Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions
© 2018 The Royal Society of Chemistry. Neutrophils are the first white blood cells to respond to microbes and to limit their invasion of the body. However, the growth of the microbes into colonies often challenges the neutrophils ability to contain them. To study the interactions between neutrophils...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Royal Society of Chemistry (RSC)
2021
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| Online Access: | https://hdl.handle.net/1721.1/135034 |
| _version_ | 1811094405863440384 |
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| author | Kim, Jae Jung Reátegui, Eduardo Hopke, Alex Jalali, Fatemeh Roushan, Maedeh Doyle, Patrick S Irimia, Daniel |
| author_facet | Kim, Jae Jung Reátegui, Eduardo Hopke, Alex Jalali, Fatemeh Roushan, Maedeh Doyle, Patrick S Irimia, Daniel |
| author_sort | Kim, Jae Jung |
| collection | MIT |
| description | © 2018 The Royal Society of Chemistry. Neutrophils are the first white blood cells to respond to microbes and to limit their invasion of the body. However, the growth of the microbes into colonies often challenges the neutrophils ability to contain them. To study the interactions between neutrophils and microbial colonies, we designed an assay for arranging microbes in clusters of controlled size (i.e. living colloids). The patterned microbes in the living colloid are mechanically trapped inside the wells and fully accessible to neutrophils. Using the assay, we studied the interactions between human neutrophils and Candida albicans and Staphylococcus aureus, two common human pathogens. We also probed the susceptibility of C. albicans colloids to caspofungin, a common antifungal drug. |
| first_indexed | 2024-09-23T15:59:33Z |
| format | Article |
| id | mit-1721.1/135034 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:59:33Z |
| publishDate | 2021 |
| publisher | Royal Society of Chemistry (RSC) |
| record_format | dspace |
| spelling | mit-1721.1/1350342022-03-31T14:31:24Z Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions Kim, Jae Jung Reátegui, Eduardo Hopke, Alex Jalali, Fatemeh Roushan, Maedeh Doyle, Patrick S Irimia, Daniel © 2018 The Royal Society of Chemistry. Neutrophils are the first white blood cells to respond to microbes and to limit their invasion of the body. However, the growth of the microbes into colonies often challenges the neutrophils ability to contain them. To study the interactions between neutrophils and microbial colonies, we designed an assay for arranging microbes in clusters of controlled size (i.e. living colloids). The patterned microbes in the living colloid are mechanically trapped inside the wells and fully accessible to neutrophils. Using the assay, we studied the interactions between human neutrophils and Candida albicans and Staphylococcus aureus, two common human pathogens. We also probed the susceptibility of C. albicans colloids to caspofungin, a common antifungal drug. 2021-10-27T20:10:25Z 2021-10-27T20:10:25Z 2018 2019-08-16T17:52:25Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135034 en 10.1039/C8LC00228B Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry (RSC) PMC |
| spellingShingle | Kim, Jae Jung Reátegui, Eduardo Hopke, Alex Jalali, Fatemeh Roushan, Maedeh Doyle, Patrick S Irimia, Daniel Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions |
| title | Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions |
| title_full | Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions |
| title_fullStr | Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions |
| title_full_unstemmed | Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions |
| title_short | Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions |
| title_sort | large scale patterning of living colloids for dynamic studies of neutrophil microbe interactions |
| url | https://hdl.handle.net/1721.1/135034 |
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