Deformation Response of Catenated DNA Networks in a Planar Elongational Field
Copyright © 2020 American Chemical Society. A kinetoplast is a complex catenated DNA network that bears resemblance to a two-dimensional polymeric system. In this work, we use single-molecule experiments to study the transient and steady-state deformation of kinetoplasts in a planar elongational fie...
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| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society (ACS)
2021
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| Online Access: | https://hdl.handle.net/1721.1/135319 |
| _version_ | 1826216572421668864 |
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| author | Soh, Beatrice W Doyle, Patrick S |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Soh, Beatrice W Doyle, Patrick S |
| author_sort | Soh, Beatrice W |
| collection | MIT |
| description | Copyright © 2020 American Chemical Society. A kinetoplast is a complex catenated DNA network that bears resemblance to a two-dimensional polymeric system. In this work, we use single-molecule experiments to study the transient and steady-state deformation of kinetoplasts in a planar elongational field. We demonstrate that kinetoplasts deform in a stagewise manner and undergo transient deformation at large strains, due to conformational rearrangements from an intermediate metastable state. Kinetoplasts in an elongational field achieve a steady-state deformation that depends on strain rate, akin to the deformation of linear polymers. We do not observe an abrupt transition between the nondeformed and deformed states of a kinetoplast, in contrast to the coil-stretch transition for a linear polymer. |
| first_indexed | 2024-09-23T16:49:42Z |
| format | Article |
| id | mit-1721.1/135319 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:49:42Z |
| publishDate | 2021 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1353192023-01-10T19:27:09Z Deformation Response of Catenated DNA Networks in a Planar Elongational Field Soh, Beatrice W Doyle, Patrick S Massachusetts Institute of Technology. Department of Chemical Engineering Copyright © 2020 American Chemical Society. A kinetoplast is a complex catenated DNA network that bears resemblance to a two-dimensional polymeric system. In this work, we use single-molecule experiments to study the transient and steady-state deformation of kinetoplasts in a planar elongational field. We demonstrate that kinetoplasts deform in a stagewise manner and undergo transient deformation at large strains, due to conformational rearrangements from an intermediate metastable state. Kinetoplasts in an elongational field achieve a steady-state deformation that depends on strain rate, akin to the deformation of linear polymers. We do not observe an abrupt transition between the nondeformed and deformed states of a kinetoplast, in contrast to the coil-stretch transition for a linear polymer. 2021-10-27T20:22:57Z 2021-10-27T20:22:57Z 2020 2021-06-09T18:05:25Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135319 en 10.1021/ACSMACROLETT.0C00360 ACS Macro Letters Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Chemical Society (ACS) MIT web domain |
| spellingShingle | Soh, Beatrice W Doyle, Patrick S Deformation Response of Catenated DNA Networks in a Planar Elongational Field |
| title | Deformation Response of Catenated DNA Networks in a Planar Elongational Field |
| title_full | Deformation Response of Catenated DNA Networks in a Planar Elongational Field |
| title_fullStr | Deformation Response of Catenated DNA Networks in a Planar Elongational Field |
| title_full_unstemmed | Deformation Response of Catenated DNA Networks in a Planar Elongational Field |
| title_short | Deformation Response of Catenated DNA Networks in a Planar Elongational Field |
| title_sort | deformation response of catenated dna networks in a planar elongational field |
| url | https://hdl.handle.net/1721.1/135319 |
| work_keys_str_mv | AT sohbeatricew deformationresponseofcatenateddnanetworksinaplanarelongationalfield AT doylepatricks deformationresponseofcatenateddnanetworksinaplanarelongationalfield |