Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing
© 2020 The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. RNA interference (RNAi) is a gene-silencing pathway that can play roles in viral defense, transposon silencing, heterochromatin formation and post-transcriptional gene silencing. Although absent from...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press (OUP)
2022
|
| Online Access: | https://hdl.handle.net/1721.1/135467.2 |
| _version_ | 1810975322042007552 |
|---|---|
| author | Getz, Matthew Aaron Weinberg, David E. Drinnenberg, Ines A. Fink, Gerald R Bartel, David |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Getz, Matthew Aaron Weinberg, David E. Drinnenberg, Ines A. Fink, Gerald R Bartel, David |
| author_sort | Getz, Matthew Aaron |
| collection | MIT |
| description | © 2020 The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. RNA interference (RNAi) is a gene-silencing pathway that can play roles in viral defense, transposon silencing, heterochromatin formation and post-transcriptional gene silencing. Although absent from Saccharomyces cerevisiae, RNAi is present in other budding-yeast species, including Naumovozyma castellii, which have an unusual Dicer and a conventional Argonaute that are both required for gene silencing. To identify other factors that act in the budding-yeast pathway, we performed an unbiased genetic selection. This selection identified Xrn1p, the cytoplasmic 5′-to-3′ exoribonuclease, as a cofactor of RNAi in budding yeast. Deletion of XRN1 impaired gene silencing in N. castellii, and this impaired silencing was attributable to multiple functions of Xrn1p, including affecting the composition of siRNA species in the cell, influencing the efficiency of siRNA loading into Argonaute, degradation of cleaved passenger strand and degradation of sliced target RNA. |
| first_indexed | 2024-09-23T08:37:32Z |
| format | Article |
| id | mit-1721.1/135467.2 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:37:32Z |
| publishDate | 2022 |
| publisher | Oxford University Press (OUP) |
| record_format | dspace |
| spelling | mit-1721.1/135467.22022-09-22T07:07:12Z Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing Getz, Matthew Aaron Weinberg, David E. Drinnenberg, Ines A. Fink, Gerald R Bartel, David Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Howard Hughes Medical Institute © 2020 The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. RNA interference (RNAi) is a gene-silencing pathway that can play roles in viral defense, transposon silencing, heterochromatin formation and post-transcriptional gene silencing. Although absent from Saccharomyces cerevisiae, RNAi is present in other budding-yeast species, including Naumovozyma castellii, which have an unusual Dicer and a conventional Argonaute that are both required for gene silencing. To identify other factors that act in the budding-yeast pathway, we performed an unbiased genetic selection. This selection identified Xrn1p, the cytoplasmic 5′-to-3′ exoribonuclease, as a cofactor of RNAi in budding yeast. Deletion of XRN1 impaired gene silencing in N. castellii, and this impaired silencing was attributable to multiple functions of Xrn1p, including affecting the composition of siRNA species in the cell, influencing the efficiency of siRNA loading into Argonaute, degradation of cleaved passenger strand and degradation of sliced target RNA. 2022-06-30T13:51:56Z 2021-10-27T20:23:34Z 2022-06-30T13:51:56Z 2020 2021-07-14T14:24:20Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/135467.2 en 10.1093/NAR/GKAA468 Nucleic Acids Research Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/octet-stream Oxford University Press (OUP) Oxford University Press |
| spellingShingle | Getz, Matthew Aaron Weinberg, David E. Drinnenberg, Ines A. Fink, Gerald R Bartel, David Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing |
| title | Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing |
| title_full | Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing |
| title_fullStr | Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing |
| title_full_unstemmed | Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing |
| title_short | Xrn1p acts at multiple steps in the budding-yeast RNAi pathway to enhance the efficiency of silencing |
| title_sort | xrn1p acts at multiple steps in the budding yeast rnai pathway to enhance the efficiency of silencing |
| url | https://hdl.handle.net/1721.1/135467.2 |
| work_keys_str_mv | AT getzmatthewaaron xrn1pactsatmultiplestepsinthebuddingyeastrnaipathwaytoenhancetheefficiencyofsilencing AT weinbergdavide xrn1pactsatmultiplestepsinthebuddingyeastrnaipathwaytoenhancetheefficiencyofsilencing AT drinnenberginesa xrn1pactsatmultiplestepsinthebuddingyeastrnaipathwaytoenhancetheefficiencyofsilencing AT finkgeraldr xrn1pactsatmultiplestepsinthebuddingyeastrnaipathwaytoenhancetheefficiencyofsilencing AT barteldavid xrn1pactsatmultiplestepsinthebuddingyeastrnaipathwaytoenhancetheefficiencyofsilencing |