Pressure-Modulated Selective Electrokinetic Trapping for Direct Enrichment, Purification, and Detection of Nucleic Acids in Human Serum

Copyright © 2018 American Chemical Society. Micro total-analysis systems (μTAS) have been extensively developed for the detection of nucleic acids (NAs) in resource-limited settings in recent years, yet the sample-preparation steps that interface real-world samples with on-chip analytics remain as t...

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Auteurs principaux: Ouyang, Wei, Li, Zirui, Han, Jongyoon
Autres auteurs: Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Format: Article
Langue:English
Publié: American Chemical Society (ACS) 2021
Accès en ligne:https://hdl.handle.net/1721.1/134740
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author Ouyang, Wei
Li, Zirui
Han, Jongyoon
author2 Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
author_facet Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Ouyang, Wei
Li, Zirui
Han, Jongyoon
author_sort Ouyang, Wei
collection MIT
description Copyright © 2018 American Chemical Society. Micro total-analysis systems (μTAS) have been extensively developed for the detection of nucleic acids (NAs) in resource-limited settings in recent years, yet the sample-preparation steps that interface real-world samples with on-chip analytics remain as the technical bottleneck. We report pressure-modulated selective electrokinetic trapping (PM-SET) for the direct enrichment, purification, and detection of NAs in human serum in one step without involving tedious solid-phase extraction, chemical amplification, and surface-hybridization-based assays. Under appropriately modulated hydrostatic pressures, NAs in human serum were selectively enriched in an electrokinetic concentrator with the majority of background proteins removed, achieving an enrichment factor of >4800 in 15 min. A sequence-specific NA was detected simultaneously during the enrichment process using a complementary morpholino (MO) probe, realizing a limit of detection of 3 pM in 15 min. PM-SET greatly reduces the cost, time, and complexity of sample preparation for NA detection and could be easily interfaced with existing NA-detection devices to achieve true sample-to-answer biomolecular analytics.
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spelling mit-1721.1/1347402023-01-11T18:57:03Z Pressure-Modulated Selective Electrokinetic Trapping for Direct Enrichment, Purification, and Detection of Nucleic Acids in Human Serum Ouyang, Wei Li, Zirui Han, Jongyoon Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Massachusetts Institute of Technology. Department of Biological Engineering Copyright © 2018 American Chemical Society. Micro total-analysis systems (μTAS) have been extensively developed for the detection of nucleic acids (NAs) in resource-limited settings in recent years, yet the sample-preparation steps that interface real-world samples with on-chip analytics remain as the technical bottleneck. We report pressure-modulated selective electrokinetic trapping (PM-SET) for the direct enrichment, purification, and detection of NAs in human serum in one step without involving tedious solid-phase extraction, chemical amplification, and surface-hybridization-based assays. Under appropriately modulated hydrostatic pressures, NAs in human serum were selectively enriched in an electrokinetic concentrator with the majority of background proteins removed, achieving an enrichment factor of >4800 in 15 min. A sequence-specific NA was detected simultaneously during the enrichment process using a complementary morpholino (MO) probe, realizing a limit of detection of 3 pM in 15 min. PM-SET greatly reduces the cost, time, and complexity of sample preparation for NA detection and could be easily interfaced with existing NA-detection devices to achieve true sample-to-answer biomolecular analytics. 2021-10-27T20:08:55Z 2021-10-27T20:08:55Z 2018 2019-06-05T15:56:48Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/134740 en 10.1021/ACS.ANALCHEM.8B02330 Analytical Chemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) Other repository
spellingShingle Ouyang, Wei
Li, Zirui
Han, Jongyoon
Pressure-Modulated Selective Electrokinetic Trapping for Direct Enrichment, Purification, and Detection of Nucleic Acids in Human Serum
title Pressure-Modulated Selective Electrokinetic Trapping for Direct Enrichment, Purification, and Detection of Nucleic Acids in Human Serum
title_full Pressure-Modulated Selective Electrokinetic Trapping for Direct Enrichment, Purification, and Detection of Nucleic Acids in Human Serum
title_fullStr Pressure-Modulated Selective Electrokinetic Trapping for Direct Enrichment, Purification, and Detection of Nucleic Acids in Human Serum
title_full_unstemmed Pressure-Modulated Selective Electrokinetic Trapping for Direct Enrichment, Purification, and Detection of Nucleic Acids in Human Serum
title_short Pressure-Modulated Selective Electrokinetic Trapping for Direct Enrichment, Purification, and Detection of Nucleic Acids in Human Serum
title_sort pressure modulated selective electrokinetic trapping for direct enrichment purification and detection of nucleic acids in human serum
url https://hdl.handle.net/1721.1/134740
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AT lizirui pressuremodulatedselectiveelectrokinetictrappingfordirectenrichmentpurificationanddetectionofnucleicacidsinhumanserum
AT hanjongyoon pressuremodulatedselectiveelectrokinetictrappingfordirectenrichmentpurificationanddetectionofnucleicacidsinhumanserum