Quantitative SARS-CoV-2 Alpha Variant B.1.1.7 Tracking in Wastewater by Allele-Specific RT-qPCR

The critical need for surveillance of SARS-CoV-2 variants of concern has prompted the development of methods that can track variants in wastewater. Here, we develop and present an open-source method based on allele-specific RT-qPCR (AS RT-qPCR) that detects and quantifies the B.1.1.7 variant, target...

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Bibliographic Details
Main Authors:	Lee, Wei Lin, Imakaev, Maxim, Armas, Federica, McElroy, Kyle A., Gu, Xiaoqiong, Duvallet, Claire, Chandra, Franciscus, Chen, Hongjie, Leifels, Mats, Mendola, Samuel, Floyd-O’Sullivan, Róisín, Powell, Morgan M., Wilson, Shane T., Berge, Karl L. J., Lim, Claire Y. J., Wu, Fuqing, Xiao, Amy, Moniz, Katya H, Ghaeli, Newsha, Matus, Mariana, Thompson, Janelle, Alm, Eric J.
Other Authors:	Massachusetts Institute of Technology. Center for Microbiome Informatics and Therapeutics
Format:	Article
Published:	American Chemical Society (ACS) 2021
Online Access:	https://hdl.handle.net/1721.1/131141

Quantitative SARS-CoV-2 alpha variant B.1.1.7 tracking in wastewater by allele-specific RT-qPCR by Lee, Wei Lin, Imakaev, Maxim, Armas, Federica, McElroy, Kyle A., Gu, Xiaoqiong, Duvallet, Claire, Chandra, Franciscus, Chen, Hongjie, Leifels, Mats, Mendola, Samuel, Floyd-O'Sullivan, Róisín, Powell, Morgan M., Wilson, Shane T., Berge, Karl L. J., Lim, Claire Y. J., Wu, Fuqing, Xiao, Amy, Moniz, Katya, Ghaeli, Newsha, Matus, Mariana, Thompson, Janelle R., Alm, Eric J.

Published 2022

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Quantitative SARS-CoV-2 Alpha Variant B.1.1.7 Tracking in Wastewater by Allele-Specific RT-qPCR

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