Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation
Synaptic connections between hippocampal mossy fibers (MFs) and CA3 pyramidal neurons are essential for contextual memory encoding, but the molecular mechanisms regulating MF-CA3 synapses during memory formation and the exact nature of this regulation are poorly understood. Here we report that the a...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2020
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| Online Access: | https://hdl.handle.net/1721.1/128839 |
| _version_ | 1826213664033603584 |
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| author | Weng, Feng-Ju Garcia, Rodrigo Lutzu, Stefano Alviña, Karina Zhang, Yuxiang Dushko, Margaret Ku, Taeyun Zemoura, Khaled Rich, David Garcia-Dominguez, Dario Hung, Matthew Yelhekar, Tushar D Sørensen, Andreas Toft Xu, Weifeng Chung, Kwanghun Castillo, Pablo E. Lin, Yingxi |
| author2 | McGovern Institute for Brain Research at MIT |
| author_facet | McGovern Institute for Brain Research at MIT Weng, Feng-Ju Garcia, Rodrigo Lutzu, Stefano Alviña, Karina Zhang, Yuxiang Dushko, Margaret Ku, Taeyun Zemoura, Khaled Rich, David Garcia-Dominguez, Dario Hung, Matthew Yelhekar, Tushar D Sørensen, Andreas Toft Xu, Weifeng Chung, Kwanghun Castillo, Pablo E. Lin, Yingxi |
| author_sort | Weng, Feng-Ju |
| collection | MIT |
| description | Synaptic connections between hippocampal mossy fibers (MFs) and CA3 pyramidal neurons are essential for contextual memory encoding, but the molecular mechanisms regulating MF-CA3 synapses during memory formation and the exact nature of this regulation are poorly understood. Here we report that the activity-dependent transcription factor Npas4 selectively regulates the structure and strength of MF-CA3 synapses by restricting the number of their functional synaptic contacts without affecting the other synaptic inputs onto CA3 pyramidal neurons. Using an activity-dependent reporter, we identified CA3 pyramidal cells that were activated by contextual learning and found that MF inputs on these cells were selectively strengthened. Deletion of Npas4 prevented both contextual memory formation and this learning-induced synaptic modification. We further show that Npas4 regulates MF-CA3 synapses by controlling the expression of the polo-like kinase Plk2. Thus, Npas4 is a critical regulator of experience-dependent, structural, and functional plasticity at MF-CA3 synapses during contextual memory formation. Weng et al. report that the transcription factor Npas4 selectively regulates the number of functional synaptic contacts between CA3 pyramidal neurons and mossy fibers, allowing for learning-induced modification of MF-CA3 synapses during contextual memory formation. |
| first_indexed | 2024-09-23T15:52:51Z |
| format | Article |
| id | mit-1721.1/128839 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:52:51Z |
| publishDate | 2020 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1288392022-10-02T04:49:11Z Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation Weng, Feng-Ju Garcia, Rodrigo Lutzu, Stefano Alviña, Karina Zhang, Yuxiang Dushko, Margaret Ku, Taeyun Zemoura, Khaled Rich, David Garcia-Dominguez, Dario Hung, Matthew Yelhekar, Tushar D Sørensen, Andreas Toft Xu, Weifeng Chung, Kwanghun Castillo, Pablo E. Lin, Yingxi McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Chemical Engineering Harvard University--MIT Division of Health Sciences and Technology Synaptic connections between hippocampal mossy fibers (MFs) and CA3 pyramidal neurons are essential for contextual memory encoding, but the molecular mechanisms regulating MF-CA3 synapses during memory formation and the exact nature of this regulation are poorly understood. Here we report that the activity-dependent transcription factor Npas4 selectively regulates the structure and strength of MF-CA3 synapses by restricting the number of their functional synaptic contacts without affecting the other synaptic inputs onto CA3 pyramidal neurons. Using an activity-dependent reporter, we identified CA3 pyramidal cells that were activated by contextual learning and found that MF inputs on these cells were selectively strengthened. Deletion of Npas4 prevented both contextual memory formation and this learning-induced synaptic modification. We further show that Npas4 regulates MF-CA3 synapses by controlling the expression of the polo-like kinase Plk2. Thus, Npas4 is a critical regulator of experience-dependent, structural, and functional plasticity at MF-CA3 synapses during contextual memory formation. Weng et al. report that the transcription factor Npas4 selectively regulates the number of functional synaptic contacts between CA3 pyramidal neurons and mossy fibers, allowing for learning-induced modification of MF-CA3 synapses during contextual memory formation. NIH (Grants DA017392, NS090473, MH081935, MH091220, NS088421, and DC014701) 2020-12-15T22:43:11Z 2020-12-15T22:43:11Z 2018-03 2017-11 2019-08-16T13:21:24Z Article http://purl.org/eprint/type/JournalArticle 0896-6273 https://hdl.handle.net/1721.1/128839 Weng, Feng-Ju et al. "Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation." Neuron 97, 5 (March 2018): 1137-1152.e5 © 2018 Elsevier Inc en http://dx.doi.org/10.1016/j.neuron.2018.01.026 Neuron Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Weng, Feng-Ju Garcia, Rodrigo Lutzu, Stefano Alviña, Karina Zhang, Yuxiang Dushko, Margaret Ku, Taeyun Zemoura, Khaled Rich, David Garcia-Dominguez, Dario Hung, Matthew Yelhekar, Tushar D Sørensen, Andreas Toft Xu, Weifeng Chung, Kwanghun Castillo, Pablo E. Lin, Yingxi Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation |
| title | Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation |
| title_full | Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation |
| title_fullStr | Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation |
| title_full_unstemmed | Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation |
| title_short | Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation |
| title_sort | npas4 is a critical regulator of learning induced plasticity at mossy fiber ca3 synapses during contextual memory formation |
| url | https://hdl.handle.net/1721.1/128839 |
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