Genetic Engineering by DNA Recombineering

Genetic Engineering by DNA Recombineering

Recombineering inserts PCR products into DNA using homologous recombination. A pair of short homology arms (50 base pairs) on the ends of a PCR cassette target the cassette to its intended location. These homology arms can be easily introduced as 5' primer overhangs during the PCR reaction. The...

Полное описание

Библиографические подробности
Главные авторы:	Papa, Louis John, Shoulders, Matthew D.
Другие авторы:	Massachusetts Institute of Technology. Department of Chemistry
Формат:	Статья
Язык:	English
Опубликовано:	Wiley 2021
Online-ссылка:	https://hdl.handle.net/1721.1/129451

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