Wearable biosensors enabled by cell-free synthetic biology
Integrating synthetic biology into wearables could expand opportunities for noninvasive monitoring of physiological status, disease states and exposure to pathogens or toxins. However, the operation of synthetic circuits generally requires the presence of living, engineered bacteria, which has limit...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/131278 |
| _version_ | 1811083462340247552 |
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| author | Nguyen, Peter Q. Soenksen Martinez, Luis Ruben Donghia, Nina M. Angenent-Mari, Nicolaas M. de Puig Guixe, Helena Huang, Ally Lee, Rose Slomovic, Shimyn Galbersanini, Tommaso Lansberry, Geoffrey Sallum, Hani M. Zhao, Evan M. Niemi, James B. Collins, James J. |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Nguyen, Peter Q. Soenksen Martinez, Luis Ruben Donghia, Nina M. Angenent-Mari, Nicolaas M. de Puig Guixe, Helena Huang, Ally Lee, Rose Slomovic, Shimyn Galbersanini, Tommaso Lansberry, Geoffrey Sallum, Hani M. Zhao, Evan M. Niemi, James B. Collins, James J. |
| author_sort | Nguyen, Peter Q. |
| collection | MIT |
| description | Integrating synthetic biology into wearables could expand opportunities for noninvasive monitoring of physiological status, disease states and exposure to pathogens or toxins. However, the operation of synthetic circuits generally requires the presence of living, engineered bacteria, which has limited their application in wearables. Here we report lightweight, flexible substrates and textiles functionalized with freeze-dried, cell-free synthetic circuits, including CRISPR-based tools, that detect metabolites, chemicals and pathogen nucleic acid signatures. The wearable devices are activated upon rehydration from aqueous exposure events and report the presence of specific molecular targets by colorimetric changes or via an optical fiber network that detects fluorescent and luminescent outputs. The detection limits for nucleic acids rival current laboratory methods such as quantitative PCR. We demonstrate the development of a face mask with a lyophilized CRISPR sensor for wearable, noninvasive detection of SARS-CoV-2 at room temperature within 90 min, requiring no user intervention other than the press of a button. |
| first_indexed | 2024-09-23T12:33:27Z |
| format | Article |
| id | mit-1721.1/131278 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:33:27Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1312782022-09-28T08:34:24Z Wearable biosensors enabled by cell-free synthetic biology Wearable materials with embedded synthetic biology sensors for biomolecule detection Nguyen, Peter Q. Soenksen Martinez, Luis Ruben Donghia, Nina M. Angenent-Mari, Nicolaas M. de Puig Guixe, Helena Huang, Ally Lee, Rose Slomovic, Shimyn Galbersanini, Tommaso Lansberry, Geoffrey Sallum, Hani M. Zhao, Evan M. Niemi, James B. Collins, James J. Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Institute for Medical Engineering & Science Integrating synthetic biology into wearables could expand opportunities for noninvasive monitoring of physiological status, disease states and exposure to pathogens or toxins. However, the operation of synthetic circuits generally requires the presence of living, engineered bacteria, which has limited their application in wearables. Here we report lightweight, flexible substrates and textiles functionalized with freeze-dried, cell-free synthetic circuits, including CRISPR-based tools, that detect metabolites, chemicals and pathogen nucleic acid signatures. The wearable devices are activated upon rehydration from aqueous exposure events and report the presence of specific molecular targets by colorimetric changes or via an optical fiber network that detects fluorescent and luminescent outputs. The detection limits for nucleic acids rival current laboratory methods such as quantitative PCR. We demonstrate the development of a face mask with a lyophilized CRISPR sensor for wearable, noninvasive detection of SARS-CoV-2 at room temperature within 90 min, requiring no user intervention other than the press of a button. 2021-09-16T15:52:43Z 2021-09-16T15:52:43Z 2021-06 2019-11 2021-09-15T17:13:26Z Article http://purl.org/eprint/type/JournalArticle 1087-0156 1546-1696 https://hdl.handle.net/1721.1/131278 Nguyen, Peter Q. et al. "Wearable materials with embedded synthetic biology sensors for biomolecule detection." Nature Biotechnology (June 2021). en http://dx.doi.org/10.1038/s41587-021-00950-3 Nature Biotechnology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Springer Science and Business Media LLC Prof. Collins |
| spellingShingle | Nguyen, Peter Q. Soenksen Martinez, Luis Ruben Donghia, Nina M. Angenent-Mari, Nicolaas M. de Puig Guixe, Helena Huang, Ally Lee, Rose Slomovic, Shimyn Galbersanini, Tommaso Lansberry, Geoffrey Sallum, Hani M. Zhao, Evan M. Niemi, James B. Collins, James J. Wearable biosensors enabled by cell-free synthetic biology |
| title | Wearable biosensors enabled by cell-free synthetic biology |
| title_full | Wearable biosensors enabled by cell-free synthetic biology |
| title_fullStr | Wearable biosensors enabled by cell-free synthetic biology |
| title_full_unstemmed | Wearable biosensors enabled by cell-free synthetic biology |
| title_short | Wearable biosensors enabled by cell-free synthetic biology |
| title_sort | wearable biosensors enabled by cell free synthetic biology |
| url | https://hdl.handle.net/1721.1/131278 |
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