Toward engineered processes for sequencing-based analysis of single circulating tumor cells
Sequencing-based analysis of single circulating tumor cells (CTCs) has the potential to revolutionize our understanding of metastatic cancer and improve clinical care. Technologies exist to enrich, identify, recover, and sequence single cells, but to enable systematic routine analysis of single CTCs...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2021
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| Online Access: | https://hdl.handle.net/1721.1/133927 |
| _version_ | 1826194551015997440 |
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| author | Adalsteinsson, Viktor A Love, J Christopher |
| author2 | Koch Institute for Integrative Cancer Research at MIT |
| author_facet | Koch Institute for Integrative Cancer Research at MIT Adalsteinsson, Viktor A Love, J Christopher |
| author_sort | Adalsteinsson, Viktor A |
| collection | MIT |
| description | Sequencing-based analysis of single circulating tumor cells (CTCs) has the potential to revolutionize our understanding of metastatic cancer and improve clinical care. Technologies exist to enrich, identify, recover, and sequence single cells, but to enable systematic routine analysis of single CTCs from a range of cancer patients, there is a need to establish processes that efficiently integrate these specific operations. Such engineered processes should address challenges associated with the yield and viability of enriched CTCs, the robust identification of candidate single CTCs with minimal degradation of DNA, the bias in whole-genome amplification, and the efficient handling of candidate single CTCs or their amplified DNA products. Advances in methods for single-cell analysis and nanoscale technologies suggest opportunities to overcome these challenges, and could create integrated platforms that perform several of the unit operations together. Ultimately, technologies should be selected or adapted for optimal performance and compatibility in an integrated process. © 2014 Elsevier Ltd. |
| first_indexed | 2024-09-23T09:57:59Z |
| format | Article |
| id | mit-1721.1/133927 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:57:59Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1339272023-02-17T15:27:55Z Toward engineered processes for sequencing-based analysis of single circulating tumor cells Adalsteinsson, Viktor A Love, J Christopher Koch Institute for Integrative Cancer Research at MIT Massachusetts Institute of Technology. Department of Chemical Engineering Sequencing-based analysis of single circulating tumor cells (CTCs) has the potential to revolutionize our understanding of metastatic cancer and improve clinical care. Technologies exist to enrich, identify, recover, and sequence single cells, but to enable systematic routine analysis of single CTCs from a range of cancer patients, there is a need to establish processes that efficiently integrate these specific operations. Such engineered processes should address challenges associated with the yield and viability of enriched CTCs, the robust identification of candidate single CTCs with minimal degradation of DNA, the bias in whole-genome amplification, and the efficient handling of candidate single CTCs or their amplified DNA products. Advances in methods for single-cell analysis and nanoscale technologies suggest opportunities to overcome these challenges, and could create integrated platforms that perform several of the unit operations together. Ultimately, technologies should be selected or adapted for optimal performance and compatibility in an integrated process. © 2014 Elsevier Ltd. 2021-10-27T19:57:15Z 2021-10-27T19:57:15Z 2014 2019-09-09T17:43:27Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/133927 Adalsteinsson, Viktor A., and J. Christopher Love. "Toward Engineered Processes for Sequencing-Based Analysis of Single Circulating Tumor Cells." Current Opinion in Chemical Engineering 4 (2014): 97-104. en 10.1016/J.COCHE.2014.01.011 Current Opinion in Chemical Engineering Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Adalsteinsson, Viktor A Love, J Christopher Toward engineered processes for sequencing-based analysis of single circulating tumor cells |
| title | Toward engineered processes for sequencing-based analysis of single circulating tumor cells |
| title_full | Toward engineered processes for sequencing-based analysis of single circulating tumor cells |
| title_fullStr | Toward engineered processes for sequencing-based analysis of single circulating tumor cells |
| title_full_unstemmed | Toward engineered processes for sequencing-based analysis of single circulating tumor cells |
| title_short | Toward engineered processes for sequencing-based analysis of single circulating tumor cells |
| title_sort | toward engineered processes for sequencing based analysis of single circulating tumor cells |
| url | https://hdl.handle.net/1721.1/133927 |
| work_keys_str_mv | AT adalsteinssonviktora towardengineeredprocessesforsequencingbasedanalysisofsinglecirculatingtumorcells AT lovejchristopher towardengineeredprocessesforsequencingbasedanalysisofsinglecirculatingtumorcells |