Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase
© 2019 Wittenborn et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. The nickel-dependent carbon monoxide dehydrogenase (CODH) employs a unique heterometallic nickel-iron-sulfur cluster, termed the C-cluster, to catalyze the interconversion...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2021
|
| Online Access: | https://hdl.handle.net/1721.1/134370 |
| _version_ | 1811075578717011968 |
|---|---|
| author | Wittenborn, Elizabeth C Cohen, Steven E Merrouch, Mériem Léger, Christophe Fourmond, Vincent Dementin, Sébastien Drennan, Catherine L |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Wittenborn, Elizabeth C Cohen, Steven E Merrouch, Mériem Léger, Christophe Fourmond, Vincent Dementin, Sébastien Drennan, Catherine L |
| author_sort | Wittenborn, Elizabeth C |
| collection | MIT |
| description | © 2019 Wittenborn et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. The nickel-dependent carbon monoxide dehydrogenase (CODH) employs a unique heterometallic nickel-iron-sulfur cluster, termed the C-cluster, to catalyze the interconversion of CO and CO2. Like other complex metalloenzymes, CODH requires dedicated assembly machinery to form the fully intact and functional C-cluster. In particular, nickel incorporation into the C-cluster depends on the maturation factor CooC; however, the mechanism of nickel insertion remains poorly understood. Here, we compare X-ray structures (1.50 -2.48 Å resolution) of CODH from Desulfovibrio vulgaris (DvCODH) heterologously expressed in either the absence (DvCODH-CooC) or presence (DvCODH-CooC) of co-expressed CooC. We find that the C-cluster of DvCODH-CooC is fully loaded with iron but does not contain any nickel. Interestingly, the so-called unique iron ion (Feu) occupies both its canonical site (80% occupancy) and the nickel site (20% occupancy), with addition of reductant causing further mismetallation of the nickel site (60% iron occupancy). We also demonstrate that a DvCODH variant that lacks a surface-accessible iron- sulfur cluster (the D-cluster) has a C-cluster that is also replete in iron but lacks nickel, despite co-expression with CooC. In this variant, all Feu is in its canonical location, and the nickel site is empty. This D-cluster- deficient CODH is inactive despite attempts to reconstitute it with nickel. Taken together, these results suggest that an empty nickel site is not sufficient for nickel incorporation. Based on our findings, we propose a model for C-cluster assembly that requires both CooC and a functioning D-cluster, involves precise redoxstate control, and includes a two-step nickel-binding process. |
| first_indexed | 2024-09-23T10:08:40Z |
| format | Article |
| id | mit-1721.1/134370 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:08:40Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1343702023-09-06T20:21:46Z Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase Wittenborn, Elizabeth C Cohen, Steven E Merrouch, Mériem Léger, Christophe Fourmond, Vincent Dementin, Sébastien Drennan, Catherine L Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Biology Howard Hughes Medical Institute © 2019 Wittenborn et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. The nickel-dependent carbon monoxide dehydrogenase (CODH) employs a unique heterometallic nickel-iron-sulfur cluster, termed the C-cluster, to catalyze the interconversion of CO and CO2. Like other complex metalloenzymes, CODH requires dedicated assembly machinery to form the fully intact and functional C-cluster. In particular, nickel incorporation into the C-cluster depends on the maturation factor CooC; however, the mechanism of nickel insertion remains poorly understood. Here, we compare X-ray structures (1.50 -2.48 Å resolution) of CODH from Desulfovibrio vulgaris (DvCODH) heterologously expressed in either the absence (DvCODH-CooC) or presence (DvCODH-CooC) of co-expressed CooC. We find that the C-cluster of DvCODH-CooC is fully loaded with iron but does not contain any nickel. Interestingly, the so-called unique iron ion (Feu) occupies both its canonical site (80% occupancy) and the nickel site (20% occupancy), with addition of reductant causing further mismetallation of the nickel site (60% iron occupancy). We also demonstrate that a DvCODH variant that lacks a surface-accessible iron- sulfur cluster (the D-cluster) has a C-cluster that is also replete in iron but lacks nickel, despite co-expression with CooC. In this variant, all Feu is in its canonical location, and the nickel site is empty. This D-cluster- deficient CODH is inactive despite attempts to reconstitute it with nickel. Taken together, these results suggest that an empty nickel site is not sufficient for nickel incorporation. Based on our findings, we propose a model for C-cluster assembly that requires both CooC and a functioning D-cluster, involves precise redoxstate control, and includes a two-step nickel-binding process. 2021-10-27T20:04:40Z 2021-10-27T20:04:40Z 2019 2021-07-16T12:03:03Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/134370 en 10.1074/JBC.RA119.009610 Journal of Biological Chemistry Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Elsevier BV Elsevier |
| spellingShingle | Wittenborn, Elizabeth C Cohen, Steven E Merrouch, Mériem Léger, Christophe Fourmond, Vincent Dementin, Sébastien Drennan, Catherine L Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase |
| title | Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase |
| title_full | Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase |
| title_fullStr | Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase |
| title_full_unstemmed | Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase |
| title_short | Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase |
| title_sort | structural insight into metallocofactor maturation in carbon monoxide dehydrogenase |
| url | https://hdl.handle.net/1721.1/134370 |
| work_keys_str_mv | AT wittenbornelizabethc structuralinsightintometallocofactormaturationincarbonmonoxidedehydrogenase AT cohenstevene structuralinsightintometallocofactormaturationincarbonmonoxidedehydrogenase AT merrouchmeriem structuralinsightintometallocofactormaturationincarbonmonoxidedehydrogenase AT legerchristophe structuralinsightintometallocofactormaturationincarbonmonoxidedehydrogenase AT fourmondvincent structuralinsightintometallocofactormaturationincarbonmonoxidedehydrogenase AT dementinsebastien structuralinsightintometallocofactormaturationincarbonmonoxidedehydrogenase AT drennancatherinel structuralinsightintometallocofactormaturationincarbonmonoxidedehydrogenase |