Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury
Neural stem cell (NSC) grafts have demonstrated significant effects in animal models of spinal cord injury (SCI), yet their clinical translation remains challenging. Significant evidence suggests that the supporting matrix of NSC grafts has a crucial role in regulating NSC effects. Here we demonstra...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/130538 |
| _version_ | 1811081556467384320 |
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| author | Kourgiantaki, Alexandra Tzeranis, Dimitrios Karali, Kanelina Georgelou, Konstantina Bampoula, Efstathia Psilodimitrakopoulos, Sotirios Yannas, Ioannis V Stratakis, Emmanuel Sidiropoulou, Kyriaki Charalampopoulos, Ioannis Gravanis, Achille |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Kourgiantaki, Alexandra Tzeranis, Dimitrios Karali, Kanelina Georgelou, Konstantina Bampoula, Efstathia Psilodimitrakopoulos, Sotirios Yannas, Ioannis V Stratakis, Emmanuel Sidiropoulou, Kyriaki Charalampopoulos, Ioannis Gravanis, Achille |
| author_sort | Kourgiantaki, Alexandra |
| collection | MIT |
| description | Neural stem cell (NSC) grafts have demonstrated significant effects in animal models of spinal cord injury (SCI), yet their clinical translation remains challenging. Significant evidence suggests that the supporting matrix of NSC grafts has a crucial role in regulating NSC effects. Here we demonstrate that grafts based on porous collagen-based scaffolds (PCSs), similar to biomaterials utilized clinically in induced regeneration, can deliver and protect embryonic NSCs at SCI sites, leading to significant improvement in locomotion recovery in an experimental mouse SCI model, so that 12 weeks post-injury locomotion performance of implanted animals does not statistically differ from that of uninjured control animals. NSC-seeded PCS grafts can modulate key processes required to induce regeneration in SCI lesions including enhancing NSC neuronal differentiation and functional integration in vivo, enabling robust axonal elongation, and reducing astrogliosis. Our findings suggest that the efficacy and translational potential of emerging NSC-based SCI therapies could be enhanced by delivering NSC via scaffolds derived from well-characterized clinically proven PCS. |
| first_indexed | 2024-09-23T11:48:29Z |
| format | Article |
| id | mit-1721.1/130538 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:48:29Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1305382022-10-01T06:10:00Z Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury Kourgiantaki, Alexandra Tzeranis, Dimitrios Karali, Kanelina Georgelou, Konstantina Bampoula, Efstathia Psilodimitrakopoulos, Sotirios Yannas, Ioannis V Stratakis, Emmanuel Sidiropoulou, Kyriaki Charalampopoulos, Ioannis Gravanis, Achille Massachusetts Institute of Technology. Department of Mechanical Engineering Neural stem cell (NSC) grafts have demonstrated significant effects in animal models of spinal cord injury (SCI), yet their clinical translation remains challenging. Significant evidence suggests that the supporting matrix of NSC grafts has a crucial role in regulating NSC effects. Here we demonstrate that grafts based on porous collagen-based scaffolds (PCSs), similar to biomaterials utilized clinically in induced regeneration, can deliver and protect embryonic NSCs at SCI sites, leading to significant improvement in locomotion recovery in an experimental mouse SCI model, so that 12 weeks post-injury locomotion performance of implanted animals does not statistically differ from that of uninjured control animals. NSC-seeded PCS grafts can modulate key processes required to induce regeneration in SCI lesions including enhancing NSC neuronal differentiation and functional integration in vivo, enabling robust axonal elongation, and reducing astrogliosis. Our findings suggest that the efficacy and translational potential of emerging NSC-based SCI therapies could be enhanced by delivering NSC via scaffolds derived from well-characterized clinically proven PCS. 2021-04-27T20:31:20Z 2021-04-27T20:31:20Z 2020-06 2018-08 2020-08-13T17:38:38Z Article http://purl.org/eprint/type/JournalArticle 2057-3995 https://hdl.handle.net/1721.1/130538 Kourgiantaki, Alexandra et al. "Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury." npj Regenerative Medicine 5, 1 (June 2020): 12 © 2020 The Author(s) en http://dx.doi.org/10.1038/s41536-020-0097-0 npj Regenerative Medicine Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Kourgiantaki, Alexandra Tzeranis, Dimitrios Karali, Kanelina Georgelou, Konstantina Bampoula, Efstathia Psilodimitrakopoulos, Sotirios Yannas, Ioannis V Stratakis, Emmanuel Sidiropoulou, Kyriaki Charalampopoulos, Ioannis Gravanis, Achille Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury |
| title | Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury |
| title_full | Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury |
| title_fullStr | Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury |
| title_full_unstemmed | Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury |
| title_short | Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury |
| title_sort | neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury |
| url | https://hdl.handle.net/1721.1/130538 |
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