Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting
© 2016 IOP Publishing Ltd. Vascular endothelial cells are known to respond to a range of biochemical and time-varying mechanical cues that can promote blood vessel sprouting termed angiogenesis. It is less understood how these cells respond to sustained (i.e., static) mechanical cues such as the def...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2021
|
| Online Access: | https://hdl.handle.net/1721.1/134533 |
| _version_ | 1811091152169861120 |
|---|---|
| author | Zeiger, AS Liu, FD Durham, JT Jagielska, A Mahmoodian, R Van Vliet, KJ Herman, IM |
| author2 | Massachusetts Institute of Technology. Department of Materials Science and Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Materials Science and Engineering Zeiger, AS Liu, FD Durham, JT Jagielska, A Mahmoodian, R Van Vliet, KJ Herman, IM |
| author_sort | Zeiger, AS |
| collection | MIT |
| description | © 2016 IOP Publishing Ltd. Vascular endothelial cells are known to respond to a range of biochemical and time-varying mechanical cues that can promote blood vessel sprouting termed angiogenesis. It is less understood how these cells respond to sustained (i.e., static) mechanical cues such as the deformation generated by other contractile vascular cells, cues which can change with age and disease state. Here we demonstrate that static tensile strain of 10%, consistent with that exerted by contractile microvascular pericytes, can directly and rapidly induce cell cycle re-entry in growth-arrested microvascular endothelial cell monolayers. S-phase entry in response to this strain correlates with absence of nuclear p27, a cyclin-dependent kinase inhibitor. Furthermore, this modest strain promotes sprouting of endothelial cells, suggesting a novel mechanical 'angiogenic switch'. These findings suggest that static tensile strain can directly stimulate pathological angiogenesis, implying that pericyte absence or death is not necessarily required of endothelial cell re-activation. |
| first_indexed | 2024-09-23T14:57:49Z |
| format | Article |
| id | mit-1721.1/134533 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:57:49Z |
| publishDate | 2021 |
| publisher | IOP Publishing |
| record_format | dspace |
| spelling | mit-1721.1/1345332023-03-15T19:16:15Z Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting Zeiger, AS Liu, FD Durham, JT Jagielska, A Mahmoodian, R Van Vliet, KJ Herman, IM Massachusetts Institute of Technology. Department of Materials Science and Engineering Massachusetts Institute of Technology. Department of Biological Engineering © 2016 IOP Publishing Ltd. Vascular endothelial cells are known to respond to a range of biochemical and time-varying mechanical cues that can promote blood vessel sprouting termed angiogenesis. It is less understood how these cells respond to sustained (i.e., static) mechanical cues such as the deformation generated by other contractile vascular cells, cues which can change with age and disease state. Here we demonstrate that static tensile strain of 10%, consistent with that exerted by contractile microvascular pericytes, can directly and rapidly induce cell cycle re-entry in growth-arrested microvascular endothelial cell monolayers. S-phase entry in response to this strain correlates with absence of nuclear p27, a cyclin-dependent kinase inhibitor. Furthermore, this modest strain promotes sprouting of endothelial cells, suggesting a novel mechanical 'angiogenic switch'. These findings suggest that static tensile strain can directly stimulate pathological angiogenesis, implying that pericyte absence or death is not necessarily required of endothelial cell re-activation. 2021-10-27T20:05:26Z 2021-10-27T20:05:26Z 2016 2019-09-24T17:47:54Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/134533 Zeiger, A. S., et al. "Static Mechanical Strain Induces Capillary Endothelial Cell Cycle Re-Entry and Sprouting." Phys Biol 13 4 (2016): 046006. en 10.1088/1478-3975/13/4/046006 Physical Biology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf IOP Publishing PMC |
| spellingShingle | Zeiger, AS Liu, FD Durham, JT Jagielska, A Mahmoodian, R Van Vliet, KJ Herman, IM Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting |
| title | Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting |
| title_full | Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting |
| title_fullStr | Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting |
| title_full_unstemmed | Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting |
| title_short | Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting |
| title_sort | static mechanical strain induces capillary endothelial cell cycle re entry and sprouting |
| url | https://hdl.handle.net/1721.1/134533 |
| work_keys_str_mv | AT zeigeras staticmechanicalstraininducescapillaryendothelialcellcyclereentryandsprouting AT liufd staticmechanicalstraininducescapillaryendothelialcellcyclereentryandsprouting AT durhamjt staticmechanicalstraininducescapillaryendothelialcellcyclereentryandsprouting AT jagielskaa staticmechanicalstraininducescapillaryendothelialcellcyclereentryandsprouting AT mahmoodianr staticmechanicalstraininducescapillaryendothelialcellcyclereentryandsprouting AT vanvlietkj staticmechanicalstraininducescapillaryendothelialcellcyclereentryandsprouting AT hermanim staticmechanicalstraininducescapillaryendothelialcellcyclereentryandsprouting |