Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds
Polymer scaffolds that direct elongation and orientation of cultured cells can enable tissue engineered muscle to act as a mechanically functional unit. We combined micromolding and microablation technologies to create muscle tissue engineering scaffolds from the biodegradable elastomer poly(glycero...
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| Format: | Article |
| Language: | English |
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Wiley-Blackwell
2021
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| Online Access: | https://hdl.handle.net/1721.1/129997 |
| _version_ | 1826208308547026944 |
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| author | Guillemette, Maxime D. Park, Hyoungshin Hsiao, James C. Jain, Saloni R. Larson, Benjamin L. Langer, Robert S Freed, Lisa E |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Guillemette, Maxime D. Park, Hyoungshin Hsiao, James C. Jain, Saloni R. Larson, Benjamin L. Langer, Robert S Freed, Lisa E |
| author_sort | Guillemette, Maxime D. |
| collection | MIT |
| description | Polymer scaffolds that direct elongation and orientation of cultured cells can enable tissue engineered muscle to act as a mechanically functional unit. We combined micromolding and microablation technologies to create muscle tissue engineering scaffolds from the biodegradable elastomer poly(glycerol sebacate). These scaffolds exhibited well defined surface patterns and pores and robust elastomeric tensile mechanical properties. Cultured C2C12 muscle cells penetrated the pores to form spatially controlled engineered tissues. Scanning electron and confocal microscopy revealed muscle cell orientation in a preferential direction, parallel to micromolded gratings and long axes of microablated anisotropic pores, with significant individual and interactive effects of gratings and pore design.Micropatterning and microablation technologies were combined in the context of the biodegradable elastomer PGS to create a muscle tissue engineering scaffold. Scaffolds enabled cultured muscle cells to preferentially align in parallel to linear gratings and pore edges, with significant individual and interactive effects of surface topography and anisotropic pore design. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| first_indexed | 2024-09-23T14:03:45Z |
| format | Article |
| id | mit-1721.1/129997 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:03:45Z |
| publishDate | 2021 |
| publisher | Wiley-Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1299972022-09-28T18:06:08Z Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds Guillemette, Maxime D. Park, Hyoungshin Hsiao, James C. Jain, Saloni R. Larson, Benjamin L. Langer, Robert S Freed, Lisa E Harvard University--MIT Division of Health Sciences and Technology Polymer scaffolds that direct elongation and orientation of cultured cells can enable tissue engineered muscle to act as a mechanically functional unit. We combined micromolding and microablation technologies to create muscle tissue engineering scaffolds from the biodegradable elastomer poly(glycerol sebacate). These scaffolds exhibited well defined surface patterns and pores and robust elastomeric tensile mechanical properties. Cultured C2C12 muscle cells penetrated the pores to form spatially controlled engineered tissues. Scanning electron and confocal microscopy revealed muscle cell orientation in a preferential direction, parallel to micromolded gratings and long axes of microablated anisotropic pores, with significant individual and interactive effects of gratings and pore design.Micropatterning and microablation technologies were combined in the context of the biodegradable elastomer PGS to create a muscle tissue engineering scaffold. Scaffolds enabled cultured muscle cells to preferentially align in parallel to linear gratings and pore edges, with significant individual and interactive effects of surface topography and anisotropic pore design. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. American Recovery and Reinvestment Act - ARRA (1-R01-HL086521-01A2) NIH (DE013023) NSF (BES-0609182) 2021-02-24T20:44:32Z 2021-02-24T20:44:32Z 2010-08 2010-06 2019-08-22T18:56:11Z Article http://purl.org/eprint/type/JournalArticle 1616-5195 https://hdl.handle.net/1721.1/129997 Guillemette, Maxime D. et al., "Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds." Macromolecular Bioscience 10, 11 (November 2010): 1330-37 ©2010 Authors en https://dx.doi.org/10.1002/mabi.201000165 Macromolecular Bioscience Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley-Blackwell PMC |
| spellingShingle | Guillemette, Maxime D. Park, Hyoungshin Hsiao, James C. Jain, Saloni R. Larson, Benjamin L. Langer, Robert S Freed, Lisa E Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds |
| title | Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds |
| title_full | Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds |
| title_fullStr | Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds |
| title_full_unstemmed | Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds |
| title_short | Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds |
| title_sort | combined technologies for microfabricating elastomeric cardiac tissue engineering scaffolds |
| url | https://hdl.handle.net/1721.1/129997 |
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