Molecular diversity of glutamatergic and GABAergic synapses from multiplexed fluorescence imaging
© 2021 Danielson et al. Neuronal synapses contain hundreds of different protein species important for regulating signal transmission. Characterizing differential expression profiles of proteins within synapses in distinct regions of the brain has revealed a high degree of synaptic diversity defined...
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| Format: | Article |
| Language: | English |
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Society for Neuroscience
2021
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| Online Access: | https://hdl.handle.net/1721.1/133122 |
| _version_ | 1811077444794318848 |
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| author | Danielson, Eric Perez de Arce, Karen Cimini, Beth Wamhoff, Eike-Christian Singh, Shantanu Cottrell, Jeffrey R Carpenter, Anne E Bathe, Mark |
| author_facet | Danielson, Eric Perez de Arce, Karen Cimini, Beth Wamhoff, Eike-Christian Singh, Shantanu Cottrell, Jeffrey R Carpenter, Anne E Bathe, Mark |
| author_sort | Danielson, Eric |
| collection | MIT |
| description | © 2021 Danielson et al. Neuronal synapses contain hundreds of different protein species important for regulating signal transmission. Characterizing differential expression profiles of proteins within synapses in distinct regions of the brain has revealed a high degree of synaptic diversity defined by unique molecular organization. Multiplexed imaging of in vitro rat primary hippocampal culture models at single synapse resolution offers new opportunities for exploring synaptic reor-ganization in response to chemical and genetic perturbations. Here, we combine 12-color multiplexed fluorescence imaging with quantitative image analysis and machine learning to identify novel synaptic subtypes within excitatory and inhibitory synapses based on the expression profiles of major synaptic components. We characterize differences in the correlated expression of proteins within these subtypes and we examine how the distribution of these synapses is modified following induction of synaptic plasticity. Under chronic suppression of neuronal activity, phenotypic characterization revealed coordinated increases in both excitatory and inhibitory protein levels without changes in the distribution of synaptic subtypes, suggesting concerted events targeting glutamatergic and GABAergic synapses. Our results offer molecular insight into the mechanisms of synaptic plasticity. |
| first_indexed | 2024-09-23T10:43:08Z |
| format | Article |
| id | mit-1721.1/133122 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:43:08Z |
| publishDate | 2021 |
| publisher | Society for Neuroscience |
| record_format | dspace |
| spelling | mit-1721.1/1331222021-10-27T19:54:46Z Molecular diversity of glutamatergic and GABAergic synapses from multiplexed fluorescence imaging Danielson, Eric Perez de Arce, Karen Cimini, Beth Wamhoff, Eike-Christian Singh, Shantanu Cottrell, Jeffrey R Carpenter, Anne E Bathe, Mark © 2021 Danielson et al. Neuronal synapses contain hundreds of different protein species important for regulating signal transmission. Characterizing differential expression profiles of proteins within synapses in distinct regions of the brain has revealed a high degree of synaptic diversity defined by unique molecular organization. Multiplexed imaging of in vitro rat primary hippocampal culture models at single synapse resolution offers new opportunities for exploring synaptic reor-ganization in response to chemical and genetic perturbations. Here, we combine 12-color multiplexed fluorescence imaging with quantitative image analysis and machine learning to identify novel synaptic subtypes within excitatory and inhibitory synapses based on the expression profiles of major synaptic components. We characterize differences in the correlated expression of proteins within these subtypes and we examine how the distribution of these synapses is modified following induction of synaptic plasticity. Under chronic suppression of neuronal activity, phenotypic characterization revealed coordinated increases in both excitatory and inhibitory protein levels without changes in the distribution of synaptic subtypes, suggesting concerted events targeting glutamatergic and GABAergic synapses. Our results offer molecular insight into the mechanisms of synaptic plasticity. 2021-10-25T19:23:52Z 2021-10-25T19:23:52Z 2021 2021-08-24T18:39:12Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/133122 Danielson, Eric, Perez de Arce, Karen, Cimini, Beth, Wamhoff, Eike-Christian, Singh, Shantanu et al. 2021. "Molecular diversity of glutamatergic and GABAergic synapses from multiplexed fluorescence imaging." eNeuro, 8 (1). en 10.1523/ENEURO.0286-20.2020 eNeuro Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Society for Neuroscience Society for Neuroscience |
| spellingShingle | Danielson, Eric Perez de Arce, Karen Cimini, Beth Wamhoff, Eike-Christian Singh, Shantanu Cottrell, Jeffrey R Carpenter, Anne E Bathe, Mark Molecular diversity of glutamatergic and GABAergic synapses from multiplexed fluorescence imaging |
| title | Molecular diversity of glutamatergic and GABAergic synapses from multiplexed fluorescence imaging |
| title_full | Molecular diversity of glutamatergic and GABAergic synapses from multiplexed fluorescence imaging |
| title_fullStr | Molecular diversity of glutamatergic and GABAergic synapses from multiplexed fluorescence imaging |
| title_full_unstemmed | Molecular diversity of glutamatergic and GABAergic synapses from multiplexed fluorescence imaging |
| title_short | Molecular diversity of glutamatergic and GABAergic synapses from multiplexed fluorescence imaging |
| title_sort | molecular diversity of glutamatergic and gabaergic synapses from multiplexed fluorescence imaging |
| url | https://hdl.handle.net/1721.1/133122 |
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