Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins
Mohs surgery uses en face frozen section analysis (FSA) with complete margin examination for the excision of select basal cell carcinomas (BCC), obtaining excellent cosmetic outcomes and extremely low recurrence rates. However, Mohs with FSA is time-consuming because of the need to iteratively perfo...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Optical Society of America (OSA)
2021
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| Online Access: | https://hdl.handle.net/1721.1/128963 |
| _version_ | 1826206688739328000 |
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| author | Giacomelli, Michael Faulkner-Jones, Beverly E. Cahill, Lucas Christopher Yoshitake, Tadayuki Do, Daihung Fujimoto, James G |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Giacomelli, Michael Faulkner-Jones, Beverly E. Cahill, Lucas Christopher Yoshitake, Tadayuki Do, Daihung Fujimoto, James G |
| author_sort | Giacomelli, Michael |
| collection | MIT |
| description | Mohs surgery uses en face frozen section analysis (FSA) with complete margin examination for the excision of select basal cell carcinomas (BCC), obtaining excellent cosmetic outcomes and extremely low recurrence rates. However, Mohs with FSA is time-consuming because of the need to iteratively perform cryosectioning on sequential excisions. Fluorescent microscopies can image tissue specimens without requiring physical sectioning, potentially reducing the time to perform Mohs surgery. We demonstrate a protocol for nonlinear microscopy (NLM) imaging of surgical specimens that combines dual agent staining, virtual H&E rendering, and video rate imaging. We also introduce a novel protocol that enables micron-level co-registration of NLM images with FSA histology, and demonstrate that NLM can reproduce similar features similar to FSA in BCC specimens with both negative and positive surgical margins. We show that the fluorescent labels can be extracted with conventional vacuum infiltration processing, enabling subsequent immunohistochemistry on fluorescently labeled tissue. This protocol can also be applied to evaluate the performance of NLM compared with FSA in a wide range of pathologies for intraoperative consultation. |
| first_indexed | 2024-09-23T13:37:01Z |
| format | Article |
| id | mit-1721.1/128963 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:37:01Z |
| publishDate | 2021 |
| publisher | Optical Society of America (OSA) |
| record_format | dspace |
| spelling | mit-1721.1/1289632022-09-28T15:03:38Z Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins Giacomelli, Michael Faulkner-Jones, Beverly E. Cahill, Lucas Christopher Yoshitake, Tadayuki Do, Daihung Fujimoto, James G Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Mohs surgery uses en face frozen section analysis (FSA) with complete margin examination for the excision of select basal cell carcinomas (BCC), obtaining excellent cosmetic outcomes and extremely low recurrence rates. However, Mohs with FSA is time-consuming because of the need to iteratively perform cryosectioning on sequential excisions. Fluorescent microscopies can image tissue specimens without requiring physical sectioning, potentially reducing the time to perform Mohs surgery. We demonstrate a protocol for nonlinear microscopy (NLM) imaging of surgical specimens that combines dual agent staining, virtual H&E rendering, and video rate imaging. We also introduce a novel protocol that enables micron-level co-registration of NLM images with FSA histology, and demonstrate that NLM can reproduce similar features similar to FSA in BCC specimens with both negative and positive surgical margins. We show that the fluorescent labels can be extracted with conventional vacuum infiltration processing, enabling subsequent immunohistochemistry on fluorescently labeled tissue. This protocol can also be applied to evaluate the performance of NLM compared with FSA in a wide range of pathologies for intraoperative consultation. National Institutes of Health (Grants R01-CA075289, R01-CA178636) Air Force Office of Scientific Research (Grant FA9550-15-1-0473) 2021-01-05T21:44:53Z 2021-01-05T21:44:53Z 2019-07 2019-07 2020-12-14T20:05:15Z Article http://purl.org/eprint/type/JournalArticle 2156-7085 https://hdl.handle.net/1721.1/128963 Giacomelli, Michael G. et al. "Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins." Biomedical Optics Express 10, 8 (July 2019): 4249-4260 © 2019 Optical Society of America en http://dx.doi.org/10.1364/boe.10.004249 Biomedical Optics Express Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Optical Society of America (OSA) OSA Publishing |
| spellingShingle | Giacomelli, Michael Faulkner-Jones, Beverly E. Cahill, Lucas Christopher Yoshitake, Tadayuki Do, Daihung Fujimoto, James G Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins |
| title | Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins |
| title_full | Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins |
| title_fullStr | Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins |
| title_full_unstemmed | Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins |
| title_short | Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins |
| title_sort | comparison of nonlinear microscopy and frozen section histology for imaging of mohs surgical margins |
| url | https://hdl.handle.net/1721.1/128963 |
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