Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions
Background: Inside the body, cells lie in direct contact or in close proximity to other cell types in a tightly controlled architecture that often regulates the resulting tissue function. Therefore, tissue engineering constructs that aim to reproduce the architecture and the geometry of tissues will...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2021
|
| Online Access: | https://hdl.handle.net/1721.1/134466 |
| _version_ | 1826204277581807616 |
|---|---|
| author | Kaji, Hirokazu Camci-Unal, Gulden Langer, Robert Khademhosseini, Ali |
| author_facet | Kaji, Hirokazu Camci-Unal, Gulden Langer, Robert Khademhosseini, Ali |
| author_sort | Kaji, Hirokazu |
| collection | MIT |
| description | Background: Inside the body, cells lie in direct contact or in close proximity to other cell types in a tightly controlled architecture that often regulates the resulting tissue function. Therefore, tissue engineering constructs that aim to reproduce the architecture and the geometry of tissues will benefit from methods of controlling cell-cell interactions with microscale resolution. Scope of the review: We discuss the use of microfabrication technologies for generating patterned co-cultures. In addition, we categorize patterned co-culture systems by cell type and discuss the implications of regulating cell-cell interactions in the resulting biological function of the tissues. Major conclusions: Patterned co-cultures are a useful tool for fabricating tissue engineered constructs and for studying cell-cell interactions in vitro, because they can be used to control the degree of homotypic and heterotypic cell-cell contact. In addition, this approach can be manipulated to elucidate important factors involved in cell-matrix interactions. General significance: Patterned co-culture strategies hold significant potential to develop biomimetic structures for tissue engineering. It is expected that they would create opportunities to develop artificial tissues in the future. This article is part of a Special Issue entitled Nanotechnologies - Emerging Applications in Biomedicine. © 2010 Elsevier B.V. |
| first_indexed | 2024-09-23T12:51:45Z |
| format | Article |
| id | mit-1721.1/134466 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:51:45Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1344662022-03-31T14:23:08Z Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions Kaji, Hirokazu Camci-Unal, Gulden Langer, Robert Khademhosseini, Ali Background: Inside the body, cells lie in direct contact or in close proximity to other cell types in a tightly controlled architecture that often regulates the resulting tissue function. Therefore, tissue engineering constructs that aim to reproduce the architecture and the geometry of tissues will benefit from methods of controlling cell-cell interactions with microscale resolution. Scope of the review: We discuss the use of microfabrication technologies for generating patterned co-cultures. In addition, we categorize patterned co-culture systems by cell type and discuss the implications of regulating cell-cell interactions in the resulting biological function of the tissues. Major conclusions: Patterned co-cultures are a useful tool for fabricating tissue engineered constructs and for studying cell-cell interactions in vitro, because they can be used to control the degree of homotypic and heterotypic cell-cell contact. In addition, this approach can be manipulated to elucidate important factors involved in cell-matrix interactions. General significance: Patterned co-culture strategies hold significant potential to develop biomimetic structures for tissue engineering. It is expected that they would create opportunities to develop artificial tissues in the future. This article is part of a Special Issue entitled Nanotechnologies - Emerging Applications in Biomedicine. © 2010 Elsevier B.V. 2021-10-27T20:05:08Z 2021-10-27T20:05:08Z 2011 2019-09-04T17:02:36Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/134466 Kaji, H., et al. "Engineering Systems for the Generation of Patterned Co-Cultures for Controlling Cell-Cell Interactions." Biochimica Et Biophysica Acta-General Subjects 1810 3 (2011): 239-50. en 10.1016/j.bbagen.2010.07.002 Biochimica et Biophysica Acta - General Subjects Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Kaji, Hirokazu Camci-Unal, Gulden Langer, Robert Khademhosseini, Ali Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions |
| title | Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions |
| title_full | Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions |
| title_fullStr | Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions |
| title_full_unstemmed | Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions |
| title_short | Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions |
| title_sort | engineering systems for the generation of patterned co cultures for controlling cell cell interactions |
| url | https://hdl.handle.net/1721.1/134466 |
| work_keys_str_mv | AT kajihirokazu engineeringsystemsforthegenerationofpatternedcoculturesforcontrollingcellcellinteractions AT camciunalgulden engineeringsystemsforthegenerationofpatternedcoculturesforcontrollingcellcellinteractions AT langerrobert engineeringsystemsforthegenerationofpatternedcoculturesforcontrollingcellcellinteractions AT khademhosseiniali engineeringsystemsforthegenerationofpatternedcoculturesforcontrollingcellcellinteractions |