Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces
Peripheral and integral membrane proteins feature in stepwise assembly of complex glycans and glycoconjugates. Catalysis on membrane-bound substrates features challenges with substrate solubility and active-site accessibility. However, advantages in enzyme and substrate orientation and control of la...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Elsevier BV
2020
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| Online Access: | https://hdl.handle.net/1721.1/128917 |
| _version_ | 1826211760313466880 |
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| author | Allen, Karen N Imperiali, Barbara |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Allen, Karen N Imperiali, Barbara |
| author_sort | Allen, Karen N |
| collection | MIT |
| description | Peripheral and integral membrane proteins feature in stepwise assembly of complex glycans and glycoconjugates. Catalysis on membrane-bound substrates features challenges with substrate solubility and active-site accessibility. However, advantages in enzyme and substrate orientation and control of lateral membrane diffusion provide order to the multistep processes. Recent glycosyltransferase (GT) studies show that substrate diversity is met by the selection of folds which do not converge upon a common mechanism. Examples of polyprenol phosphate phosphoglycosyl transferases (PGTs) highlight that divergent fold families catalyze the same reaction with different mechanisms. Lipid A biosynthesis enzymes illustrate that variations on the robust Rossmann fold allow substrate diversity. Improved understanding of GT and PGT structure and function holds promise for better function prediction and improvement of therapeutic inhibitory ligands. |
| first_indexed | 2024-09-23T15:11:00Z |
| format | Article |
| id | mit-1721.1/128917 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:11:00Z |
| publishDate | 2020 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1289172022-09-29T13:13:35Z Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces Allen, Karen N Imperiali, Barbara Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Peripheral and integral membrane proteins feature in stepwise assembly of complex glycans and glycoconjugates. Catalysis on membrane-bound substrates features challenges with substrate solubility and active-site accessibility. However, advantages in enzyme and substrate orientation and control of lateral membrane diffusion provide order to the multistep processes. Recent glycosyltransferase (GT) studies show that substrate diversity is met by the selection of folds which do not converge upon a common mechanism. Examples of polyprenol phosphate phosphoglycosyl transferases (PGTs) highlight that divergent fold families catalyze the same reaction with different mechanisms. Lipid A biosynthesis enzymes illustrate that variations on the robust Rossmann fold allow substrate diversity. Improved understanding of GT and PGT structure and function holds promise for better function prediction and improvement of therapeutic inhibitory ligands. National Institutes of Health (Grant R01-GM131627) 2020-12-23T20:08:29Z 2020-12-23T20:08:29Z 2019-04 2020-12-16T15:45:16Z Article http://purl.org/eprint/type/JournalArticle 0959-440X https://hdl.handle.net/1721.1/128917 Allen, Karen N and Barbara Imperiali. "Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces." Current Opinion in Structural Biology 59 (December 2019): 81-90 © 2019 Elsevier Ltd en http://dx.doi.org/10.1016/j.sbi.2019.03.013 Current Opinion in Structural Biology Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Allen, Karen N Imperiali, Barbara Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces |
| title | Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces |
| title_full | Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces |
| title_fullStr | Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces |
| title_full_unstemmed | Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces |
| title_short | Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces |
| title_sort | structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces |
| url | https://hdl.handle.net/1721.1/128917 |
| work_keys_str_mv | AT allenkarenn structuralandmechanisticthemesinglycoconjugatebiosynthesisatmembraneinterfaces AT imperialibarbara structuralandmechanisticthemesinglycoconjugatebiosynthesisatmembraneinterfaces |