Exploring cellular biochemistry with nanobodies
© 2020 Cheloha et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. Reagents that bind tightly and specifically to biomolecules of interest remain essential in the exploration of biology and in their ultimate application to medicine. Besides l...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2021
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| Online Access: | https://hdl.handle.net/1721.1/134413 |
| _version_ | 1826195817266937856 |
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| author | Cheloha, Ross W Harmand, Thibault J Wijne, Charlotte Schwartz, Thomas U Ploegh, Hidde L |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Cheloha, Ross W Harmand, Thibault J Wijne, Charlotte Schwartz, Thomas U Ploegh, Hidde L |
| author_sort | Cheloha, Ross W |
| collection | MIT |
| description | © 2020 Cheloha et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. Reagents that bind tightly and specifically to biomolecules of interest remain essential in the exploration of biology and in their ultimate application to medicine. Besides ligands for receptors of known specificity, agents commonly used for this purpose are monoclonal antibodies derived from mice, rabbits, and other animals. However, such antibodies can be expensive to produce, challenging to engineer, and are not necessarily stable in the context of the cellular cytoplasm, a reducing environment. Heavy chain-only antibodies, discovered in camelids, have been truncated to yield single-domain antibody fragments (VHHs or nanobodies) that overcome many of these shortcomings. Whereas they are known as crystallization chaperones for membrane proteins or as simple alternatives to conventional antibodies, nanobodies have been applied in settings where the use of standard antibodies or their derivatives would be impractical or impossible. We review recent examples in which the unique properties of nanobodies have been combined with complementary methods, such as chemical functionalization, to provide tools with unique and useful properties. |
| first_indexed | 2024-09-23T10:15:57Z |
| format | Article |
| id | mit-1721.1/134413 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:15:57Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1344132023-12-13T15:52:31Z Exploring cellular biochemistry with nanobodies Cheloha, Ross W Harmand, Thibault J Wijne, Charlotte Schwartz, Thomas U Ploegh, Hidde L Massachusetts Institute of Technology. Department of Biology © 2020 Cheloha et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. Reagents that bind tightly and specifically to biomolecules of interest remain essential in the exploration of biology and in their ultimate application to medicine. Besides ligands for receptors of known specificity, agents commonly used for this purpose are monoclonal antibodies derived from mice, rabbits, and other animals. However, such antibodies can be expensive to produce, challenging to engineer, and are not necessarily stable in the context of the cellular cytoplasm, a reducing environment. Heavy chain-only antibodies, discovered in camelids, have been truncated to yield single-domain antibody fragments (VHHs or nanobodies) that overcome many of these shortcomings. Whereas they are known as crystallization chaperones for membrane proteins or as simple alternatives to conventional antibodies, nanobodies have been applied in settings where the use of standard antibodies or their derivatives would be impractical or impossible. We review recent examples in which the unique properties of nanobodies have been combined with complementary methods, such as chemical functionalization, to provide tools with unique and useful properties. 2021-10-27T20:04:54Z 2021-10-27T20:04:54Z 2020 2021-08-02T17:27:07Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/134413 en 10.1074/JBC.REV120.012960 Journal of Biological Chemistry Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Elsevier BV American Society for Biochemistry & Molecular Biology (ASBMB) |
| spellingShingle | Cheloha, Ross W Harmand, Thibault J Wijne, Charlotte Schwartz, Thomas U Ploegh, Hidde L Exploring cellular biochemistry with nanobodies |
| title | Exploring cellular biochemistry with nanobodies |
| title_full | Exploring cellular biochemistry with nanobodies |
| title_fullStr | Exploring cellular biochemistry with nanobodies |
| title_full_unstemmed | Exploring cellular biochemistry with nanobodies |
| title_short | Exploring cellular biochemistry with nanobodies |
| title_sort | exploring cellular biochemistry with nanobodies |
| url | https://hdl.handle.net/1721.1/134413 |
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