Infection of laboratory colonies of Anopheles mosquitoes with Plasmodium vivax from cryopreserved clinical isolates

© 2016 Australian Society for Parasitology Plasmodium vivax is the most geographically widespread malaria parasite. Unique features of transmission biology complicate P. vivax control. Interventions targeting transmission are required for malaria eradication. In the absence of an in vitro culture, t...

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Bibliographic Details
Main Authors: Shaw-Saliba, Kathryn, Clarke, David, Santos, Jorge M, Menezes, Maria José, Lim, Caeul, Mascarenhas, Anjali, Chery, Laura, Gomes, Edwin, March, Sandra, Bhatia, Sangeeta N, Rathod, Pradipsinh K, Ferreira, Marcelo U, Catteruccia, Flaminia, Duraisingh, Manoj T
Other Authors: Harvard University--MIT Division of Health Sciences and Technology
Format: Article
Language:English
Published: Elsevier BV 2021
Online Access:https://hdl.handle.net/1721.1/134665
Description
Summary:© 2016 Australian Society for Parasitology Plasmodium vivax is the most geographically widespread malaria parasite. Unique features of transmission biology complicate P. vivax control. Interventions targeting transmission are required for malaria eradication. In the absence of an in vitro culture, transmission studies rely on live isolates from non-human primates or endemic regions. Here, we demonstrate P. vivax gametocytes from both India and Brazil are stable during cryopreservation. Importantly, cryopreserved gametocytes from Brazil were capable of infecting three anopheline mosquito species in feedings done in the United States. These findings create new opportunities for transmission studies in diverse locales.