Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-deoxy-7-amido-7-deazaguanosine-Modified DNA
A novel siphovirus, vB_PagS_MED16 (MED16) was isolated in Lithuania using <i>Pantoea agglomerans</i> strain BSL for the phage propagation. The double-stranded DNA genome of MED16 (46,103 bp) contains 73 predicted open reading frames (ORFs) encoding proteins, but no tRNA. Our comparative...
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| Other Authors: | |
| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2021
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| Online Access: | https://hdl.handle.net/1721.1/133207 |
| _version_ | 1826192287815696384 |
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| author | Šimoliūnienė, Monika Žukauskienė, Emilija Truncaitė, Lidija Cui, Liang Hutinet, Geoffrey Kazlauskas, Darius Kaupinis, Algirdas Skapas, Martynas Crécy-Lagard, Valérie de Dedon, Peter C. Valius, Mindaugas Meškys, Rolandas Šimoliūnas, Eugenijus |
| author2 | Singapore-MIT Alliance in Research and Technology (SMART) |
| author_facet | Singapore-MIT Alliance in Research and Technology (SMART) Šimoliūnienė, Monika Žukauskienė, Emilija Truncaitė, Lidija Cui, Liang Hutinet, Geoffrey Kazlauskas, Darius Kaupinis, Algirdas Skapas, Martynas Crécy-Lagard, Valérie de Dedon, Peter C. Valius, Mindaugas Meškys, Rolandas Šimoliūnas, Eugenijus |
| author_sort | Šimoliūnienė, Monika |
| collection | MIT |
| description | A novel siphovirus, vB_PagS_MED16 (MED16) was isolated in Lithuania using <i>Pantoea agglomerans</i> strain BSL for the phage propagation. The double-stranded DNA genome of MED16 (46,103 bp) contains 73 predicted open reading frames (ORFs) encoding proteins, but no tRNA. Our comparative sequence analysis revealed that 26 of these ORFs code for unique proteins that have no reliable identity when compared to database entries. Based on phylogenetic analysis, MED16 represents a new genus with siphovirus morphology. In total, 35 MED16 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phage–host interactions, and DNA metabolism. In addition, a gene encoding a preQ<sub>0</sub> DNA deoxyribosyltransferase (DpdA) is present in the genome of MED16 and the LC–MS/MS analysis indicates 2′-deoxy-7-amido-7-deazaguanosine (dADG)-modified phage DNA, which, to our knowledge, has never been experimentally validated in genomes of <i>Pantoea</i> phages. Thus, the data presented in this study provide new information on <i>Pantoea</i>-infecting viruses and offer novel insights into the diversity of DNA modifications in bacteriophages. |
| first_indexed | 2024-09-23T09:08:55Z |
| format | Article |
| id | mit-1721.1/133207 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T09:08:55Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | mit-1721.1/1332072023-08-07T17:56:44Z Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-deoxy-7-amido-7-deazaguanosine-Modified DNA Šimoliūnienė, Monika Žukauskienė, Emilija Truncaitė, Lidija Cui, Liang Hutinet, Geoffrey Kazlauskas, Darius Kaupinis, Algirdas Skapas, Martynas Crécy-Lagard, Valérie de Dedon, Peter C. Valius, Mindaugas Meškys, Rolandas Šimoliūnas, Eugenijus Singapore-MIT Alliance in Research and Technology (SMART) Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Center for Environmental Health Sciences A novel siphovirus, vB_PagS_MED16 (MED16) was isolated in Lithuania using <i>Pantoea agglomerans</i> strain BSL for the phage propagation. The double-stranded DNA genome of MED16 (46,103 bp) contains 73 predicted open reading frames (ORFs) encoding proteins, but no tRNA. Our comparative sequence analysis revealed that 26 of these ORFs code for unique proteins that have no reliable identity when compared to database entries. Based on phylogenetic analysis, MED16 represents a new genus with siphovirus morphology. In total, 35 MED16 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phage–host interactions, and DNA metabolism. In addition, a gene encoding a preQ<sub>0</sub> DNA deoxyribosyltransferase (DpdA) is present in the genome of MED16 and the LC–MS/MS analysis indicates 2′-deoxy-7-amido-7-deazaguanosine (dADG)-modified phage DNA, which, to our knowledge, has never been experimentally validated in genomes of <i>Pantoea</i> phages. Thus, the data presented in this study provide new information on <i>Pantoea</i>-infecting viruses and offer novel insights into the diversity of DNA modifications in bacteriophages. 2021-10-27T18:38:31Z 2021-10-27T18:38:31Z 2021-07-08 2021-07-08T14:22:27Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/133207 International Journal of Molecular Sciences 22 (14): 7333 (2021) PUBLISHER_CC http://dx.doi.org/10.3390/ijms22147333 Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Multidisciplinary Digital Publishing Institute Multidisciplinary Digital Publishing Institute |
| spellingShingle | Šimoliūnienė, Monika Žukauskienė, Emilija Truncaitė, Lidija Cui, Liang Hutinet, Geoffrey Kazlauskas, Darius Kaupinis, Algirdas Skapas, Martynas Crécy-Lagard, Valérie de Dedon, Peter C. Valius, Mindaugas Meškys, Rolandas Šimoliūnas, Eugenijus Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-deoxy-7-amido-7-deazaguanosine-Modified DNA |
| title | Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-deoxy-7-amido-7-deazaguanosine-Modified DNA |
| title_full | Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-deoxy-7-amido-7-deazaguanosine-Modified DNA |
| title_fullStr | Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-deoxy-7-amido-7-deazaguanosine-Modified DNA |
| title_full_unstemmed | Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-deoxy-7-amido-7-deazaguanosine-Modified DNA |
| title_short | Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-deoxy-7-amido-7-deazaguanosine-Modified DNA |
| title_sort | pantoea bacteriophage vb pags med16 a siphovirus containing a 2 deoxy 7 amido 7 deazaguanosine modified dna |
| url | https://hdl.handle.net/1721.1/133207 |
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