Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections
Copyright © 2020 American Chemical Society. Group A Streptococcus (GAS) displays cell-surface proteins that resemble human collagen. We find that a fluorophore-labeled collagen mimetic peptide (CMP) labels GAS cells but not Escherichia coli or Bacillus subtilis cells, which lack such proteins. The C...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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American Chemical Society (ACS)
2021
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| Online Access: | https://hdl.handle.net/1721.1/132552 |
| _version_ | 1826200454206324736 |
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| author | Ellison, Aubrey J Dempwolff, Felix Kearns, Daniel B Raines, Ronald T |
| author_facet | Ellison, Aubrey J Dempwolff, Felix Kearns, Daniel B Raines, Ronald T |
| author_sort | Ellison, Aubrey J |
| collection | MIT |
| description | Copyright © 2020 American Chemical Society. Group A Streptococcus (GAS) displays cell-surface proteins that resemble human collagen. We find that a fluorophore-labeled collagen mimetic peptide (CMP) labels GAS cells but not Escherichia coli or Bacillus subtilis cells, which lack such proteins. The CMP likely engages in a heterotrimeric helix with endogenous collagen, as the nonnatural d enantiomer of the CMP does not label GAS cells. To identify a molecular target, we used reverse genetics to "knock-in"the GAS genes that encode two proteins with collagen-like domains, Scl1 and Scl2, into B. subtilis. The fluorescent CMP labels the cells of these B. subtilis strains. Moreover, these strains bind tightly to a surface of mammalian collagen. These data are consistent with streptococcal collagen forming triple helices with damaged collagen in a wound bed and thus have implications for microbial virulence. |
| first_indexed | 2024-09-23T11:36:43Z |
| format | Article |
| id | mit-1721.1/132552 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:36:43Z |
| publishDate | 2021 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1325522021-09-21T03:41:18Z Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections Ellison, Aubrey J Dempwolff, Felix Kearns, Daniel B Raines, Ronald T Copyright © 2020 American Chemical Society. Group A Streptococcus (GAS) displays cell-surface proteins that resemble human collagen. We find that a fluorophore-labeled collagen mimetic peptide (CMP) labels GAS cells but not Escherichia coli or Bacillus subtilis cells, which lack such proteins. The CMP likely engages in a heterotrimeric helix with endogenous collagen, as the nonnatural d enantiomer of the CMP does not label GAS cells. To identify a molecular target, we used reverse genetics to "knock-in"the GAS genes that encode two proteins with collagen-like domains, Scl1 and Scl2, into B. subtilis. The fluorescent CMP labels the cells of these B. subtilis strains. Moreover, these strains bind tightly to a surface of mammalian collagen. These data are consistent with streptococcal collagen forming triple helices with damaged collagen in a wound bed and thus have implications for microbial virulence. 2021-09-20T18:23:00Z 2021-09-20T18:23:00Z 2020-11-16T13:57:34Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/132552 en 10.1021/ACSINFECDIS.0C00073 ACS Infectious Diseases Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Ellison, Aubrey J Dempwolff, Felix Kearns, Daniel B Raines, Ronald T Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections |
| title | Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections |
| title_full | Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections |
| title_fullStr | Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections |
| title_full_unstemmed | Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections |
| title_short | Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections |
| title_sort | role for cell surface collagen of streptococcus pyogenes in infections |
| url | https://hdl.handle.net/1721.1/132552 |
| work_keys_str_mv | AT ellisonaubreyj roleforcellsurfacecollagenofstreptococcuspyogenesininfections AT dempwolfffelix roleforcellsurfacecollagenofstreptococcuspyogenesininfections AT kearnsdanielb roleforcellsurfacecollagenofstreptococcuspyogenesininfections AT rainesronaldt roleforcellsurfacecollagenofstreptococcuspyogenesininfections |