Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA
© 2019, The Author(s). Rapid bacterial identification remains a critical challenge in infectious disease diagnostics. We developed a novel molecular approach to detect and identify a wide diversity of bacterial pathogens in a single, simple assay, exploiting the conservation, abundance, and rich phy...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/135089 |
| _version_ | 1811071216112369664 |
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| author | Bhattacharyya, Roby P Walker, Mark Boykin, Rich Son, Sophie S Liu, Jamin Hachey, Austin C Ma, Peijun Wu, Lidan Choi, Kyungyong Cummins, Kaelyn C Benson, Maura Skerry, Jennifer Ryu, Hyunryul Wong, Sharon Y Goldberg, Marcia B Han, Jongyoon Pierce, Virginia M Cosimi, Lisa A Shoresh, Noam Livny, Jonathan Beechem, Joseph Hung, Deborah T |
| author2 | Massachusetts Institute of Technology. Research Laboratory of Electronics |
| author_facet | Massachusetts Institute of Technology. Research Laboratory of Electronics Bhattacharyya, Roby P Walker, Mark Boykin, Rich Son, Sophie S Liu, Jamin Hachey, Austin C Ma, Peijun Wu, Lidan Choi, Kyungyong Cummins, Kaelyn C Benson, Maura Skerry, Jennifer Ryu, Hyunryul Wong, Sharon Y Goldberg, Marcia B Han, Jongyoon Pierce, Virginia M Cosimi, Lisa A Shoresh, Noam Livny, Jonathan Beechem, Joseph Hung, Deborah T |
| author_sort | Bhattacharyya, Roby P |
| collection | MIT |
| description | © 2019, The Author(s). Rapid bacterial identification remains a critical challenge in infectious disease diagnostics. We developed a novel molecular approach to detect and identify a wide diversity of bacterial pathogens in a single, simple assay, exploiting the conservation, abundance, and rich phylogenetic content of ribosomal RNA in a rapid fluorescent hybridization assay that requires no amplification or enzymology. Of 117 isolates from 64 species across 4 phyla, this assay identified bacteria with >89% accuracy at the species level and 100% accuracy at the family level, enabling all critical clinical distinctions. In pilot studies on primary clinical specimens, including sputum, blood cultures, and pus, bacteria from 5 different phyla were identified. |
| first_indexed | 2024-09-23T08:47:45Z |
| format | Article |
| id | mit-1721.1/135089 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:47:45Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1350892023-09-28T20:12:07Z Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA Bhattacharyya, Roby P Walker, Mark Boykin, Rich Son, Sophie S Liu, Jamin Hachey, Austin C Ma, Peijun Wu, Lidan Choi, Kyungyong Cummins, Kaelyn C Benson, Maura Skerry, Jennifer Ryu, Hyunryul Wong, Sharon Y Goldberg, Marcia B Han, Jongyoon Pierce, Virginia M Cosimi, Lisa A Shoresh, Noam Livny, Jonathan Beechem, Joseph Hung, Deborah T Massachusetts Institute of Technology. Research Laboratory of Electronics Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Biological Engineering © 2019, The Author(s). Rapid bacterial identification remains a critical challenge in infectious disease diagnostics. We developed a novel molecular approach to detect and identify a wide diversity of bacterial pathogens in a single, simple assay, exploiting the conservation, abundance, and rich phylogenetic content of ribosomal RNA in a rapid fluorescent hybridization assay that requires no amplification or enzymology. Of 117 isolates from 64 species across 4 phyla, this assay identified bacteria with >89% accuracy at the species level and 100% accuracy at the family level, enabling all critical clinical distinctions. In pilot studies on primary clinical specimens, including sputum, blood cultures, and pus, bacteria from 5 different phyla were identified. 2021-10-27T20:10:40Z 2021-10-27T20:10:40Z 2019 2019-06-05T16:21:18Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135089 en 10.1038/s41598-019-40792-3 Scientific Reports Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Scientific Reports |
| spellingShingle | Bhattacharyya, Roby P Walker, Mark Boykin, Rich Son, Sophie S Liu, Jamin Hachey, Austin C Ma, Peijun Wu, Lidan Choi, Kyungyong Cummins, Kaelyn C Benson, Maura Skerry, Jennifer Ryu, Hyunryul Wong, Sharon Y Goldberg, Marcia B Han, Jongyoon Pierce, Virginia M Cosimi, Lisa A Shoresh, Noam Livny, Jonathan Beechem, Joseph Hung, Deborah T Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA |
| title | Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA |
| title_full | Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA |
| title_fullStr | Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA |
| title_full_unstemmed | Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA |
| title_short | Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA |
| title_sort | rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal rna |
| url | https://hdl.handle.net/1721.1/135089 |
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