Monosynaptic tracing: a step-by-step protocol
Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vector...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
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Elsevier BV
2021
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| Online Access: | https://hdl.handle.net/1721.1/132925 |
| _version_ | 1826198151018577920 |
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| author | Lavin, Thomas K. Jin, Lei Wickersham, Ian R. |
| author2 | McGovern Institute for Brain Research at MIT |
| author_facet | McGovern Institute for Brain Research at MIT Lavin, Thomas K. Jin, Lei Wickersham, Ian R. |
| author_sort | Lavin, Thomas K. |
| collection | MIT |
| description | Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors. |
| first_indexed | 2024-09-23T11:00:18Z |
| format | Article |
| id | mit-1721.1/132925 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:00:18Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1329252022-10-01T00:29:35Z Monosynaptic tracing: a step-by-step protocol Lavin, Thomas K. Jin, Lei Wickersham, Ian R. McGovern Institute for Brain Research at MIT Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors. National Institute of Mental Health (Awards U01MH106018, U01MH114829, and U19MH114830) 2021-10-12T16:01:51Z 2021-10-12T16:01:51Z 2019-12 2019-07 Article http://purl.org/eprint/type/JournalArticle 0891-0618 https://hdl.handle.net/1721.1/132925 Lavin, Thomas K. et al. "Monosynaptic tracing: a step-by-step protocol." Journal of Chemical Neuroanatomy 102 (December 2019): 101661. © 2019 Elsevier B.V. http://dx.doi.org/10.1016/j.jchemneu.2019.101661 Journal of Chemical Neuroanatomy Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV Ian Wickersham |
| spellingShingle | Lavin, Thomas K. Jin, Lei Wickersham, Ian R. Monosynaptic tracing: a step-by-step protocol |
| title | Monosynaptic tracing: a step-by-step protocol |
| title_full | Monosynaptic tracing: a step-by-step protocol |
| title_fullStr | Monosynaptic tracing: a step-by-step protocol |
| title_full_unstemmed | Monosynaptic tracing: a step-by-step protocol |
| title_short | Monosynaptic tracing: a step-by-step protocol |
| title_sort | monosynaptic tracing a step by step protocol |
| url | https://hdl.handle.net/1721.1/132925 |
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