Differential translation of mRNA isoforms transcribed with distinct sigma factors
Sigma factors are an important class of bacterial transcription factors that lend specificity to RNA polymerases by binding to distinct promoter elements for genes in their regulons. Here we show that activation of the general stress sigma factor, σB, in Bacillus subtilis paradoxically leads to dram...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Cold Spring Harbor Laboratory
2021
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| Online Access: | https://hdl.handle.net/1721.1/133973 |
| _version_ | 1811091877640798208 |
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| author | McCormick, Dylan M Lalanne, Jean-Benoît Lan, Tammy CT Rouskin, Silvi Li, Gene-Wei |
| author_facet | McCormick, Dylan M Lalanne, Jean-Benoît Lan, Tammy CT Rouskin, Silvi Li, Gene-Wei |
| author_sort | McCormick, Dylan M |
| collection | MIT |
| description | Sigma factors are an important class of bacterial transcription factors that lend specificity to RNA polymerases by binding to distinct promoter elements for genes in their regulons. Here we show that activation of the general stress sigma factor, σB, in Bacillus subtilis paradoxically leads to dramatic induction of translation for a subset of its regulon genes. These genes are translationally repressed when transcribed by the housekeeping sigma factor, σA, owing to extended RNA secondary structures as determined in vivo using DMS-MaPseq. Transcription from σB-dependent promoters ablates the secondary structures and activates translation, leading to dual induction. Translation efficiencies between σB- and σA-dependent RNA isoforms can vary by up to 100-fold, which in multiple cases exceeds the magnitude of transcriptional induction. These results highlight the role of long-range RNA folding in modulating translation and demonstrate that a transcription factor can regulate protein synthesis beyond its effects on transcript levels. |
| first_indexed | 2024-09-23T15:09:26Z |
| format | Article |
| id | mit-1721.1/133973 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:09:26Z |
| publishDate | 2021 |
| publisher | Cold Spring Harbor Laboratory |
| record_format | dspace |
| spelling | mit-1721.1/1339732022-05-11T03:52:23Z Differential translation of mRNA isoforms transcribed with distinct sigma factors McCormick, Dylan M Lalanne, Jean-Benoît Lan, Tammy CT Rouskin, Silvi Li, Gene-Wei Sigma factors are an important class of bacterial transcription factors that lend specificity to RNA polymerases by binding to distinct promoter elements for genes in their regulons. Here we show that activation of the general stress sigma factor, σB, in Bacillus subtilis paradoxically leads to dramatic induction of translation for a subset of its regulon genes. These genes are translationally repressed when transcribed by the housekeeping sigma factor, σA, owing to extended RNA secondary structures as determined in vivo using DMS-MaPseq. Transcription from σB-dependent promoters ablates the secondary structures and activates translation, leading to dual induction. Translation efficiencies between σB- and σA-dependent RNA isoforms can vary by up to 100-fold, which in multiple cases exceeds the magnitude of transcriptional induction. These results highlight the role of long-range RNA folding in modulating translation and demonstrate that a transcription factor can regulate protein synthesis beyond its effects on transcript levels. 2021-10-27T19:57:27Z 2021-10-27T19:57:27Z 2021 2021-07-21T15:06:47Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/133973 en 10.1261/rna.078747.121 RNA Creative Commons Attribution NonCommercial License 4.0 https://creativecommons.org/licenses/by-nc/4.0/ application/pdf Cold Spring Harbor Laboratory Cold Spring Harbor Laboratory Press |
| spellingShingle | McCormick, Dylan M Lalanne, Jean-Benoît Lan, Tammy CT Rouskin, Silvi Li, Gene-Wei Differential translation of mRNA isoforms transcribed with distinct sigma factors |
| title | Differential translation of mRNA isoforms transcribed with distinct sigma factors |
| title_full | Differential translation of mRNA isoforms transcribed with distinct sigma factors |
| title_fullStr | Differential translation of mRNA isoforms transcribed with distinct sigma factors |
| title_full_unstemmed | Differential translation of mRNA isoforms transcribed with distinct sigma factors |
| title_short | Differential translation of mRNA isoforms transcribed with distinct sigma factors |
| title_sort | differential translation of mrna isoforms transcribed with distinct sigma factors |
| url | https://hdl.handle.net/1721.1/133973 |
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