Sensing intracellular calcium ions using a manganese-based MRI contrast agent
© 2019, The Author(s). Calcium ions are essential to signal transduction in virtually all cells, where they coordinate processes ranging from embryogenesis to neural function. Although optical probes for intracellular calcium imaging have been available for decades, the development of probes for non...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2021
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| Online Access: | https://hdl.handle.net/1721.1/135156 |
| _version_ | 1826203876727980032 |
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| author | Barandov, Ali Bartelle, Benjamin B Williamson, Catherine G Loucks, Emily S Lippard, Stephen J Jasanoff, Alan |
| author_facet | Barandov, Ali Bartelle, Benjamin B Williamson, Catherine G Loucks, Emily S Lippard, Stephen J Jasanoff, Alan |
| author_sort | Barandov, Ali |
| collection | MIT |
| description | © 2019, The Author(s). Calcium ions are essential to signal transduction in virtually all cells, where they coordinate processes ranging from embryogenesis to neural function. Although optical probes for intracellular calcium imaging have been available for decades, the development of probes for noninvasive detection of intracellular calcium signaling in deep tissue and intact organisms remains a challenge. To address this problem, we synthesized a manganese-based paramagnetic contrast agent, ManICS1-AM, designed to permeate cells, undergo esterase cleavage, and allow intracellular calcium levels to be monitored by magnetic resonance imaging (MRI). Cells loaded with ManICS1-AM show changes in MRI contrast when stimulated with pharmacological agents or optogenetic tools; responses directly parallel the signals obtained using fluorescent calcium indicators. Introduction of ManICS1-AM into rodent brains furthermore permits MRI-based measurement of neural activation in optically inaccessible brain regions. These results thus validate ManICS1-AM as a calcium sensor compatible with the extensive penetration depth and field of view afforded by MRI. |
| first_indexed | 2024-09-23T12:45:04Z |
| format | Article |
| id | mit-1721.1/135156 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T12:45:04Z |
| publishDate | 2021 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1351562022-03-29T20:43:00Z Sensing intracellular calcium ions using a manganese-based MRI contrast agent Barandov, Ali Bartelle, Benjamin B Williamson, Catherine G Loucks, Emily S Lippard, Stephen J Jasanoff, Alan © 2019, The Author(s). Calcium ions are essential to signal transduction in virtually all cells, where they coordinate processes ranging from embryogenesis to neural function. Although optical probes for intracellular calcium imaging have been available for decades, the development of probes for noninvasive detection of intracellular calcium signaling in deep tissue and intact organisms remains a challenge. To address this problem, we synthesized a manganese-based paramagnetic contrast agent, ManICS1-AM, designed to permeate cells, undergo esterase cleavage, and allow intracellular calcium levels to be monitored by magnetic resonance imaging (MRI). Cells loaded with ManICS1-AM show changes in MRI contrast when stimulated with pharmacological agents or optogenetic tools; responses directly parallel the signals obtained using fluorescent calcium indicators. Introduction of ManICS1-AM into rodent brains furthermore permits MRI-based measurement of neural activation in optically inaccessible brain regions. These results thus validate ManICS1-AM as a calcium sensor compatible with the extensive penetration depth and field of view afforded by MRI. 2021-10-27T20:11:00Z 2021-10-27T20:11:00Z 2019 2019-07-18T14:53:24Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135156 en 10.1038/S41467-019-08558-7 Nature Communications Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Barandov, Ali Bartelle, Benjamin B Williamson, Catherine G Loucks, Emily S Lippard, Stephen J Jasanoff, Alan Sensing intracellular calcium ions using a manganese-based MRI contrast agent |
| title | Sensing intracellular calcium ions using a manganese-based MRI contrast agent |
| title_full | Sensing intracellular calcium ions using a manganese-based MRI contrast agent |
| title_fullStr | Sensing intracellular calcium ions using a manganese-based MRI contrast agent |
| title_full_unstemmed | Sensing intracellular calcium ions using a manganese-based MRI contrast agent |
| title_short | Sensing intracellular calcium ions using a manganese-based MRI contrast agent |
| title_sort | sensing intracellular calcium ions using a manganese based mri contrast agent |
| url | https://hdl.handle.net/1721.1/135156 |
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