Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2020
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
| Published: |
Massachusetts Institute of Technology
2021
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/1721.1/129041 |
| _version_ | 1811087735483531264 |
|---|---|
| author | Jacobowitz, Joseph(Joseph R.) |
| author2 | Jing-Ke Weng. |
| author_facet | Jing-Ke Weng. Jacobowitz, Joseph(Joseph R.) |
| author_sort | Jacobowitz, Joseph(Joseph R.) |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2020 |
| first_indexed | 2024-09-23T13:51:11Z |
| format | Thesis |
| id | mit-1721.1/129041 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T13:51:11Z |
| publishDate | 2021 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1290412021-01-06T03:15:39Z Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers Jacobowitz, Joseph(Joseph R.) Jing-Ke Weng. Massachusetts Institute of Technology. Department of Biology. Massachusetts Institute of Technology. Department of Biology Biology. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2020 Cataloged from student-submitted PDF of thesis. Includes bibliographical references. When aquatic plants migrated to land 500 million years ago, they were met with harsh conditions associated with terrestrial life, such as dry air, high radiance light, and high effective gravity. Early plants of this time period underwent rapid evolution to develop novel plant traits to mitigate these challenges - these traits remain highly conserved in modern land plants. Among the most important of these traits is the biosynthesis of a unique polymer known as sporopollenin, which protects the vulnerable plant spore and pollen grain. Sporopollenin is considered to be the toughest known biopolymer and although chemists and botanists have studied this remarkable material for over a century, relatively little is known about sporopollenin compared to other major plant biopolymers. In this thesis, I employ reverse genetic approaches to identify novel Arabidopsis genes that are responsible for sporopollenin biosynthesis. With these methods, I identify a previously unstudied gene, hereby known as IPE2 , which acts redundantly with IPE1 in the synthesis of sporopollenin. Additionally, I identify two unstudied peroxidases, PRX9 and PRX40 , which are also redundant and critical for pollen development, although these are not involved in sporopollenin and instead crosslink cell wall extensin proteins. These works enhance our understanding of the pollen wall and of pollen development. Moreover, this work reveals the untapped potential of reverse genetics to predict redundant relationships between paralogs in well-studied model organisms. by Joseph Jacobowitz. Ph. D. Ph.D. Massachusetts Institute of Technology, Department of Biology 2021-01-05T23:14:53Z 2021-01-05T23:14:53Z 2020 2020 Thesis https://hdl.handle.net/1721.1/129041 1227031226 eng MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided. http://dspace.mit.edu/handle/1721.1/7582 117 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biology. Jacobowitz, Joseph(Joseph R.) Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers |
| title | Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers |
| title_full | Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers |
| title_fullStr | Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers |
| title_full_unstemmed | Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers |
| title_short | Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers |
| title_sort | reverse genetic approaches reveal gene redundancy in arabidopsis anthers |
| topic | Biology. |
| url | https://hdl.handle.net/1721.1/129041 |
| work_keys_str_mv | AT jacobowitzjosephjosephr reversegeneticapproachesrevealgeneredundancyinarabidopsisanthers |