Identification of a long non-coding RNA regulator of liver carcinoma cell survival
© 2021, The Author(s). Genomic studies have significantly improved our understanding of hepatocellular carcinoma (HCC) biology and have led to the discovery of multiple protein-coding genes driving hepatocarcinogenesis. In addition, these studies have identified thousands of new non-coding transcrip...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/133329 |
| _version_ | 1811070931487948800 |
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| author | Rybakova, Yulia Gonzalez, John T Bogorad, Roman Chauhan, Vikash P Dong, Yize L Whittaker, Charles A Zatsepin, Timofei Koteliansky, Victor Anderson, Daniel G |
| author_facet | Rybakova, Yulia Gonzalez, John T Bogorad, Roman Chauhan, Vikash P Dong, Yize L Whittaker, Charles A Zatsepin, Timofei Koteliansky, Victor Anderson, Daniel G |
| author_sort | Rybakova, Yulia |
| collection | MIT |
| description | © 2021, The Author(s). Genomic studies have significantly improved our understanding of hepatocellular carcinoma (HCC) biology and have led to the discovery of multiple protein-coding genes driving hepatocarcinogenesis. In addition, these studies have identified thousands of new non-coding transcripts deregulated in HCC. We hypothesize that some of these transcripts may be involved in disease progression. Long non-coding RNAs are a large class of non-coding transcripts which participate in the regulation of virtually all cellular functions. However, a majority of lncRNAs remain dramatically understudied. Here, we applied a pooled shRNA-based screen to identify lncRNAs essential for HCC cell survival. We validated our screening results using RNAi, CRISPRi, and antisense oligonucleotides. We found a lncRNA, termed ASTILCS, that is critical for HCC cell growth and is overexpressed in tumors from HCC patients. We demonstrated that HCC cell death upon ASTILCS knockdown is associated with apoptosis induction and downregulation of a neighboring gene, protein tyrosine kinase 2 (PTK2), a crucial protein for HCC cell survival. Taken together, our study describes a new, non-coding RNA regulator of HCC. |
| first_indexed | 2024-09-23T08:43:56Z |
| format | Article |
| id | mit-1721.1/133329 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:43:56Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1333292021-10-28T04:54:39Z Identification of a long non-coding RNA regulator of liver carcinoma cell survival Rybakova, Yulia Gonzalez, John T Bogorad, Roman Chauhan, Vikash P Dong, Yize L Whittaker, Charles A Zatsepin, Timofei Koteliansky, Victor Anderson, Daniel G © 2021, The Author(s). Genomic studies have significantly improved our understanding of hepatocellular carcinoma (HCC) biology and have led to the discovery of multiple protein-coding genes driving hepatocarcinogenesis. In addition, these studies have identified thousands of new non-coding transcripts deregulated in HCC. We hypothesize that some of these transcripts may be involved in disease progression. Long non-coding RNAs are a large class of non-coding transcripts which participate in the regulation of virtually all cellular functions. However, a majority of lncRNAs remain dramatically understudied. Here, we applied a pooled shRNA-based screen to identify lncRNAs essential for HCC cell survival. We validated our screening results using RNAi, CRISPRi, and antisense oligonucleotides. We found a lncRNA, termed ASTILCS, that is critical for HCC cell growth and is overexpressed in tumors from HCC patients. We demonstrated that HCC cell death upon ASTILCS knockdown is associated with apoptosis induction and downregulation of a neighboring gene, protein tyrosine kinase 2 (PTK2), a crucial protein for HCC cell survival. Taken together, our study describes a new, non-coding RNA regulator of HCC. 2021-10-27T19:52:09Z 2021-10-27T19:52:09Z 2021 2021-06-04T17:47:30Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/133329 en 10.1038/s41419-021-03453-w Cell Death and Disease Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Rybakova, Yulia Gonzalez, John T Bogorad, Roman Chauhan, Vikash P Dong, Yize L Whittaker, Charles A Zatsepin, Timofei Koteliansky, Victor Anderson, Daniel G Identification of a long non-coding RNA regulator of liver carcinoma cell survival |
| title | Identification of a long non-coding RNA regulator of liver carcinoma cell survival |
| title_full | Identification of a long non-coding RNA regulator of liver carcinoma cell survival |
| title_fullStr | Identification of a long non-coding RNA regulator of liver carcinoma cell survival |
| title_full_unstemmed | Identification of a long non-coding RNA regulator of liver carcinoma cell survival |
| title_short | Identification of a long non-coding RNA regulator of liver carcinoma cell survival |
| title_sort | identification of a long non coding rna regulator of liver carcinoma cell survival |
| url | https://hdl.handle.net/1721.1/133329 |
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