In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species
© 2018, The Author(s). Helicobacter (H.) pylori is an important risk factor for gastric malignancies worldwide. Its outer membrane proteome takes an important role in colonization of the human gastric mucosa. However, in zoonotic non-H. pylori helicobacters (NHPHs) also associated with human gastric...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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Springer Nature
2021
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| Online Access: | https://hdl.handle.net/1721.1/134700 |
| _version_ | 1811085876558561280 |
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| author | Bauwens, Eva Joosten, Myrthe Taganna, Joemar Rossi, Mirko Debraekeleer, Ayla Tay, Alfred Peters, Fanny Backert, Steffen Fox, James Ducatelle, Richard Remaut, Han Haesebrouck, Freddy Smet, Annemieke |
| author2 | Massachusetts Institute of Technology. Division of Comparative Medicine |
| author_facet | Massachusetts Institute of Technology. Division of Comparative Medicine Bauwens, Eva Joosten, Myrthe Taganna, Joemar Rossi, Mirko Debraekeleer, Ayla Tay, Alfred Peters, Fanny Backert, Steffen Fox, James Ducatelle, Richard Remaut, Han Haesebrouck, Freddy Smet, Annemieke |
| author_sort | Bauwens, Eva |
| collection | MIT |
| description | © 2018, The Author(s). Helicobacter (H.) pylori is an important risk factor for gastric malignancies worldwide. Its outer membrane proteome takes an important role in colonization of the human gastric mucosa. However, in zoonotic non-H. pylori helicobacters (NHPHs) also associated with human gastric disease, the composition of the outer membrane (OM) proteome and its relative contribution to disease remain largely unknown. By means of a comprehensive survey of the diversity and distribution of predicted outer membrane proteins (OMPs) identified in all known gastric Helicobacter species with fully annotated genome sequences, we found genus- and species-specific families known or thought to be implicated in virulence. Hop adhesins, part of the Helicobacter-specific family 13 (Hop, Hor and Hom) were restricted to the gastric species H. pylori, H. cetorum and H. acinonychis. Hof proteins (family 33) were putative adhesins with predicted Occ- or MOMP-family like 18-stranded β-barrels. They were found to be widespread amongst all gastric Helicobacter species only sporadically detected in enterohepatic Helicobacter species. These latter are other members within the genus Helicobacter, although ecologically and genetically distinct. LpxR, a lipopolysaccharide remodeling factor, was also detected in all gastric Helicobacter species but lacking as well from the enterohepatic species H. cinaedi, H. equorum and H. hepaticus. In conclusion, our systemic survey of Helicobacter OMPs points to species and infection-site specific members that are interesting candidates for future virulence and colonization studies. |
| first_indexed | 2024-09-23T13:16:57Z |
| format | Article |
| id | mit-1721.1/134700 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:16:57Z |
| publishDate | 2021 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1347002023-11-03T15:42:09Z In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species Bauwens, Eva Joosten, Myrthe Taganna, Joemar Rossi, Mirko Debraekeleer, Ayla Tay, Alfred Peters, Fanny Backert, Steffen Fox, James Ducatelle, Richard Remaut, Han Haesebrouck, Freddy Smet, Annemieke Massachusetts Institute of Technology. Division of Comparative Medicine © 2018, The Author(s). Helicobacter (H.) pylori is an important risk factor for gastric malignancies worldwide. Its outer membrane proteome takes an important role in colonization of the human gastric mucosa. However, in zoonotic non-H. pylori helicobacters (NHPHs) also associated with human gastric disease, the composition of the outer membrane (OM) proteome and its relative contribution to disease remain largely unknown. By means of a comprehensive survey of the diversity and distribution of predicted outer membrane proteins (OMPs) identified in all known gastric Helicobacter species with fully annotated genome sequences, we found genus- and species-specific families known or thought to be implicated in virulence. Hop adhesins, part of the Helicobacter-specific family 13 (Hop, Hor and Hom) were restricted to the gastric species H. pylori, H. cetorum and H. acinonychis. Hof proteins (family 33) were putative adhesins with predicted Occ- or MOMP-family like 18-stranded β-barrels. They were found to be widespread amongst all gastric Helicobacter species only sporadically detected in enterohepatic Helicobacter species. These latter are other members within the genus Helicobacter, although ecologically and genetically distinct. LpxR, a lipopolysaccharide remodeling factor, was also detected in all gastric Helicobacter species but lacking as well from the enterohepatic species H. cinaedi, H. equorum and H. hepaticus. In conclusion, our systemic survey of Helicobacter OMPs points to species and infection-site specific members that are interesting candidates for future virulence and colonization studies. 2021-10-27T20:08:43Z 2021-10-27T20:08:43Z 2018 2019-02-15T15:52:51Z Article http://purl.org/eprint/type/JournalArticle 2045-2322 https://hdl.handle.net/1721.1/134700 Bauwens, Eva, Myrthe Joosten, Joemar Taganna, Mirko Rossi, Ayla Debraekeleer, Alfred Tay, Fanny Peters, et al. “In Silico Proteomic and Phylogenetic Analysis of the Outer Membrane Protein Repertoire of Gastric Helicobacter Species.” Scientific Reports 8, no. 1 (October 18, 2018). doi:10.1038/s41598-018-32476-1. http://dx.doi.org/10.1038/s41598-018-32476-1 Scientific Reports Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Nature Scientific Reports |
| spellingShingle | Bauwens, Eva Joosten, Myrthe Taganna, Joemar Rossi, Mirko Debraekeleer, Ayla Tay, Alfred Peters, Fanny Backert, Steffen Fox, James Ducatelle, Richard Remaut, Han Haesebrouck, Freddy Smet, Annemieke In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species |
| title | In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species |
| title_full | In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species |
| title_fullStr | In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species |
| title_full_unstemmed | In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species |
| title_short | In silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric Helicobacter species |
| title_sort | in silico proteomic and phylogenetic analysis of the outer membrane protein repertoire of gastric helicobacter species |
| url | https://hdl.handle.net/1721.1/134700 |
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