Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells
<jats:p>Differentiation of endometrial fibroblasts into specialized decidual cells controls embryo implantation and transforms the cycling endometrium into a semi-permanent, immune-protective matrix that accommodates the placenta throughout pregnancy. This process starts during the midluteal p...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media SA
2021
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| Online Access: | https://hdl.handle.net/1721.1/135277 |
| _version_ | 1811094123399086080 |
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| author | Harden, Sarah L Zhou, Jieliang Gharanei, Seley Diniz-da-Costa, Maria Lucas, Emma S Cui, Liang Murakami, Keisuke Fang, Jinling Chen, Qingfeng Brosens, Jan J Lee, Yie Hou |
| author2 | Singapore-MIT Alliance in Research and Technology (SMART) |
| author_facet | Singapore-MIT Alliance in Research and Technology (SMART) Harden, Sarah L Zhou, Jieliang Gharanei, Seley Diniz-da-Costa, Maria Lucas, Emma S Cui, Liang Murakami, Keisuke Fang, Jinling Chen, Qingfeng Brosens, Jan J Lee, Yie Hou |
| author_sort | Harden, Sarah L |
| collection | MIT |
| description | <jats:p>Differentiation of endometrial fibroblasts into specialized decidual cells controls embryo implantation and transforms the cycling endometrium into a semi-permanent, immune-protective matrix that accommodates the placenta throughout pregnancy. This process starts during the midluteal phase of the menstrual cycle with decidual transformation of perivascular cells (PVC) surrounding the terminal spiral arterioles and endometrial stromal cells (EnSC) underlying the luminal epithelium. Decidualization involves extensive cellular reprogramming and acquisition of a secretory phenotype, essential for coordinated placental trophoblast invasion. Secreted metabolites are an emerging class of signaling molecules, collectively known as the exometabolome. Here, we used liquid chromatography-mass spectrometry to characterize and analyze time-resolved changes in metabolite secretion (exometabolome) of primary PVC and EnSC decidualized over 8 days. PVC were isolated using positive selection of the cell surface marker SUSD2. We identified 79 annotated metabolites differentially secreted upon decidualization, including prostaglandin, sphingolipid, and hyaluronic acid metabolites. Secreted metabolites encompassed 21 metabolic pathways, most prominently glycerolipid and pyrimidine metabolism. Although temporal exometabolome changes were comparable between decidualizing PVC and EnSC, 32 metabolites were differentially secreted across the decidualization time-course. Further, targeted metabolomics demonstrated significant differences in secretion of purine pathway metabolites between decidualized PVC and EnSC. Taken together, our findings indicate that the metabolic footprints generated by different decidual subpopulations encode spatiotemporal information that may be important for optimal embryo implantation.</jats:p> |
| first_indexed | 2024-09-23T15:55:27Z |
| format | Article |
| id | mit-1721.1/135277 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:55:27Z |
| publishDate | 2021 |
| publisher | Frontiers Media SA |
| record_format | dspace |
| spelling | mit-1721.1/1352772023-12-06T18:10:39Z Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells Harden, Sarah L Zhou, Jieliang Gharanei, Seley Diniz-da-Costa, Maria Lucas, Emma S Cui, Liang Murakami, Keisuke Fang, Jinling Chen, Qingfeng Brosens, Jan J Lee, Yie Hou Singapore-MIT Alliance in Research and Technology (SMART) <jats:p>Differentiation of endometrial fibroblasts into specialized decidual cells controls embryo implantation and transforms the cycling endometrium into a semi-permanent, immune-protective matrix that accommodates the placenta throughout pregnancy. This process starts during the midluteal phase of the menstrual cycle with decidual transformation of perivascular cells (PVC) surrounding the terminal spiral arterioles and endometrial stromal cells (EnSC) underlying the luminal epithelium. Decidualization involves extensive cellular reprogramming and acquisition of a secretory phenotype, essential for coordinated placental trophoblast invasion. Secreted metabolites are an emerging class of signaling molecules, collectively known as the exometabolome. Here, we used liquid chromatography-mass spectrometry to characterize and analyze time-resolved changes in metabolite secretion (exometabolome) of primary PVC and EnSC decidualized over 8 days. PVC were isolated using positive selection of the cell surface marker SUSD2. We identified 79 annotated metabolites differentially secreted upon decidualization, including prostaglandin, sphingolipid, and hyaluronic acid metabolites. Secreted metabolites encompassed 21 metabolic pathways, most prominently glycerolipid and pyrimidine metabolism. Although temporal exometabolome changes were comparable between decidualizing PVC and EnSC, 32 metabolites were differentially secreted across the decidualization time-course. Further, targeted metabolomics demonstrated significant differences in secretion of purine pathway metabolites between decidualized PVC and EnSC. Taken together, our findings indicate that the metabolic footprints generated by different decidual subpopulations encode spatiotemporal information that may be important for optimal embryo implantation.</jats:p> 2021-10-27T20:22:45Z 2021-10-27T20:22:45Z 2021 2021-04-28T13:46:56Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135277 en 10.3389/fcell.2021.626619 Frontiers in Cell and Developmental Biology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. https://creativecommons.org/licenses/by/4.0/ application/pdf Frontiers Media SA Frontiers |
| spellingShingle | Harden, Sarah L Zhou, Jieliang Gharanei, Seley Diniz-da-Costa, Maria Lucas, Emma S Cui, Liang Murakami, Keisuke Fang, Jinling Chen, Qingfeng Brosens, Jan J Lee, Yie Hou Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells |
| title | Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells |
| title_full | Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells |
| title_fullStr | Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells |
| title_full_unstemmed | Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells |
| title_short | Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells |
| title_sort | exometabolomic analysis of decidualizing human endometrial stromal and perivascular cells |
| url | https://hdl.handle.net/1721.1/135277 |
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