Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure
Copyright: © Vasuri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. We evaluated the long...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Impact Journals, LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/133598.2 |
| _version_ | 1811079124066762752 |
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| author | Vasuri, Francesco Degiovanni, Alessio Gargiulo, Mauro Thilly, William G Gostjeva, Elena V. Pasquinelli, Gianandrea Fittipaldi, Silvia |
| author2 | Massachusetts Institute of Technology. Laboratory in Metakaryotic Biology |
| author_facet | Massachusetts Institute of Technology. Laboratory in Metakaryotic Biology Vasuri, Francesco Degiovanni, Alessio Gargiulo, Mauro Thilly, William G Gostjeva, Elena V. Pasquinelli, Gianandrea Fittipaldi, Silvia |
| author_sort | Vasuri, Francesco |
| collection | MIT |
| description | Copyright: © Vasuri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. We evaluated the long-term effects of sirolimus on three different cell in vitro models, cultured in physiological conditions mimicking sirolimus-eluted stent, in order to clarify the effectiveness of sirolimus in blocking cell proliferation and survival. Three cells lines (WPMY-1 myofibroblasts, HT-29 colorectal adenocarcinoma, and U2OS osteosarcoma) were selected and growth in 10 ml of Minimum Essential Medium for 5 weeks with serial dilutions of sirolimus. The number of colonies and the number of cells per colony were counted. As main result, the number of WPMY-1 surviving colonies increased in a dose-dependent manner when treated with sirolimus (p = 0.0011), while the number of U2OS colonies progressively decreased (p = 0.0011). The clonal capacity of HT-29 was not modified by the exposure to sirolimus (p = 0.6679). In conclusion sirolimus showed the well-known cytostatic effect, but with an effect on clonogenic potential different among the different cell types. In the practice, the plaque typology and composition may influence the response to sirolimus and thus the effectiveness of eluted stent. |
| first_indexed | 2024-09-23T11:10:25Z |
| format | Article |
| id | mit-1721.1/133598.2 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:10:25Z |
| publishDate | 2021 |
| publisher | Impact Journals, LLC |
| record_format | dspace |
| spelling | mit-1721.1/133598.22021-12-06T14:11:09Z Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure Vasuri, Francesco Degiovanni, Alessio Gargiulo, Mauro Thilly, William G Gostjeva, Elena V. Pasquinelli, Gianandrea Fittipaldi, Silvia Massachusetts Institute of Technology. Laboratory in Metakaryotic Biology Massachusetts Institute of Technology. Department of Biological Engineering Copyright: © Vasuri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. We evaluated the long-term effects of sirolimus on three different cell in vitro models, cultured in physiological conditions mimicking sirolimus-eluted stent, in order to clarify the effectiveness of sirolimus in blocking cell proliferation and survival. Three cells lines (WPMY-1 myofibroblasts, HT-29 colorectal adenocarcinoma, and U2OS osteosarcoma) were selected and growth in 10 ml of Minimum Essential Medium for 5 weeks with serial dilutions of sirolimus. The number of colonies and the number of cells per colony were counted. As main result, the number of WPMY-1 surviving colonies increased in a dose-dependent manner when treated with sirolimus (p = 0.0011), while the number of U2OS colonies progressively decreased (p = 0.0011). The clonal capacity of HT-29 was not modified by the exposure to sirolimus (p = 0.6679). In conclusion sirolimus showed the well-known cytostatic effect, but with an effect on clonogenic potential different among the different cell types. In the practice, the plaque typology and composition may influence the response to sirolimus and thus the effectiveness of eluted stent. 2021-12-06T14:11:08Z 2021-10-27T19:53:44Z 2021-12-06T14:11:08Z 2020 2021-09-10T15:04:10Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/133598.2 en 10.18632/ONCOTARGET.27554 Oncotarget Creative Commons Attribution 3.0 unported license https://creativecommons.org/licenses/by/3.0/ application/octet-stream Impact Journals, LLC Oncotarget |
| spellingShingle | Vasuri, Francesco Degiovanni, Alessio Gargiulo, Mauro Thilly, William G Gostjeva, Elena V. Pasquinelli, Gianandrea Fittipaldi, Silvia Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure |
| title | Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure |
| title_full | Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure |
| title_fullStr | Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure |
| title_full_unstemmed | Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure |
| title_short | Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure |
| title_sort | sirolimus eluting stents opposite in vitro effects on the clonogenic cell potential on a long term exposure |
| url | https://hdl.handle.net/1721.1/133598.2 |
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