Microparticle parking and isolation for highly sensitive microRNA detection
Isolating small objects, such as particles, cells, and molecules, in individual aqueous droplets is useful for chemical and biological assays. We have developed a simple microfluidic platform to immobilize (park) microparticles at defined locations, and isolate particles in monodisperse droplets sur...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royal Society of Chemistry (RSC)
2021
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| Online Access: | https://hdl.handle.net/1721.1/132675 |
| _version_ | 1811083973948866560 |
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| author | Kim, Jae Jung Chen, Lynna Doyle, Patrick S |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Kim, Jae Jung Chen, Lynna Doyle, Patrick S |
| author_sort | Kim, Jae Jung |
| collection | MIT |
| description | Isolating small objects, such as particles, cells, and molecules, in individual aqueous droplets is useful for chemical and biological assays. We have developed a simple microfluidic platform to immobilize (park) microparticles at defined locations, and isolate particles in monodisperse droplets surrounded by immiscible oil. While conventional methods can only achieve stochastic encapsulation of objects within larger droplets, our in situ method ensures that a single particle is entrapped in a similar-sized droplet, with ∼95% yield for parking and isolation. This enables time-lapse studies of reactions in confined volumes and can be used to perform enzymatic amplification of a desired signal to improve the sensitivity of diagnostic assays. To demonstrate the utility of our technique, we perform highly sensitive, multiplexed microRNA detection by isolating encoded, functional hydrogel microparticles in small aqueous droplets. Non-fouling hydrogel microparticles are attractive for microRNA detection due to favorable capture kinetics. By encapsulating these particles in droplets and employing a generalizable enzyme amplification scheme, we demonstrate an order of magnitude improvement in detection sensitivity compared to a non-amplified assay. ©2017 The Royal Society of Chemistry. |
| first_indexed | 2024-09-23T12:42:34Z |
| format | Article |
| id | mit-1721.1/132675 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:42:34Z |
| publishDate | 2021 |
| publisher | Royal Society of Chemistry (RSC) |
| record_format | dspace |
| spelling | mit-1721.1/1326752022-09-28T09:35:08Z Microparticle parking and isolation for highly sensitive microRNA detection Kim, Jae Jung Chen, Lynna Doyle, Patrick S Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Biological Engineering Isolating small objects, such as particles, cells, and molecules, in individual aqueous droplets is useful for chemical and biological assays. We have developed a simple microfluidic platform to immobilize (park) microparticles at defined locations, and isolate particles in monodisperse droplets surrounded by immiscible oil. While conventional methods can only achieve stochastic encapsulation of objects within larger droplets, our in situ method ensures that a single particle is entrapped in a similar-sized droplet, with ∼95% yield for parking and isolation. This enables time-lapse studies of reactions in confined volumes and can be used to perform enzymatic amplification of a desired signal to improve the sensitivity of diagnostic assays. To demonstrate the utility of our technique, we perform highly sensitive, multiplexed microRNA detection by isolating encoded, functional hydrogel microparticles in small aqueous droplets. Non-fouling hydrogel microparticles are attractive for microRNA detection due to favorable capture kinetics. By encapsulating these particles in droplets and employing a generalizable enzyme amplification scheme, we demonstrate an order of magnitude improvement in detection sensitivity compared to a non-amplified assay. ©2017 The Royal Society of Chemistry. NIH-NCI Grant 5R21CA177393-02 2021-10-01T14:20:14Z 2021-10-01T14:20:14Z 2017-08 2017-06 2019-08-16T17:37:50Z Article http://purl.org/eprint/type/JournalArticle 1473-0189 https://hdl.handle.net/1721.1/132675 Kim, Jae Jung et al., "Microparticle parking and isolation for highly sensitive microRNA detection." Lab on a Chip 17, 18 (September 2017): 3120-28 doi. 10.1039/C7LC00653E ©2017 Authors en https://dx.doi.org/10.1039/C7LC00653E Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry (RSC) PMC |
| spellingShingle | Kim, Jae Jung Chen, Lynna Doyle, Patrick S Microparticle parking and isolation for highly sensitive microRNA detection |
| title | Microparticle parking and isolation for highly sensitive microRNA detection |
| title_full | Microparticle parking and isolation for highly sensitive microRNA detection |
| title_fullStr | Microparticle parking and isolation for highly sensitive microRNA detection |
| title_full_unstemmed | Microparticle parking and isolation for highly sensitive microRNA detection |
| title_short | Microparticle parking and isolation for highly sensitive microRNA detection |
| title_sort | microparticle parking and isolation for highly sensitive microrna detection |
| url | https://hdl.handle.net/1721.1/132675 |
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