Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans
Animal behaviors are commonly organized into long-lasting states that coordinately impact the generation of diverse motor outputs such as feeding, locomotion, and grooming. However, the neural mechanisms that coordinate these distinct motor programs remain poorly understood. Here, we examine how the...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications, Ltd
2021
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| Online Access: | https://hdl.handle.net/1721.1/130265 |
| _version_ | 1811086001318133760 |
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| author | Cermak, Nathan Yu, Stephanie K. Clark, Rebekah I. Huang, Yung-Chi Baskoylu, Saba N Flavell, Steven W Flavell, Steven Willem |
| author2 | Picower Institute for Learning and Memory |
| author_facet | Picower Institute for Learning and Memory Cermak, Nathan Yu, Stephanie K. Clark, Rebekah I. Huang, Yung-Chi Baskoylu, Saba N Flavell, Steven W Flavell, Steven Willem |
| author_sort | Cermak, Nathan |
| collection | MIT |
| description | Animal behaviors are commonly organized into long-lasting states that coordinately impact the generation of diverse motor outputs such as feeding, locomotion, and grooming. However, the neural mechanisms that coordinate these distinct motor programs remain poorly understood. Here, we examine how the distinct motor programs of the nematode C. elegans are coupled together across behavioral states. We describe a new imaging platform that permits automated, simultaneous quantification of each of the main C. elegans motor programs over hours or days. Analysis of these whole-organism behavioral profiles shows that the motor programs coordinately change as animals switch behavioral states. Utilizing genetics, optogenetics, and calcium imaging, we identify a new role for dopamine in coupling locomotion and egg-laying together across states. These results provide new insights into how the diverse motor programs throughout an organism are coordinated and suggest that neuromodulators like dopamine can couple motor circuits together in a state-dependent manner. |
| first_indexed | 2024-09-23T13:19:20Z |
| format | Article |
| id | mit-1721.1/130265 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:19:20Z |
| publishDate | 2021 |
| publisher | eLife Sciences Publications, Ltd |
| record_format | dspace |
| spelling | mit-1721.1/1302652022-09-28T13:22:19Z Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans Cermak, Nathan Yu, Stephanie K. Clark, Rebekah I. Huang, Yung-Chi Baskoylu, Saba N Flavell, Steven W Flavell, Steven Willem Picower Institute for Learning and Memory Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Animal behaviors are commonly organized into long-lasting states that coordinately impact the generation of diverse motor outputs such as feeding, locomotion, and grooming. However, the neural mechanisms that coordinate these distinct motor programs remain poorly understood. Here, we examine how the distinct motor programs of the nematode C. elegans are coupled together across behavioral states. We describe a new imaging platform that permits automated, simultaneous quantification of each of the main C. elegans motor programs over hours or days. Analysis of these whole-organism behavioral profiles shows that the motor programs coordinately change as animals switch behavioral states. Utilizing genetics, optogenetics, and calcium imaging, we identify a new role for dopamine in coupling locomotion and egg-laying together across states. These results provide new insights into how the diverse motor programs throughout an organism are coordinated and suggest that neuromodulators like dopamine can couple motor circuits together in a state-dependent manner. National Science Foundation (Grants IOS 1845663, DUE 1845663) National Institutes of Health (Grant NS104892) 2021-03-29T20:22:07Z 2021-03-29T20:22:07Z 2020-06 2020-03 2021-03-16T13:34:37Z Article http://purl.org/eprint/type/JournalArticle 2050-084X https://hdl.handle.net/1721.1/130265 Cermak, Nathan et al. "Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans." eLife 9 (June 2020): e57093 © 2020 Cermak et al. en http://dx.doi.org/10.7554/elife.57093 eLife Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf eLife Sciences Publications, Ltd eLife |
| spellingShingle | Cermak, Nathan Yu, Stephanie K. Clark, Rebekah I. Huang, Yung-Chi Baskoylu, Saba N Flavell, Steven W Flavell, Steven Willem Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans |
| title | Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans |
| title_full | Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans |
| title_fullStr | Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans |
| title_full_unstemmed | Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans |
| title_short | Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans |
| title_sort | whole organism behavioral profiling reveals a role for dopamine in state dependent motor program coupling in c elegans |
| url | https://hdl.handle.net/1721.1/130265 |
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