Production of d-Glyceric acid from d-Galacturonate in Escherichia coli
A microbial production platform has been developed in Escherichia coli to synthesize d-glyceric acid from d-galacturonate. The expression of uronate dehydrogenase (udh) from Pseudomonas syringae and galactarolactone isomerase (gli) from Agrobacterium fabrum, along with the inactivation of garK, enco...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press (OUP)
2021
|
| Online Access: | https://hdl.handle.net/1721.1/128964 |
| _version_ | 1826211801519357952 |
|---|---|
| author | Fox, Kevin J. Prather, Kristala L |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Fox, Kevin J. Prather, Kristala L |
| author_sort | Fox, Kevin J. |
| collection | MIT |
| description | A microbial production platform has been developed in Escherichia coli to synthesize d-glyceric acid from d-galacturonate. The expression of uronate dehydrogenase (udh) from Pseudomonas syringae and galactarolactone isomerase (gli) from Agrobacterium fabrum, along with the inactivation of garK, encoding for glycerate kinase, enables d-glyceric acid accumulation by utilizing the endogenous expression of galactarate dehydratase (garD), 5-keto-4-deoxy-D-glucarate aldolase (garL), and 2-hydroxy-3-oxopropionate reductase (garR). Optimization of carbon flux through the elimination of competing metabolic pathways led to the development of a ΔgarKΔhyiΔglxKΔuxaC mutant strain that produced 4.8 g/l of d-glyceric acid from d-galacturonate, with an 83% molar yield. Cultivation in a minimal medium produced similar yields and demonstrated that galactose or glycerol serve as possible carbon co-feeds for industrial production. This novel platform represents an alternative for the production of d-glyceric acid, an industrially relevant chemical, that addresses current challenges in using acetic acid bacteria for its synthesis: increasing yield, enantio-purity and biological stability. |
| first_indexed | 2024-09-23T15:11:37Z |
| format | Article |
| id | mit-1721.1/128964 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:11:37Z |
| publishDate | 2021 |
| publisher | Oxford University Press (OUP) |
| record_format | dspace |
| spelling | mit-1721.1/1289642022-09-29T13:17:29Z Production of d-Glyceric acid from d-Galacturonate in Escherichia coli Fox, Kevin J. Prather, Kristala L Massachusetts Institute of Technology. Department of Chemical Engineering A microbial production platform has been developed in Escherichia coli to synthesize d-glyceric acid from d-galacturonate. The expression of uronate dehydrogenase (udh) from Pseudomonas syringae and galactarolactone isomerase (gli) from Agrobacterium fabrum, along with the inactivation of garK, encoding for glycerate kinase, enables d-glyceric acid accumulation by utilizing the endogenous expression of galactarate dehydratase (garD), 5-keto-4-deoxy-D-glucarate aldolase (garL), and 2-hydroxy-3-oxopropionate reductase (garR). Optimization of carbon flux through the elimination of competing metabolic pathways led to the development of a ΔgarKΔhyiΔglxKΔuxaC mutant strain that produced 4.8 g/l of d-glyceric acid from d-galacturonate, with an 83% molar yield. Cultivation in a minimal medium produced similar yields and demonstrated that galactose or glycerol serve as possible carbon co-feeds for industrial production. This novel platform represents an alternative for the production of d-glyceric acid, an industrially relevant chemical, that addresses current challenges in using acetic acid bacteria for its synthesis: increasing yield, enantio-purity and biological stability. U.S. Army Research Office (Contract W911NF-09-0001) 2021-01-05T21:55:28Z 2021-01-05T21:55:28Z 2020-10 2020-05 2020-12-11T04:22:23Z Article http://purl.org/eprint/type/JournalArticle 1367-5435 1476-5535 https://hdl.handle.net/1721.1/128964 Fox, Kevin J. and Kristala L. J. Prather et al. "Production of d-Glyceric acid from d-Galacturonate in Escherichia coli." Journal of Industrial Microbiology and Biotechnology 47, 12 (October 2020): 1075–1081 © 2020 Society for Industrial Microbiology and Biotechnology en https://doi.org/10.1007/s10295-020-02323-2 Journal of Industrial Microbiology and Biotechnology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ Society for Industrial Microbiology and Biotechnology application/pdf Oxford University Press (OUP) Springer International Publishing |
| spellingShingle | Fox, Kevin J. Prather, Kristala L Production of d-Glyceric acid from d-Galacturonate in Escherichia coli |
| title | Production of d-Glyceric acid from d-Galacturonate in Escherichia coli |
| title_full | Production of d-Glyceric acid from d-Galacturonate in Escherichia coli |
| title_fullStr | Production of d-Glyceric acid from d-Galacturonate in Escherichia coli |
| title_full_unstemmed | Production of d-Glyceric acid from d-Galacturonate in Escherichia coli |
| title_short | Production of d-Glyceric acid from d-Galacturonate in Escherichia coli |
| title_sort | production of d glyceric acid from d galacturonate in escherichia coli |
| url | https://hdl.handle.net/1721.1/128964 |
| work_keys_str_mv | AT foxkevinj productionofdglycericacidfromdgalacturonateinescherichiacoli AT pratherkristalal productionofdglycericacidfromdgalacturonateinescherichiacoli |