Faster Sickling Kinetics and Sickle Cell Shape Evolution during Repeated Deoxygenation and Oxygenation Cycles
Abstract Kinetics of cell sickling and morphological change have been recognized as important parameters that are correlated closely with altered blood rheology and vasoocclusion in microcirculation. A microfluidic transient hypoxia assay was developed to create repeated hypoxia-normo...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Springer US
2021
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| Online Access: | https://hdl.handle.net/1721.1/131894 |
| _version_ | 1811068884834320384 |
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| author | Du, E. Dao, M. |
| author_facet | Du, E. Dao, M. |
| author_sort | Du, E. |
| collection | MIT |
| description | Abstract
Kinetics of cell sickling and morphological change have been recognized as important parameters that are correlated closely with altered blood rheology and vasoocclusion in microcirculation. A microfluidic transient hypoxia assay was developed to create repeated hypoxia-normoxia cycles for real time observation of repetitive sickling and unsickling of freely suspended red blood cells (RBCs) from sickle cell disease patients. Cell sickling behavior and kinetics were found to be influenced by its previous sickling-unsickling processes accumulatively, where those sickled RBCs that had a history of sickling in a previous hypoxia cycle would sickle again in subsequent hypoxia/sickling cycles and the collective sickling kinetics became progressively faster (with reduced delay time and higher sickled fraction versus deoxygenation time). Individual sickled RBCs would sickle into drastically different shapes randomly in subsequent hypoxia/sickling cycles, however, the collective shape distribution retained similar characteristics. These observations indicate a gradual worsening trend in sickling kinetics over repeated hypoxia cycles, as well as a relatively stable collective shape characteristics within a limited number of hypoxia-normoxia cycles. |
| first_indexed | 2024-09-23T08:02:29Z |
| format | Article |
| id | mit-1721.1/131894 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:02:29Z |
| publishDate | 2021 |
| publisher | Springer US |
| record_format | dspace |
| spelling | mit-1721.1/1318942021-09-21T04:08:43Z Faster Sickling Kinetics and Sickle Cell Shape Evolution during Repeated Deoxygenation and Oxygenation Cycles Du, E. Dao, M. Abstract Kinetics of cell sickling and morphological change have been recognized as important parameters that are correlated closely with altered blood rheology and vasoocclusion in microcirculation. A microfluidic transient hypoxia assay was developed to create repeated hypoxia-normoxia cycles for real time observation of repetitive sickling and unsickling of freely suspended red blood cells (RBCs) from sickle cell disease patients. Cell sickling behavior and kinetics were found to be influenced by its previous sickling-unsickling processes accumulatively, where those sickled RBCs that had a history of sickling in a previous hypoxia cycle would sickle again in subsequent hypoxia/sickling cycles and the collective sickling kinetics became progressively faster (with reduced delay time and higher sickled fraction versus deoxygenation time). Individual sickled RBCs would sickle into drastically different shapes randomly in subsequent hypoxia/sickling cycles, however, the collective shape distribution retained similar characteristics. These observations indicate a gradual worsening trend in sickling kinetics over repeated hypoxia cycles, as well as a relatively stable collective shape characteristics within a limited number of hypoxia-normoxia cycles. 2021-09-20T17:30:50Z 2021-09-20T17:30:50Z 2018-11-28 2020-09-24T21:40:23Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/131894 en https://doi.org/10.1007/s11340-018-00444-5 Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. Society for Experimental Mechanics application/pdf Springer US Springer US |
| spellingShingle | Du, E. Dao, M. Faster Sickling Kinetics and Sickle Cell Shape Evolution during Repeated Deoxygenation and Oxygenation Cycles |
| title | Faster Sickling Kinetics and Sickle Cell Shape Evolution during Repeated Deoxygenation and Oxygenation Cycles |
| title_full | Faster Sickling Kinetics and Sickle Cell Shape Evolution during Repeated Deoxygenation and Oxygenation Cycles |
| title_fullStr | Faster Sickling Kinetics and Sickle Cell Shape Evolution during Repeated Deoxygenation and Oxygenation Cycles |
| title_full_unstemmed | Faster Sickling Kinetics and Sickle Cell Shape Evolution during Repeated Deoxygenation and Oxygenation Cycles |
| title_short | Faster Sickling Kinetics and Sickle Cell Shape Evolution during Repeated Deoxygenation and Oxygenation Cycles |
| title_sort | faster sickling kinetics and sickle cell shape evolution during repeated deoxygenation and oxygenation cycles |
| url | https://hdl.handle.net/1721.1/131894 |
| work_keys_str_mv | AT due fastersicklingkineticsandsicklecellshapeevolutionduringrepeateddeoxygenationandoxygenationcycles AT daom fastersicklingkineticsandsicklecellshapeevolutionduringrepeateddeoxygenationandoxygenationcycles |