Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics
A fully-Automated and portable leukocyte separation platform was developed based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) device, as an alternative to centrifugation. By combining key innovations in inertial microfluidic device designs and check-valve-bas...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Royal Society of Chemistry (RSC)
2020
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| Online Access: | https://hdl.handle.net/1721.1/128431 |
| _version_ | 1826216571231535104 |
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| author | Jeon, Hyungkook Jundi, Bakr Choi, Kyungyong Ryu, Hyunryul Levy, Bruce D. Lim, Geunbae Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Research Laboratory of Electronics |
| author_facet | Massachusetts Institute of Technology. Research Laboratory of Electronics Jeon, Hyungkook Jundi, Bakr Choi, Kyungyong Ryu, Hyunryul Levy, Bruce D. Lim, Geunbae Han, Jongyoon |
| author_sort | Jeon, Hyungkook |
| collection | MIT |
| description | A fully-Automated and portable leukocyte separation platform was developed based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) device, as an alternative to centrifugation. By combining key innovations in inertial microfluidic device designs and check-valve-based recirculation processes, highly purified and concentrated WBCs (up to >99.99% RBC removal, ∼80% WBC recovery, >85% WBC purity, and ∼12-fold concentrated WBCs compared to the input sample) were achieved in less than 5 minutes, with high reliability and repeatability (coefficient of variation, CV < 5%). Using this, one can harvest up to 0.4 million of intact WBCs from 50 μL of human peripheral blood (50 μL), without any cell damage or phenotypic changes in a fully-Automated operation. Alternatively, hand-powered operation is demonstrated with comparable separation efficiency and speed, which eliminates the need for electricity altogether for truly field-friendly sample preparation. The proposed platform is therefore highly deployable for various point-of-care applications, including bedside assessment of the host immune response and blood sample processing in resource-limited environments. |
| first_indexed | 2024-09-23T16:49:42Z |
| format | Article |
| id | mit-1721.1/128431 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:49:42Z |
| publishDate | 2020 |
| publisher | Royal Society of Chemistry (RSC) |
| record_format | dspace |
| spelling | mit-1721.1/1284312022-10-03T08:35:40Z Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics Jeon, Hyungkook Jundi, Bakr Choi, Kyungyong Ryu, Hyunryul Levy, Bruce D. Lim, Geunbae Han, Jongyoon Massachusetts Institute of Technology. Research Laboratory of Electronics Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Biological Engineering A fully-Automated and portable leukocyte separation platform was developed based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) device, as an alternative to centrifugation. By combining key innovations in inertial microfluidic device designs and check-valve-based recirculation processes, highly purified and concentrated WBCs (up to >99.99% RBC removal, ∼80% WBC recovery, >85% WBC purity, and ∼12-fold concentrated WBCs compared to the input sample) were achieved in less than 5 minutes, with high reliability and repeatability (coefficient of variation, CV < 5%). Using this, one can harvest up to 0.4 million of intact WBCs from 50 μL of human peripheral blood (50 μL), without any cell damage or phenotypic changes in a fully-Automated operation. Alternatively, hand-powered operation is demonstrated with comparable separation efficiency and speed, which eliminates the need for electricity altogether for truly field-friendly sample preparation. The proposed platform is therefore highly deployable for various point-of-care applications, including bedside assessment of the host immune response and blood sample processing in resource-limited environments. 2020-11-09T21:07:05Z 2020-11-09T21:07:05Z 2020-08 2020-07 2020-10-19T12:21:30Z Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 https://hdl.handle.net/1721.1/128431 Jeon, Hyungkook et al. "Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS)." Lab on a Chip 20, 19 (August 2020): 3612-3624 © The Royal Society of Chemistry en http://dx.doi.org/10.1039/d0lc00675k Lab on a Chip Creative Commons Attribution Noncommercial 3.0 unported license https://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry (RSC) Royal Society of Chemistry (RSC) |
| spellingShingle | Jeon, Hyungkook Jundi, Bakr Choi, Kyungyong Ryu, Hyunryul Levy, Bruce D. Lim, Geunbae Han, Jongyoon Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics |
| title | Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics |
| title_full | Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics |
| title_fullStr | Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics |
| title_full_unstemmed | Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics |
| title_short | Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics |
| title_sort | fully automated and field deployable blood leukocyte separation platform using multi dimensional double spiral mdds inertial microfluidics |
| url | https://hdl.handle.net/1721.1/128431 |
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