Application of a dense gas technique for sterilizing soft biomaterials
Sterilization of soft biomaterials such as hydrogels is challenging because existing methods such as gamma irradiation, steam sterilization, or ethylene oxide sterilization, while effective at achieving high sterility assurance levels (SAL), may compromise their physicochemical properties and biocom...
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| 格式: | 文件 |
| 语言: | English |
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Wiley
2021
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| 在线阅读: | https://hdl.handle.net/1721.1/134535 |
| _version_ | 1826208560080486400 |
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| author | Karajanagi, Sandeep S Yoganathan, Roshan Mammucari, Raffaella Park, Hyoungshin Cox, Julian Zeitels, Steven M Langer, Robert Foster, Neil R |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Karajanagi, Sandeep S Yoganathan, Roshan Mammucari, Raffaella Park, Hyoungshin Cox, Julian Zeitels, Steven M Langer, Robert Foster, Neil R |
| author_sort | Karajanagi, Sandeep S |
| collection | MIT |
| description | Sterilization of soft biomaterials such as hydrogels is challenging because existing methods such as gamma irradiation, steam sterilization, or ethylene oxide sterilization, while effective at achieving high sterility assurance levels (SAL), may compromise their physicochemical properties and biocompatibility. New methods that effectively sterilize soft biomaterials without compromising their properties are therefore required. In this report, a dense-carbon dioxide (CO2)-based technique was used to sterilize soft polyethylene glycol (PEG)-based hydrogels while retaining their structure and physicochemical properties. Conventional sterilization methods such as gamma irradiation and steam sterilization severely compromised the structure of the hydrogels. PEG hydrogels with high water content and low elastic shear modulus (a measure of stiffness) were deliberately inoculated with bacteria and spores and then subjected to dense CO2. The dense CO2-based methods effectively sterilized the hydrogels achieving a SAL of 10-7 without compromising the viscoelastic properties, pH, water-content, and structure of the gels. Furthermore, dense CO2-treated gels were biocompatible and non-toxic when implanted subcutaneously in ferrets. The application of novel dense CO2-based methods to sterilize soft biomaterials has implications in developing safe sterilization methods for soft biomedical implants such as dermal fillers and viscosupplements. © 2011 Wiley Periodicals, Inc. |
| first_indexed | 2024-09-23T14:07:33Z |
| format | Article |
| id | mit-1721.1/134535 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:07:33Z |
| publishDate | 2021 |
| publisher | Wiley |
| record_format | dspace |
| spelling | mit-1721.1/1345352023-02-23T20:38:29Z Application of a dense gas technique for sterilizing soft biomaterials Karajanagi, Sandeep S Yoganathan, Roshan Mammucari, Raffaella Park, Hyoungshin Cox, Julian Zeitels, Steven M Langer, Robert Foster, Neil R Massachusetts Institute of Technology. Department of Chemical Engineering Harvard University--MIT Division of Health Sciences and Technology Sterilization of soft biomaterials such as hydrogels is challenging because existing methods such as gamma irradiation, steam sterilization, or ethylene oxide sterilization, while effective at achieving high sterility assurance levels (SAL), may compromise their physicochemical properties and biocompatibility. New methods that effectively sterilize soft biomaterials without compromising their properties are therefore required. In this report, a dense-carbon dioxide (CO2)-based technique was used to sterilize soft polyethylene glycol (PEG)-based hydrogels while retaining their structure and physicochemical properties. Conventional sterilization methods such as gamma irradiation and steam sterilization severely compromised the structure of the hydrogels. PEG hydrogels with high water content and low elastic shear modulus (a measure of stiffness) were deliberately inoculated with bacteria and spores and then subjected to dense CO2. The dense CO2-based methods effectively sterilized the hydrogels achieving a SAL of 10-7 without compromising the viscoelastic properties, pH, water-content, and structure of the gels. Furthermore, dense CO2-treated gels were biocompatible and non-toxic when implanted subcutaneously in ferrets. The application of novel dense CO2-based methods to sterilize soft biomaterials has implications in developing safe sterilization methods for soft biomedical implants such as dermal fillers and viscosupplements. © 2011 Wiley Periodicals, Inc. 2021-10-27T20:05:27Z 2021-10-27T20:05:27Z 2011 2019-09-04T16:48:44Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/134535 Karajanagi, S. S., et al. "Application of a Dense Gas Technique for Sterilizing Soft Biomaterials." Biotechnol Bioeng (2011). en 10.1002/bit.23105 Biotechnology and Bioengineering Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley PMC |
| spellingShingle | Karajanagi, Sandeep S Yoganathan, Roshan Mammucari, Raffaella Park, Hyoungshin Cox, Julian Zeitels, Steven M Langer, Robert Foster, Neil R Application of a dense gas technique for sterilizing soft biomaterials |
| title | Application of a dense gas technique for sterilizing soft biomaterials |
| title_full | Application of a dense gas technique for sterilizing soft biomaterials |
| title_fullStr | Application of a dense gas technique for sterilizing soft biomaterials |
| title_full_unstemmed | Application of a dense gas technique for sterilizing soft biomaterials |
| title_short | Application of a dense gas technique for sterilizing soft biomaterials |
| title_sort | application of a dense gas technique for sterilizing soft biomaterials |
| url | https://hdl.handle.net/1721.1/134535 |
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