Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control
© 2015 SPIE. High resolution imaging in three dimension is important for biological research. RESOLFT (Reversible Saturable Optical Fluorescence Transitions) microscopy is one technique can achieve lateral super-resolution imaging. Twophoton microscopy naturally generate high resolution in the longi...
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| Format: | Article |
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SPIE-Intl Soc Optical Eng
2021
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| Online Access: | https://hdl.handle.net/1721.1/132159 |
| _version_ | 1826210537034219520 |
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| author | Xue, Yi Rowlands, Christopher J. So, Peter T. C. |
| author_facet | Xue, Yi Rowlands, Christopher J. So, Peter T. C. |
| author_sort | Xue, Yi |
| collection | MIT |
| description | © 2015 SPIE. High resolution imaging in three dimension is important for biological research. RESOLFT (Reversible Saturable Optical Fluorescence Transitions) microscopy is one technique can achieve lateral super-resolution imaging. Twophoton microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. In this paper, we combine these two methods to realize three-dimensional high-resolution imaging. Spatial light modulator (SLM) is used as a flexible phase mask of the microscopy. Multiple super-resolution focuses as an array or in arbitrary positions could be generated by phase retrieval. This microscopy by SLM control could applied to parallel two-photon RESOLFT imaging or multiple spots tracking in high-resolution. |
| first_indexed | 2024-09-23T14:51:31Z |
| format | Article |
| id | mit-1721.1/132159 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:51:31Z |
| publishDate | 2021 |
| publisher | SPIE-Intl Soc Optical Eng |
| record_format | dspace |
| spelling | mit-1721.1/1321592021-09-21T03:17:12Z Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control Xue, Yi Rowlands, Christopher J. So, Peter T. C. © 2015 SPIE. High resolution imaging in three dimension is important for biological research. RESOLFT (Reversible Saturable Optical Fluorescence Transitions) microscopy is one technique can achieve lateral super-resolution imaging. Twophoton microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. In this paper, we combine these two methods to realize three-dimensional high-resolution imaging. Spatial light modulator (SLM) is used as a flexible phase mask of the microscopy. Multiple super-resolution focuses as an array or in arbitrary positions could be generated by phase retrieval. This microscopy by SLM control could applied to parallel two-photon RESOLFT imaging or multiple spots tracking in high-resolution. 2021-09-20T18:21:12Z 2021-09-20T18:21:12Z 2019-01-03T19:03:55Z Article http://purl.org/eprint/type/ConferencePaper https://hdl.handle.net/1721.1/132159 Xue, Yi, Christopher J. Rowlands, and Peter T. C. So. “Parallel and Flexible Imaging Using Two-Photon RESOLFT Microscopy with Spatial Light Modulator Control.” Edited by Ammasi Periasamy, Peter T. C. So, and Karsten König. Multiphoton Microscopy in the Biomedical Sciences XV (March 5, 2015). doi:10.1117/12.2078509. http://dx.doi.org/10.1117/12.2078509 Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE-Intl Soc Optical Eng SPIE |
| spellingShingle | Xue, Yi Rowlands, Christopher J. So, Peter T. C. Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control |
| title | Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control |
| title_full | Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control |
| title_fullStr | Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control |
| title_full_unstemmed | Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control |
| title_short | Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control |
| title_sort | parallel and flexible imaging using two photon resolft microscopy with spatial light modulator control |
| url | https://hdl.handle.net/1721.1/132159 |
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