:58Z Cellular expression through morphogen delivery by light activated magnetic microrobots Das, Sambeeta Hunter, Elizabeth E. DeLateur, Nicholas Andrew Steager, Edward B. Weiss, Ron Kumar, Vijay Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Biological Engineering Microrobots have many potential uses in microbiology since they can be remotely actuated and precisely manipulated in biochemical fluids. Cellular function and response depends on biochemicals. Therefore, various delivery methods have been developed for delivering biologically relevant cargo using microrobots. However, localized targeting without payload leakage during transport is challenging. Here, we design a microrobotic platform capable of on-demand delivery of signaling molecules in biological systems. The on-demand delivery method is based on a light-responsive photolabile linker which releases a cell-to-cell signaling molecule when exposed to light, integrated on the surface of microrobots. Successful delivery of the signaling molecules and subsequent gene regulation is also demonstrated. This proposed method can be used for multiple applications, especially in biology, engineering, and medicine where on-demand delivery of chemical cargo at targeted locations is important. ONR (Grant N00014-11-1-0725) NSF (Grants CNS-1446474 and CNS-1446592) 2020-11-05T23:38:08Z 2020-11-05T23:38:08Z 2019-06 2019-05 2020-09-24T21:05:53Z Article http://purl.org/eprint/type/JournalArticle 2194-6418 2194-6426 https://hdl.handle.net/1721.1/128381 Das, Sambeeta et al. "Cellular expression through morphogen delivery by light activated magnetic microrobots." Journal of Micro-Bio Robotics 15, 2 (June 2019): 79–90 © 2019 Springer-Verlag en http://dx.doi.org/10.1007/s12213-019-00119-x Journal of Micro-Bio Robotics Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ Springer-Verlag GmbH Germany, part of Springer Nature application/pdf Springer Science and Business Media Springer Berlin Heidelberg |
| spellingShingle | Das, Sambeeta Hunter, Elizabeth E. DeLateur, Nicholas Andrew Steager, Edward B. Weiss, Ron Kumar, Vijay Cellular expression through morphogen delivery by light activated magnetic microrobots |
| title | Cellular expression through morphogen delivery by light activated magnetic microrobots |
| title_full | Cellular expression through morphogen delivery by light activated magnetic microrobots |
| title_fullStr | Cellular expression through morphogen delivery by light activated magnetic microrobots |
| title_full_unstemmed | Cellular expression through morphogen delivery by light activated magnetic microrobots |
| title_short | Cellular expression through morphogen delivery by light activated magnetic microrobots |
| title_sort | cellular expression through morphogen delivery by light activated magnetic microrobots |
| url | https://hdl.handle.net/1721.1/128381 |
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