Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging
<jats:title>Abstract</jats:title><jats:p>Human induced pluripotent stem cell-derived (iPSC) neural cultures offer clinically relevant models of human diseases, including Amyotrophic Lateral Sclerosis, Alzheimer’s, and Autism Spectrum Disorder. In situ characterization of the spatia...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/133113 |
| _version_ | 1811083758794702848 |
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| author | Tomov, Martin L O’Neil, Alison Abbasi, Hamdah S Cimini, Beth A Carpenter, Anne E Rubin, Lee L Bathe, Mark |
| author_facet | Tomov, Martin L O’Neil, Alison Abbasi, Hamdah S Cimini, Beth A Carpenter, Anne E Rubin, Lee L Bathe, Mark |
| author_sort | Tomov, Martin L |
| collection | MIT |
| description | <jats:title>Abstract</jats:title><jats:p>Human induced pluripotent stem cell-derived (iPSC) neural cultures offer clinically relevant models of human diseases, including Amyotrophic Lateral Sclerosis, Alzheimer’s, and Autism Spectrum Disorder. In situ characterization of the spatial-temporal evolution of cell state in 3D culture and subsequent 2D dissociated culture models based on protein expression levels and localizations is essential to understanding neural cell differentiation, disease state phenotypes, and sample-to-sample variability. Here, we apply <jats:underline>PR</jats:underline>obe-based <jats:underline>I</jats:underline>maging for <jats:underline>S</jats:underline>equential <jats:underline>M</jats:underline>ultiplexing (PRISM) to facilitate multiplexed imaging with facile, rapid exchange of imaging probes to analyze iPSC-derived cortical and motor neuron cultures that are relevant to psychiatric and neurodegenerative disease models, using over ten protein targets. Our approach permits analysis of cell differentiation, cell composition, and functional marker expression in complex stem-cell derived neural cultures. Furthermore, our approach is amenable to automation, offering in principle the ability to scale-up to dozens of protein targets and samples.</jats:p> |
| first_indexed | 2024-09-23T12:38:36Z |
| format | Article |
| id | mit-1721.1/133113 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:38:36Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1331132021-10-27T20:25:27Z Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging Tomov, Martin L O’Neil, Alison Abbasi, Hamdah S Cimini, Beth A Carpenter, Anne E Rubin, Lee L Bathe, Mark <jats:title>Abstract</jats:title><jats:p>Human induced pluripotent stem cell-derived (iPSC) neural cultures offer clinically relevant models of human diseases, including Amyotrophic Lateral Sclerosis, Alzheimer’s, and Autism Spectrum Disorder. In situ characterization of the spatial-temporal evolution of cell state in 3D culture and subsequent 2D dissociated culture models based on protein expression levels and localizations is essential to understanding neural cell differentiation, disease state phenotypes, and sample-to-sample variability. Here, we apply <jats:underline>PR</jats:underline>obe-based <jats:underline>I</jats:underline>maging for <jats:underline>S</jats:underline>equential <jats:underline>M</jats:underline>ultiplexing (PRISM) to facilitate multiplexed imaging with facile, rapid exchange of imaging probes to analyze iPSC-derived cortical and motor neuron cultures that are relevant to psychiatric and neurodegenerative disease models, using over ten protein targets. Our approach permits analysis of cell differentiation, cell composition, and functional marker expression in complex stem-cell derived neural cultures. Furthermore, our approach is amenable to automation, offering in principle the ability to scale-up to dozens of protein targets and samples.</jats:p> 2021-10-25T19:01:14Z 2021-10-25T19:01:14Z 2021 2021-08-24T18:49:24Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/133113 Tomov, Martin L, O’Neil, Alison, Abbasi, Hamdah S, Cimini, Beth A, Carpenter, Anne E et al. 2021. "Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging." Communications Biology, 4 (1). en 10.1038/s42003-021-02276-x Communications Biology Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Tomov, Martin L O’Neil, Alison Abbasi, Hamdah S Cimini, Beth A Carpenter, Anne E Rubin, Lee L Bathe, Mark Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging |
| title | Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging |
| title_full | Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging |
| title_fullStr | Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging |
| title_full_unstemmed | Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging |
| title_short | Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging |
| title_sort | resolving cell state in ipsc derived human neural samples with multiplexed fluorescence imaging |
| url | https://hdl.handle.net/1721.1/133113 |
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