Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy
For advanced treatment of diseases such as cancer, multicomponent, multifunctional nanoparticles hold great promise. In the current study we report the synthesis of a complex nanoparticle (NP) system with dual drug loading as well as diagnostic properties. To that aim we present a methodology where...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Chemical Society (ACS)
2021
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| Online Access: | https://hdl.handle.net/1721.1/134494 |
| _version_ | 1826201638734397440 |
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| author | Mieszawska, Aneta J Kim, YongTae Gianella, Anita van Rooy, Inge Priem, Bram Labarre, Matthew P Ozcan, Canturk Cormode, David P Petrov, Artiom Langer, Robert Farokhzad, Omid C Fayad, Zahi A Mulder, Willem JM |
| author2 | Koch Institute for Integrative Cancer Research at MIT |
| author_facet | Koch Institute for Integrative Cancer Research at MIT Mieszawska, Aneta J Kim, YongTae Gianella, Anita van Rooy, Inge Priem, Bram Labarre, Matthew P Ozcan, Canturk Cormode, David P Petrov, Artiom Langer, Robert Farokhzad, Omid C Fayad, Zahi A Mulder, Willem JM |
| author_sort | Mieszawska, Aneta J |
| collection | MIT |
| description | For advanced treatment of diseases such as cancer, multicomponent, multifunctional nanoparticles hold great promise. In the current study we report the synthesis of a complex nanoparticle (NP) system with dual drug loading as well as diagnostic properties. To that aim we present a methodology where chemically modified poly(lactic-co-glycolic) acid (PLGA) polymer is formulated into a polymer-lipid NP that contains a cytotoxic drug doxorubicin (DOX) in the polymeric core and an anti-angiogenic drug sorafenib (SRF) in the lipidic corona. The NP core also contains gold nanocrystals (AuNCs) for imaging purposes and cyclodextrin molecules to maximize the DOX encapsulation in the NP core. In addition, a near-infrared (NIR) Cy7 dye was incorporated in the coating. To fabricate the NP we used a microfluidics-based technique that offers unique NP synthesis conditions, which allowed for encapsulation and fine-tuning of optimal ratios of all the NP components. NP phantoms could be visualized with computed tomography (CT) and near-infrared (NIR) fluorescence imaging. We observed timed release of the encapsulated drugs, with fast release of the corona drug SRF and delayed release of a core drug DOX. In tumor bearing mice intravenously administered NPs were found to accumulate at the tumor site by fluorescence imaging. © 2013 American Chemical Society. |
| first_indexed | 2024-09-23T11:54:29Z |
| format | Article |
| id | mit-1721.1/134494 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:54:29Z |
| publishDate | 2021 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1344942023-03-01T15:28:59Z Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy Mieszawska, Aneta J Kim, YongTae Gianella, Anita van Rooy, Inge Priem, Bram Labarre, Matthew P Ozcan, Canturk Cormode, David P Petrov, Artiom Langer, Robert Farokhzad, Omid C Fayad, Zahi A Mulder, Willem JM Koch Institute for Integrative Cancer Research at MIT Massachusetts Institute of Technology. Department of Chemical Engineering Harvard University--MIT Division of Health Sciences and Technology For advanced treatment of diseases such as cancer, multicomponent, multifunctional nanoparticles hold great promise. In the current study we report the synthesis of a complex nanoparticle (NP) system with dual drug loading as well as diagnostic properties. To that aim we present a methodology where chemically modified poly(lactic-co-glycolic) acid (PLGA) polymer is formulated into a polymer-lipid NP that contains a cytotoxic drug doxorubicin (DOX) in the polymeric core and an anti-angiogenic drug sorafenib (SRF) in the lipidic corona. The NP core also contains gold nanocrystals (AuNCs) for imaging purposes and cyclodextrin molecules to maximize the DOX encapsulation in the NP core. In addition, a near-infrared (NIR) Cy7 dye was incorporated in the coating. To fabricate the NP we used a microfluidics-based technique that offers unique NP synthesis conditions, which allowed for encapsulation and fine-tuning of optimal ratios of all the NP components. NP phantoms could be visualized with computed tomography (CT) and near-infrared (NIR) fluorescence imaging. We observed timed release of the encapsulated drugs, with fast release of the corona drug SRF and delayed release of a core drug DOX. In tumor bearing mice intravenously administered NPs were found to accumulate at the tumor site by fluorescence imaging. © 2013 American Chemical Society. 2021-10-27T20:05:16Z 2021-10-27T20:05:16Z 2013 2019-09-05T15:59:53Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/134494 Mieszawska, A. J., et al. "Synthesis of Polymer-Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy." Bioconjugate Chemistry 24 9 (2013): 1429-34. en 10.1021/BC400166J Bioconjugate Chemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Mieszawska, Aneta J Kim, YongTae Gianella, Anita van Rooy, Inge Priem, Bram Labarre, Matthew P Ozcan, Canturk Cormode, David P Petrov, Artiom Langer, Robert Farokhzad, Omid C Fayad, Zahi A Mulder, Willem JM Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy |
| title | Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy |
| title_full | Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy |
| title_fullStr | Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy |
| title_full_unstemmed | Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy |
| title_short | Synthesis of Polymer–Lipid Nanoparticles for Image-Guided Delivery of Dual Modality Therapy |
| title_sort | synthesis of polymer lipid nanoparticles for image guided delivery of dual modality therapy |
| url | https://hdl.handle.net/1721.1/134494 |
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