Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics
High-density lipoprotein (HDL) is a natural nanoparticle that transports peripheral cholesterol to the liver. Reconstituted high-density lipoprotein (rHDL) exhibits antiatherothrombotic properties and is being considered as a natural treatment for cardiovascular diseases. Furthermore, HDL nanopartic...
| Main Authors: | , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society (ACS)
2021
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| Online Access: | https://hdl.handle.net/1721.1/134496 |
| _version_ | 1826207315901022208 |
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| author | Kim, YongTae Fay, Francois Cormode, David P Sanchez-Gaytan, Brenda L Tang, Jun Hennessy, Elizabeth J Ma, Mingming Moore, Kathryn Farokhzad, Omid C Fisher, Edward Allen Mulder, Willem JM Langer, Robert Fayad, Zahi A |
| author2 | Koch Institute for Integrative Cancer Research at MIT |
| author_facet | Koch Institute for Integrative Cancer Research at MIT Kim, YongTae Fay, Francois Cormode, David P Sanchez-Gaytan, Brenda L Tang, Jun Hennessy, Elizabeth J Ma, Mingming Moore, Kathryn Farokhzad, Omid C Fisher, Edward Allen Mulder, Willem JM Langer, Robert Fayad, Zahi A |
| author_sort | Kim, YongTae |
| collection | MIT |
| description | High-density lipoprotein (HDL) is a natural nanoparticle that transports peripheral cholesterol to the liver. Reconstituted high-density lipoprotein (rHDL) exhibits antiatherothrombotic properties and is being considered as a natural treatment for cardiovascular diseases. Furthermore, HDL nanoparticle platforms have been created for targeted delivery of therapeutic and diagnostic agents. The current methods for HDL reconstitution involve lengthy procedures that are challenging to scale up. A central need in the synthesis of rHDL, and multifunctional nanomaterials in general, is to establish large-scale production of reproducible and homogeneous batches in a simple and efficient fashion. Here, we present a large-scale microfluidics-based manufacturing method for single-step synthesis of HDL-mimicking nanomaterials (μHDL). μHDL is shown to have the same properties (e.g., size, morphology, bioactivity) as conventionally reconstituted HDL and native HDL. In addition, we were able to incorporate simvastatin (a hydrophobic drug) into μHDL, as well as gold, iron oxide, quantum dot nanocrystals or fluorophores to enable its detection by computed tomography (CT), magnetic resonance imaging (MRI), or fluorescence microscopy, respectively. Our approach may contribute to effective development and optimization of lipoprotein-based nanomaterials for medical imaging and drug delivery. © 2013 American Chemical Society. |
| first_indexed | 2024-09-23T13:47:26Z |
| format | Article |
| id | mit-1721.1/134496 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:47:26Z |
| publishDate | 2021 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1344962023-09-13T17:37:44Z Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics Kim, YongTae Fay, Francois Cormode, David P Sanchez-Gaytan, Brenda L Tang, Jun Hennessy, Elizabeth J Ma, Mingming Moore, Kathryn Farokhzad, Omid C Fisher, Edward Allen Mulder, Willem JM Langer, Robert Fayad, Zahi A Koch Institute for Integrative Cancer Research at MIT High-density lipoprotein (HDL) is a natural nanoparticle that transports peripheral cholesterol to the liver. Reconstituted high-density lipoprotein (rHDL) exhibits antiatherothrombotic properties and is being considered as a natural treatment for cardiovascular diseases. Furthermore, HDL nanoparticle platforms have been created for targeted delivery of therapeutic and diagnostic agents. The current methods for HDL reconstitution involve lengthy procedures that are challenging to scale up. A central need in the synthesis of rHDL, and multifunctional nanomaterials in general, is to establish large-scale production of reproducible and homogeneous batches in a simple and efficient fashion. Here, we present a large-scale microfluidics-based manufacturing method for single-step synthesis of HDL-mimicking nanomaterials (μHDL). μHDL is shown to have the same properties (e.g., size, morphology, bioactivity) as conventionally reconstituted HDL and native HDL. In addition, we were able to incorporate simvastatin (a hydrophobic drug) into μHDL, as well as gold, iron oxide, quantum dot nanocrystals or fluorophores to enable its detection by computed tomography (CT), magnetic resonance imaging (MRI), or fluorescence microscopy, respectively. Our approach may contribute to effective development and optimization of lipoprotein-based nanomaterials for medical imaging and drug delivery. © 2013 American Chemical Society. 2021-10-27T20:05:16Z 2021-10-27T20:05:16Z 2013 2019-09-05T15:56:46Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/134496 Kim, Y., et al. "Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics." ACS Nano 7 11 (2013): 9975-83. en 10.1021/NN4039063 ACS Nano Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Kim, YongTae Fay, Francois Cormode, David P Sanchez-Gaytan, Brenda L Tang, Jun Hennessy, Elizabeth J Ma, Mingming Moore, Kathryn Farokhzad, Omid C Fisher, Edward Allen Mulder, Willem JM Langer, Robert Fayad, Zahi A Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics |
| title | Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics |
| title_full | Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics |
| title_fullStr | Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics |
| title_full_unstemmed | Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics |
| title_short | Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics |
| title_sort | single step reconstitution of multifunctional high density lipoprotein derived nanomaterials using microfluidics |
| url | https://hdl.handle.net/1721.1/134496 |
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