Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics
© 2020 The Author(s) In order to analyze how a signal transduction network converts cellular inputs into cellular outputs, ideally one would measure the dynamics of many signals within the network simultaneously. We found that, by fusing a fluorescent reporter to a pair of self-assembling peptides,...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| 其他作者: | |
| 格式: | 文件 |
| 语言: | English |
| 出版: |
Elsevier BV
2021
|
| 在线阅读: | https://hdl.handle.net/1721.1/133667 |
| _version_ | 1826217664784105472 |
|---|---|
| author | Linghu, Changyang Johnson, Shannon L Valdes, Pablo A Shemesh, Or A Park, Won Min Park, Demian Piatkevich, Kiryl D Wassie, Asmamaw T Liu, Yixi An, Bobae Barnes, Stephanie A Celiker, Orhan T Yao, Chun-Chen Yu, Chih-Chieh Jay Wang, Ru Adamala, Katarzyna P Bear, Mark F Keating, Amy E Boyden, Edward S |
| author2 | Program in Media Arts and Sciences (Massachusetts Institute of Technology) |
| author_facet | Program in Media Arts and Sciences (Massachusetts Institute of Technology) Linghu, Changyang Johnson, Shannon L Valdes, Pablo A Shemesh, Or A Park, Won Min Park, Demian Piatkevich, Kiryl D Wassie, Asmamaw T Liu, Yixi An, Bobae Barnes, Stephanie A Celiker, Orhan T Yao, Chun-Chen Yu, Chih-Chieh Jay Wang, Ru Adamala, Katarzyna P Bear, Mark F Keating, Amy E Boyden, Edward S |
| author_sort | Linghu, Changyang |
| collection | MIT |
| description | © 2020 The Author(s) In order to analyze how a signal transduction network converts cellular inputs into cellular outputs, ideally one would measure the dynamics of many signals within the network simultaneously. We found that, by fusing a fluorescent reporter to a pair of self-assembling peptides, it could be stably clustered within cells at random points, distant enough to be resolved by a microscope but close enough to spatially sample the relevant biology. Because such clusters, which we call signaling reporter islands (SiRIs), can be modularly designed, they permit a set of fluorescent reporters to be efficiently adapted for simultaneous measurement of multiple nodes of a signal transduction network within single cells. We created SiRIs for indicators of second messengers and kinases and used them, in hippocampal neurons in culture and intact brain slices, to discover relationships between the speed of calcium signaling, and the amplitude of PKA signaling, upon receiving a cAMP-driving stimulus. |
| first_indexed | 2024-09-23T17:07:18Z |
| format | Article |
| id | mit-1721.1/133667 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T17:07:18Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1336672024-01-02T20:09:23Z Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics Linghu, Changyang Johnson, Shannon L Valdes, Pablo A Shemesh, Or A Park, Won Min Park, Demian Piatkevich, Kiryl D Wassie, Asmamaw T Liu, Yixi An, Bobae Barnes, Stephanie A Celiker, Orhan T Yao, Chun-Chen Yu, Chih-Chieh Jay Wang, Ru Adamala, Katarzyna P Bear, Mark F Keating, Amy E Boyden, Edward S Program in Media Arts and Sciences (Massachusetts Institute of Technology) McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Center for Neurobiological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Howard Hughes Medical Institute © 2020 The Author(s) In order to analyze how a signal transduction network converts cellular inputs into cellular outputs, ideally one would measure the dynamics of many signals within the network simultaneously. We found that, by fusing a fluorescent reporter to a pair of self-assembling peptides, it could be stably clustered within cells at random points, distant enough to be resolved by a microscope but close enough to spatially sample the relevant biology. Because such clusters, which we call signaling reporter islands (SiRIs), can be modularly designed, they permit a set of fluorescent reporters to be efficiently adapted for simultaneous measurement of multiple nodes of a signal transduction network within single cells. We created SiRIs for indicators of second messengers and kinases and used them, in hippocampal neurons in culture and intact brain slices, to discover relationships between the speed of calcium signaling, and the amplitude of PKA signaling, upon receiving a cAMP-driving stimulus. 2021-10-27T19:54:04Z 2021-10-27T19:54:04Z 2020 2021-03-11T18:25:52Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/133667 en 10.1016/j.cell.2020.10.035 Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV Elsevier |
| spellingShingle | Linghu, Changyang Johnson, Shannon L Valdes, Pablo A Shemesh, Or A Park, Won Min Park, Demian Piatkevich, Kiryl D Wassie, Asmamaw T Liu, Yixi An, Bobae Barnes, Stephanie A Celiker, Orhan T Yao, Chun-Chen Yu, Chih-Chieh Jay Wang, Ru Adamala, Katarzyna P Bear, Mark F Keating, Amy E Boyden, Edward S Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics |
| title | Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics |
| title_full | Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics |
| title_fullStr | Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics |
| title_full_unstemmed | Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics |
| title_short | Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics |
| title_sort | spatial multiplexing of fluorescent reporters for imaging signaling network dynamics |
| url | https://hdl.handle.net/1721.1/133667 |
| work_keys_str_mv | AT linghuchangyang spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT johnsonshannonl spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT valdespabloa spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT shemeshora spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT parkwonmin spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT parkdemian spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT piatkevichkiryld spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT wassieasmamawt spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT liuyixi spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT anbobae spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT barnesstephaniea spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT celikerorhant spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT yaochunchen spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT yuchihchiehjay spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT wangru spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT adamalakatarzynap spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT bearmarkf spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT keatingamye spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics AT boydenedwards spatialmultiplexingoffluorescentreportersforimagingsignalingnetworkdynamics |