Expansion microscopy: development and neuroscience applications
© 2017 Elsevier Ltd Many neuroscience questions center around understanding how the molecules and wiring in neural circuits mechanistically yield behavioral functions, or go awry in disease states. However, mapping the molecules and wiring of neurons across the large scales of neural circuits has po...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Elsevier BV
2021
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| Online Access: | https://hdl.handle.net/1721.1/134926 |
| _version_ | 1826206164662091776 |
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| author | Karagiannis, Emmanouil D Boyden, Edward S |
| author2 | Massachusetts Institute of Technology. Media Laboratory |
| author_facet | Massachusetts Institute of Technology. Media Laboratory Karagiannis, Emmanouil D Boyden, Edward S |
| author_sort | Karagiannis, Emmanouil D |
| collection | MIT |
| description | © 2017 Elsevier Ltd Many neuroscience questions center around understanding how the molecules and wiring in neural circuits mechanistically yield behavioral functions, or go awry in disease states. However, mapping the molecules and wiring of neurons across the large scales of neural circuits has posed a great challenge. We recently developed expansion microscopy (ExM), a process in which we physically magnify biological specimens such as brain circuits. We synthesize throughout preserved brain specimens a dense, even mesh of a swellable polymer such as sodium polyacrylate, anchoring key biomolecules such as proteins and nucleic acids to the polymer. After mechanical homogenization of the specimen-polymer composite, we add water, and the polymer swells, pulling biomolecules apart. Due to the larger separation between molecules, ordinary microscopes can then perform nanoscale resolution imaging. We here review the ExM technology as well as applications to the mapping of synapses, cells, and circuits, including deployment in species such as Drosophila, mouse, non-human primate, and human. |
| first_indexed | 2024-09-23T13:25:06Z |
| format | Article |
| id | mit-1721.1/134926 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:25:06Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1349262023-02-17T21:43:40Z Expansion microscopy: development and neuroscience applications Karagiannis, Emmanouil D Boyden, Edward S Massachusetts Institute of Technology. Media Laboratory Massachusetts Institute of Technology. Department of Biological Engineering McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences © 2017 Elsevier Ltd Many neuroscience questions center around understanding how the molecules and wiring in neural circuits mechanistically yield behavioral functions, or go awry in disease states. However, mapping the molecules and wiring of neurons across the large scales of neural circuits has posed a great challenge. We recently developed expansion microscopy (ExM), a process in which we physically magnify biological specimens such as brain circuits. We synthesize throughout preserved brain specimens a dense, even mesh of a swellable polymer such as sodium polyacrylate, anchoring key biomolecules such as proteins and nucleic acids to the polymer. After mechanical homogenization of the specimen-polymer composite, we add water, and the polymer swells, pulling biomolecules apart. Due to the larger separation between molecules, ordinary microscopes can then perform nanoscale resolution imaging. We here review the ExM technology as well as applications to the mapping of synapses, cells, and circuits, including deployment in species such as Drosophila, mouse, non-human primate, and human. 2021-10-27T20:09:53Z 2021-10-27T20:09:53Z 2018 2019-07-19T15:59:35Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/134926 en 10.1016/J.CONB.2017.12.012 Current Opinion in Neurobiology Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Karagiannis, Emmanouil D Boyden, Edward S Expansion microscopy: development and neuroscience applications |
| title | Expansion microscopy: development and neuroscience applications |
| title_full | Expansion microscopy: development and neuroscience applications |
| title_fullStr | Expansion microscopy: development and neuroscience applications |
| title_full_unstemmed | Expansion microscopy: development and neuroscience applications |
| title_short | Expansion microscopy: development and neuroscience applications |
| title_sort | expansion microscopy development and neuroscience applications |
| url | https://hdl.handle.net/1721.1/134926 |
| work_keys_str_mv | AT karagiannisemmanouild expansionmicroscopydevelopmentandneuroscienceapplications AT boydenedwards expansionmicroscopydevelopmentandneuroscienceapplications |