Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip
Long-term management for leukemia is challenging due to the painful and invasive procedure of bone marrow (BM) biopsy. At present, non-invasive liquid (blood) biopsy is not utilized for leukemia, due to lower counts of leukemia blast cells in the blood. Here, we described a robust system for the sim...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Springer
2020
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| Online Access: | https://hdl.handle.net/1721.1/128873 |
| _version_ | 1826206404105469952 |
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| author | Khoo, Bee Luan Shang, Menglin Ng, Chin Hin Lim, Chwee-Teck Chng, Wee Joo Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Khoo, Bee Luan Shang, Menglin Ng, Chin Hin Lim, Chwee-Teck Chng, Wee Joo Han, Jongyoon |
| author_sort | Khoo, Bee Luan |
| collection | MIT |
| description | Long-term management for leukemia is challenging due to the painful and invasive procedure of bone marrow (BM) biopsy. At present, non-invasive liquid (blood) biopsy is not utilized for leukemia, due to lower counts of leukemia blast cells in the blood. Here, we described a robust system for the simultaneous detection and enrichment of rare blast cells. Enrichment of blast cells was achieved from blood with a one-step microfluidic blast cell biochip (BCB) sorting system, without specific targeting of proteins by antibodies. Non-target cells encountered a differential net force as compared to stiffer blast cells and were removed. The efficiency of the BCB promotes high detection sensitivity (1 in 10<jats:sup>6</jats:sup> cells) even from patients with minimal residual disease. The procedure was validated using actual blast cells from patients with various types of leukemia. Outcomes were compared to current evaluation standards, such as flow cytometry, using BM aspirates. Blast cell detection efficiency was higher in 55.6% of the patients using the BCB as compared to flow cytometry, despite the lower concentrations of blast cells in liquid biopsy. These studies promote early-stage detection and routine monitoring for minimal residual disease in patients. |
| first_indexed | 2024-09-23T13:29:15Z |
| format | Article |
| id | mit-1721.1/128873 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:29:15Z |
| publishDate | 2020 |
| publisher | Springer |
| record_format | dspace |
| spelling | mit-1721.1/1288732022-10-01T15:32:49Z Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip Khoo, Bee Luan Shang, Menglin Ng, Chin Hin Lim, Chwee-Teck Chng, Wee Joo Han, Jongyoon Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Biological Engineering Singapore-MIT Alliance in Research and Technology (SMART) Long-term management for leukemia is challenging due to the painful and invasive procedure of bone marrow (BM) biopsy. At present, non-invasive liquid (blood) biopsy is not utilized for leukemia, due to lower counts of leukemia blast cells in the blood. Here, we described a robust system for the simultaneous detection and enrichment of rare blast cells. Enrichment of blast cells was achieved from blood with a one-step microfluidic blast cell biochip (BCB) sorting system, without specific targeting of proteins by antibodies. Non-target cells encountered a differential net force as compared to stiffer blast cells and were removed. The efficiency of the BCB promotes high detection sensitivity (1 in 10<jats:sup>6</jats:sup> cells) even from patients with minimal residual disease. The procedure was validated using actual blast cells from patients with various types of leukemia. Outcomes were compared to current evaluation standards, such as flow cytometry, using BM aspirates. Blast cell detection efficiency was higher in 55.6% of the patients using the BCB as compared to flow cytometry, despite the lower concentrations of blast cells in liquid biopsy. These studies promote early-stage detection and routine monitoring for minimal residual disease in patients. 2020-12-21T16:19:56Z 2020-12-21T16:19:56Z 2019-12 2019-06 2020-12-17T13:16:12Z Article http://purl.org/eprint/type/JournalArticle 2397-768X https://hdl.handle.net/1721.1/128873 Khoo, Bee Luan et al. "Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip." npj Precision Oncology 3, 1 (December 2019): 30 en http://dx.doi.org/10.1038/s41698-019-0102-5 npj Precision Oncology Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Nature |
| spellingShingle | Khoo, Bee Luan Shang, Menglin Ng, Chin Hin Lim, Chwee-Teck Chng, Wee Joo Han, Jongyoon Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip |
| title | Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip |
| title_full | Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip |
| title_fullStr | Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip |
| title_full_unstemmed | Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip |
| title_short | Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip |
| title_sort | liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip |
| url | https://hdl.handle.net/1721.1/128873 |
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